SRF - Journal of Cell Science
... Site-directed mutagenesis was carried out on double-stranded DNA according to Deng and Nickoloff (1992) with the Pharmacia USE kit. Briefly, two oligonucleotides were used: one introduces the desired mutation(s) and the second mutates a unique non-essential restriction site (ScaI) into another one ( ...
... Site-directed mutagenesis was carried out on double-stranded DNA according to Deng and Nickoloff (1992) with the Pharmacia USE kit. Briefly, two oligonucleotides were used: one introduces the desired mutation(s) and the second mutates a unique non-essential restriction site (ScaI) into another one ( ...
Recognition of Metal Ion Binding Proteins
... the neighboring non-metalloproteins, it is obvious that both the training set and the test set has to contain a set of metalloproteins and its nearest non-metal-binding neighbors in it. Furthermore since the part of the feature vector which describes the amino acid composition pays resemblance to km ...
... the neighboring non-metalloproteins, it is obvious that both the training set and the test set has to contain a set of metalloproteins and its nearest non-metal-binding neighbors in it. Furthermore since the part of the feature vector which describes the amino acid composition pays resemblance to km ...
RNA EXTRACTION
... • Homogenate (from homogenization step) must be store for 5 min at RT to - permit the complete dissociation of nucleoprotein complexes. • Chloroform used to: - Separate solution in aqueous phase, interphase and organic phase - RNA in aqueous phase, DNA (interphase) and protein (organic phase) • RNA, ...
... • Homogenate (from homogenization step) must be store for 5 min at RT to - permit the complete dissociation of nucleoprotein complexes. • Chloroform used to: - Separate solution in aqueous phase, interphase and organic phase - RNA in aqueous phase, DNA (interphase) and protein (organic phase) • RNA, ...
Caldicellulosiruptor tāpirins bind to crystalline cellulose! ! 1 Discrete
... (CBM37) to facilitate anchoring of enzymes to the cell surface (13,14). Other glycosylated proteins from R. albus also play a role in cellulose binding, including pilA1 homologs (15) from strains 8 (16) and 20 (17). Perhaps not surprisingly, type IV pili have also been demonstrated to be involved in ...
... (CBM37) to facilitate anchoring of enzymes to the cell surface (13,14). Other glycosylated proteins from R. albus also play a role in cellulose binding, including pilA1 homologs (15) from strains 8 (16) and 20 (17). Perhaps not surprisingly, type IV pili have also been demonstrated to be involved in ...
bioinorganic 1
... Hemoglobin’s active sites (all 4 of them) without the O2 is known as deoxyhemoglobin. The active site contains five-coordinate, pseudo-square planar Fe(+2). The fifth donor site is an imidazole ring from a histidine residue in the polypeptide chain. In deoxyhemoglobin the iron lies above the protopo ...
... Hemoglobin’s active sites (all 4 of them) without the O2 is known as deoxyhemoglobin. The active site contains five-coordinate, pseudo-square planar Fe(+2). The fifth donor site is an imidazole ring from a histidine residue in the polypeptide chain. In deoxyhemoglobin the iron lies above the protopo ...
(2) rRNA
... (i) Factor-dependent terminators have very little sequence in common with each other and so are not readily apparent. Fig. 2.19 illustrates a current model for factor-dependent termination. (ii) Theρfactor attaches to the mRNA at a rut (rho utilization) site if the rut region is not being translated ...
... (i) Factor-dependent terminators have very little sequence in common with each other and so are not readily apparent. Fig. 2.19 illustrates a current model for factor-dependent termination. (ii) Theρfactor attaches to the mRNA at a rut (rho utilization) site if the rut region is not being translated ...
Heat shock Proteins (HSPs)
... Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
... Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat, high pressures, and toxic compounds. It is also one of the most abundant cellular proteins found under nonstress conditions ...
Integral proteins are in
... The basis of membrane structure is a lipid bilayer To answer the question that how many lipid layers were in membrane, in 1925 Gorter and Grendel extracted the lipids from a known number of erythrocytes and spread the lipid film on a water surface. The area of lipid film on the water was about twice ...
