Membrane Proteins
... from membrane?); trans-membrane helix; antiparallel β barrel Briefly explain what “lipid rafts” are in membranes. What are primary lipid components? Explain in structural terms how an integral membrane protein can deal with its polar backbone groups in spanning the hydrophobic core of a lipid bilaye ...
... from membrane?); trans-membrane helix; antiparallel β barrel Briefly explain what “lipid rafts” are in membranes. What are primary lipid components? Explain in structural terms how an integral membrane protein can deal with its polar backbone groups in spanning the hydrophobic core of a lipid bilaye ...
Evaluation of Different Solvent Types on the Extraction of
... facilitate the release of the cell contents during the cell disruption and extraction process is undoubtedly one of the most crucial initial steps in downstream processing. It is important to enhance the assimilation and bioavailability of the intracellular compounds in the extraction solvent (Safi ...
... facilitate the release of the cell contents during the cell disruption and extraction process is undoubtedly one of the most crucial initial steps in downstream processing. It is important to enhance the assimilation and bioavailability of the intracellular compounds in the extraction solvent (Safi ...
New drugs for hepatitis C
... Lambert, S. M., Langley, D. R., Garnett, J. A., Angell, R., Hedgethorne, K., Meanwell, N. A., & Matthews, S. J. (2014). The crystal structure of NS5A domain 1 from genotype 1a reveals new clues to the mechanism of action for dimeric HCV inhibitors. Protein Science : A Publication of the Protein Soci ...
... Lambert, S. M., Langley, D. R., Garnett, J. A., Angell, R., Hedgethorne, K., Meanwell, N. A., & Matthews, S. J. (2014). The crystal structure of NS5A domain 1 from genotype 1a reveals new clues to the mechanism of action for dimeric HCV inhibitors. Protein Science : A Publication of the Protein Soci ...
BIOINFORMATICS ORIGINAL PAPER Going from where to why—interpretable prediction of protein subcellular localization
... machine learning models behind high-accuracy predictors are often very complex making it difficult to understand why a particular prediction was made. Moreover, most predictors do not provide a confidence estimate. Consequently, predictions cannot be verified with regard to their significance and re ...
... machine learning models behind high-accuracy predictors are often very complex making it difficult to understand why a particular prediction was made. Moreover, most predictors do not provide a confidence estimate. Consequently, predictions cannot be verified with regard to their significance and re ...
RNAzol RT (R4533) - Technical Bulletin - Sigma
... from DNA, protein, polysaccharides, and other molecules. RNAzol RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effecti ...
... from DNA, protein, polysaccharides, and other molecules. RNAzol RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effecti ...
Selective and specific cleavage of the D 1 and D2 proteins of
... case of photoinhibirory illumination were taken into consideration, the sizes of fragments of the DI and D2 proteins were almost the same in each of the three different treatments (Fig. 2A,B). This result suggests that the DI and D2 proteins are cleaved at identical sites in each case. This possibil ...
... case of photoinhibirory illumination were taken into consideration, the sizes of fragments of the DI and D2 proteins were almost the same in each of the three different treatments (Fig. 2A,B). This result suggests that the DI and D2 proteins are cleaved at identical sites in each case. This possibil ...
Chapter 20. Proteins
... During and after synthesis the primary sequence will associate in a fashion that leads to the most stable, "comfortable" structure for the protein. How a protein folds is largely dictated by the primary sequence of amino acids. Each amino acid in the sequence will associate with other amino acids to ...
... During and after synthesis the primary sequence will associate in a fashion that leads to the most stable, "comfortable" structure for the protein. How a protein folds is largely dictated by the primary sequence of amino acids. Each amino acid in the sequence will associate with other amino acids to ...
dependent phosphotransferase system – two highly similar glucose
... and ribosome-binding site, suggesting independent regulation. The finding of putative cis-active operator sequences, CRE (catabolite-responsive elements) suggests additional regulation by carbon catabolite repression. As described previously by the authors, both genes can be expressed in Escherichia ...
... and ribosome-binding site, suggesting independent regulation. The finding of putative cis-active operator sequences, CRE (catabolite-responsive elements) suggests additional regulation by carbon catabolite repression. As described previously by the authors, both genes can be expressed in Escherichia ...
A secondary structure motif predictive of protein localization to the
... identi®ed in the inclusion membrane (IM). The predicted IncA, IncB and IncC amino acid sequences share very limited similarity, but a common hydrophobicity motif is present within each Inc protein. In an effort to identify a relatively complete catalogue of Chlamydia trachomatis proteins present in ...
... identi®ed in the inclusion membrane (IM). The predicted IncA, IncB and IncC amino acid sequences share very limited similarity, but a common hydrophobicity motif is present within each Inc protein. In an effort to identify a relatively complete catalogue of Chlamydia trachomatis proteins present in ...
Chap. 3. "Amino Acids and the Primary Structures of Proteins
... Electrophoresis refers to the migration of charged molecules in an electric field. Molecules move toward the electrode having the opposite charge. The negatively charged electrode is called the cathode because it attracts cations. The positively charged electrode is called the anode because it attra ...
... Electrophoresis refers to the migration of charged molecules in an electric field. Molecules move toward the electrode having the opposite charge. The negatively charged electrode is called the cathode because it attracts cations. The positively charged electrode is called the anode because it attra ...
Bioinformatics analysis of experimentally determined protein
... the remaining 19 unclassified complexes could be also unambiguously classified as non-essential once a more complete list of all essential complexes becomes available. The Supplementary Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2 ...
... the remaining 19 unclassified complexes could be also unambiguously classified as non-essential once a more complete list of all essential complexes becomes available. The Supplementary Material provide detailed predictions on the characteristics of all complexes identified by Gavin et al. (Gavin 2 ...
Going from where to why—interpretable
... machine learning models behind high-accuracy predictors are often very complex making it difficult to understand why a particular prediction was made. Moreover, most predictors do not provide a confidence estimate. Consequently, predictions cannot be verified with regard to their significance and re ...
... machine learning models behind high-accuracy predictors are often very complex making it difficult to understand why a particular prediction was made. Moreover, most predictors do not provide a confidence estimate. Consequently, predictions cannot be verified with regard to their significance and re ...
Transcription and RNA processing
... Repression of gene expression Cascade regulation Dimerization ...
... Repression of gene expression Cascade regulation Dimerization ...
A metaproteomic assessment of winter and summer
... Matches to the Bacteroidetes clade ( ¼ CytophagaFlavobacterium-Bacteroides or CFB group) were mainly to Flavobacteria (54 proteins). Our data are consistent with the phylogenetic diversity obtained by previous gene-based analyses of coastal Southern Ocean waters, which identified the relative abunda ...
... Matches to the Bacteroidetes clade ( ¼ CytophagaFlavobacterium-Bacteroides or CFB group) were mainly to Flavobacteria (54 proteins). Our data are consistent with the phylogenetic diversity obtained by previous gene-based analyses of coastal Southern Ocean waters, which identified the relative abunda ...
Over Expression of IPTG inducible GST protein in E.coli BL21
... by glutathione on an electrophilic substrate [2]. The resulting glutathione S conjugate are more soluble than the original substrate and thus more easily transported from the cell, mediated by ATP dependent MAPEG family membrane glycoprotein belonging to multiple drug resistant protein family [3]. E ...
... by glutathione on an electrophilic substrate [2]. The resulting glutathione S conjugate are more soluble than the original substrate and thus more easily transported from the cell, mediated by ATP dependent MAPEG family membrane glycoprotein belonging to multiple drug resistant protein family [3]. E ...
Identification of Target Messenger RNA Substrates for the Murine
... identify mRNAs from mouse testis extract that were specifically bound by an mDAZL protein fused to the GST domain (GST-mDAZL). The immobilized GST-mDAZL fusion protein was incubated with testis total extract, and mRNP complexes bound by GST-mDAZL were extracted. Testis total extract, which contains ...
... identify mRNAs from mouse testis extract that were specifically bound by an mDAZL protein fused to the GST domain (GST-mDAZL). The immobilized GST-mDAZL fusion protein was incubated with testis total extract, and mRNP complexes bound by GST-mDAZL were extracted. Testis total extract, which contains ...
Origin of Life on Earth - Department of Molecular and Cellular Biology
... a serious paradox: it seems that it takes proteins— as well as the information now stored in DNA— to make proteins. On the other hand, the paradox would disappear if the first organisms did not require proteins at all. Recent experiments suggest it would have been possible for genetic molecules simi ...
... a serious paradox: it seems that it takes proteins— as well as the information now stored in DNA— to make proteins. On the other hand, the paradox would disappear if the first organisms did not require proteins at all. Recent experiments suggest it would have been possible for genetic molecules simi ...