... The basis of membrane structure is a lipid bilayer To answer the question that how many lipid layers were in membrane, in 1925 Gorter and Grendel extracted the lipids from a known number of erythrocytes and spread the lipid film on a water surface. The area of lipid film on the water was about twice ...
Lecture 1 Cell Biology
... transport proteins, called channel proteins, function by having a hydrophilic channel that certain molecules use as a tunnel through the membrane. ...
... transport proteins, called channel proteins, function by having a hydrophilic channel that certain molecules use as a tunnel through the membrane. ...
Proteins and Albumin
... respectively. Amino acid chains containing more than a dozen or so residues are often referred to as oligopeptides (from the Greek oligos, meaning “few”). Proteins are typically defined as polypeptides with a molecular weight of greater than 5000 Daltons (ie, > 5 kDa). Proteins adopt stable conforma ...
... respectively. Amino acid chains containing more than a dozen or so residues are often referred to as oligopeptides (from the Greek oligos, meaning “few”). Proteins are typically defined as polypeptides with a molecular weight of greater than 5000 Daltons (ie, > 5 kDa). Proteins adopt stable conforma ...
The Three Domains of Life:
... criteria were further analyzed. The sequences were aligned using CLUSTALW (Thompson, et.al. 1994)) and the programs NEIGHBOR JOINING DISTANCE (NJ) (which is based on the number of estimated nucleotide differences separating two proteins) (Felsenstein, 1995) and the most recently updated version of P ...
... criteria were further analyzed. The sequences were aligned using CLUSTALW (Thompson, et.al. 1994)) and the programs NEIGHBOR JOINING DISTANCE (NJ) (which is based on the number of estimated nucleotide differences separating two proteins) (Felsenstein, 1995) and the most recently updated version of P ...
The NF- B Pathway
... inducible nitric oxide synthase, which regulate the innate immune response, as well as proteins that regulate the specific immune response, such as major ...
... inducible nitric oxide synthase, which regulate the innate immune response, as well as proteins that regulate the specific immune response, such as major ...
PowerPoint Presentation - Foundations of Biology
... Rho, that binds to and slides along the RNA transcript. The terminator sequence slows down the elongation complex, Rho catches up and knocks it off the DNA Rho independent termination depends on both slowing down the elongation complex, and an AT-rich region that destabilizes the elongation complex ...
... Rho, that binds to and slides along the RNA transcript. The terminator sequence slows down the elongation complex, Rho catches up and knocks it off the DNA Rho independent termination depends on both slowing down the elongation complex, and an AT-rich region that destabilizes the elongation complex ...
An Expression and Bioinformatics Analysis of the Arabidopsis
... At3g12240 contains an aberrant carboxyterminal region. Examination of the corresponding genomic sequence revealed that the 3# end of the penultimate exon of the gene was not identified correctly during annotation, resulting in the inferred translation continuing through the final intron until a stop ...
... At3g12240 contains an aberrant carboxyterminal region. Examination of the corresponding genomic sequence revealed that the 3# end of the penultimate exon of the gene was not identified correctly during annotation, resulting in the inferred translation continuing through the final intron until a stop ...
Chapter 28 Discovery and Classification of Glycan
... A large group of GBPs that defy classification based on sequence or structure recognize sulfated GAGs (Chapter 38). The best-studied example is the interaction of heparin with antithrombin. Heparin was discovered in 1916 by Jay McLean, a medical student, but it was not until 1939 that heparin was ...
... A large group of GBPs that defy classification based on sequence or structure recognize sulfated GAGs (Chapter 38). The best-studied example is the interaction of heparin with antithrombin. Heparin was discovered in 1916 by Jay McLean, a medical student, but it was not until 1939 that heparin was ...
biography: edwin cohn
... Edwin Cohn attended the universities of Amherst and Chicago, and it was at the latter one that he decided to pursue a scientific career. After completing his undergraduate studies at the University of Chicago, Cohn received his doctorate in 1917. The scientist chose to focus his work on the study of ...
... Edwin Cohn attended the universities of Amherst and Chicago, and it was at the latter one that he decided to pursue a scientific career. After completing his undergraduate studies at the University of Chicago, Cohn received his doctorate in 1917. The scientist chose to focus his work on the study of ...
The Formation of the Central Element of the
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
Here. - Blumenstiel Lab
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
... formation. Both the N- and C-terminal deletions significantly reduce or abolish meiotic recombination similarly to c(3)G null homozygotes. To explain these data, we propose that in Drosophila the N terminus, but not the C-terminal globular domain, of C(3)G is critical for the formation of antiparall ...
sv-lncs - Department of Computer Science and Engineering
... functions could rely more on the presence of protein interactions, others on the absence of protein interactions, while others could take both into consideration, for example MSC with probability. MSC can also incorporate different methods which rely on domain interaction by using these different me ...
... functions could rely more on the presence of protein interactions, others on the absence of protein interactions, while others could take both into consideration, for example MSC with probability. MSC can also incorporate different methods which rely on domain interaction by using these different me ...
Lecture 15: Translation and Transcription
... mRNA contains a genetic message consisting of sequential codons (mRNA codon codes for one amino acid) tRNA is the interpreter between the two forms of information—base sequence in mRNA and amino acid sequence in the polypeptide ...
... mRNA contains a genetic message consisting of sequential codons (mRNA codon codes for one amino acid) tRNA is the interpreter between the two forms of information—base sequence in mRNA and amino acid sequence in the polypeptide ...
Sp3 Represses Gene Expression via the Titration of Promoter
... members and other factors account for the induced transcription of additional Sp-dependent genes remains to be determined. Several years ago we identified two novel Sp3-derived proteins, termed M1 and M2, that arise by internal translational initiation within the region of Sp3 mRNA that encodes the ...
... members and other factors account for the induced transcription of additional Sp-dependent genes remains to be determined. Several years ago we identified two novel Sp3-derived proteins, termed M1 and M2, that arise by internal translational initiation within the region of Sp3 mRNA that encodes the ...
Analysis of Fish Protein
... today. This theory is strongly supported by the discovery that a great deal of DNA sequence similarity exists among the genes of all modern-day organisms. For example, scientists were astounded to discover that the same family of genes (Hox genes) controls the embryonic development of animals as div ...
... today. This theory is strongly supported by the discovery that a great deal of DNA sequence similarity exists among the genes of all modern-day organisms. For example, scientists were astounded to discover that the same family of genes (Hox genes) controls the embryonic development of animals as div ...
Molecular Chaperones in the Cytosol: from Nascent Chain to Folded
... to the formation of structured, fibrillar aggregates, known as amyloid, that are associated with diseases such as Alzheimer’s or Huntington’s disease (6, 7) (Fig. 1). Compared to refolding in dilute solution, the tendency of nonnative states to aggregate in the cell is partially folded intermediates ...
... to the formation of structured, fibrillar aggregates, known as amyloid, that are associated with diseases such as Alzheimer’s or Huntington’s disease (6, 7) (Fig. 1). Compared to refolding in dilute solution, the tendency of nonnative states to aggregate in the cell is partially folded intermediates ...
Molecular characterization of the uncultivatable hemotropic
... which contained 550 and 523 uncharacterized hypothetical proteins (including 240 and 229 paralogs; representing 18.9% and 18.5% genome coverage) respectively. Sequence comparisons suggested that they may have arisen by gene duplication events. The predicted motifs of the majority of these putative p ...
... which contained 550 and 523 uncharacterized hypothetical proteins (including 240 and 229 paralogs; representing 18.9% and 18.5% genome coverage) respectively. Sequence comparisons suggested that they may have arisen by gene duplication events. The predicted motifs of the majority of these putative p ...
Algorithms and a Software Application for the Discovery of Heparin
... 2.1.1 Proteins and Amino Acids Proteins and amino acids are the biological compounds that comprise all living things. There are 20 amino acids found in nature, and these 20 amino acids combine uniquely to form new proteins. Thus, every protein can be unambiguously represented by a sequence of a lett ...
... 2.1.1 Proteins and Amino Acids Proteins and amino acids are the biological compounds that comprise all living things. There are 20 amino acids found in nature, and these 20 amino acids combine uniquely to form new proteins. Thus, every protein can be unambiguously represented by a sequence of a lett ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.