BIOL562_Lecture_13
... End result of genome expression is proteome (a collection of proteins in a cell); tRNA 3’ end is attached to amino acid by aminoacylation; 5’ end is attached to mRNA by condon-anticodon interactions; wobble effect allows single tRNA read more than 1 codons. Bacterial ribosome has internal binding si ...
... End result of genome expression is proteome (a collection of proteins in a cell); tRNA 3’ end is attached to amino acid by aminoacylation; 5’ end is attached to mRNA by condon-anticodon interactions; wobble effect allows single tRNA read more than 1 codons. Bacterial ribosome has internal binding si ...
fibulins: a versatile family of extracellular matrix proteins
... are somewhat larger than their mammalian counterparts and have a lower sequence similarity. This indicates that fibulin-1 is ancient in origin and that there has been a distinct modulation of its function during evolution. Modular protein structure. Fibulin-1 was the first family member to be identi ...
... are somewhat larger than their mammalian counterparts and have a lower sequence similarity. This indicates that fibulin-1 is ancient in origin and that there has been a distinct modulation of its function during evolution. Modular protein structure. Fibulin-1 was the first family member to be identi ...
Detection of Cellular Response to an in vitro Challenge with
... processed by Proteome Discoverer software using The Mascot® search engine. Two different peptide identification strategies were used. The simple search method (Figure 3) only searches for high-confidence, tryptic peptides and phosphopeptides. The more complex search strategy (Figure 4), breaks the P ...
... processed by Proteome Discoverer software using The Mascot® search engine. Two different peptide identification strategies were used. The simple search method (Figure 3) only searches for high-confidence, tryptic peptides and phosphopeptides. The more complex search strategy (Figure 4), breaks the P ...
P1 and P2 protein heterodimer binding to the P0 protein of
... heterodimers, P1a/P2b and P1b/P2a, that preferentially bind to sites A and B of the P0 protein, respectively. Using mutant strains carrying only one of the four possible P1/P2 combinations, we found a specific phenotype associated to each P1/P2 pair, indicating that not all acidic P proteins play th ...
... heterodimers, P1a/P2b and P1b/P2a, that preferentially bind to sites A and B of the P0 protein, respectively. Using mutant strains carrying only one of the four possible P1/P2 combinations, we found a specific phenotype associated to each P1/P2 pair, indicating that not all acidic P proteins play th ...
LIFE ON EARTH
... a serious paradox: it seems that it takes proteins— as well as the information now stored in DNA— to make proteins. On the other hand, the paradox would disappear if the first organisms did not require proteins at all. Recent experiments suggest it would have been possible for genetic molecules simi ...
... a serious paradox: it seems that it takes proteins— as well as the information now stored in DNA— to make proteins. On the other hand, the paradox would disappear if the first organisms did not require proteins at all. Recent experiments suggest it would have been possible for genetic molecules simi ...
Basic Principles of Transcription and Translation
... The spliceosome cuts the pre Spliceosome mRNA releasing the intron and at components the same time splices the exons Cut-out together. The spliceosome then intron mRNA comes apart releasing mRNA ...
... The spliceosome cuts the pre Spliceosome mRNA releasing the intron and at components the same time splices the exons Cut-out together. The spliceosome then intron mRNA comes apart releasing mRNA ...
A Proteome Reference Map and Proteomic Analysis
... ppm (300 ppm in some cases) and one partial cleavage. Oxidation of methionine and carbamidomethyl modification of cysteine were considered. For unambiguous identification of proteins, more than five peptides must be matched, and the sequence coverage must be greater than 15%. Fourteen spots represen ...
... ppm (300 ppm in some cases) and one partial cleavage. Oxidation of methionine and carbamidomethyl modification of cysteine were considered. For unambiguous identification of proteins, more than five peptides must be matched, and the sequence coverage must be greater than 15%. Fourteen spots represen ...
Chapter 5, Membranes
... • Treatment of right side out vesicles with protease released carbohydrates into medium • Similar treatment of inside out vesicles did not release carbohydrate (what does this mean?) • Carbohydrates could be enzymatically labeled in right side out but not inside out vesicles • Carbohydrates on prote ...
... • Treatment of right side out vesicles with protease released carbohydrates into medium • Similar treatment of inside out vesicles did not release carbohydrate (what does this mean?) • Carbohydrates could be enzymatically labeled in right side out but not inside out vesicles • Carbohydrates on prote ...
SR protein
SR proteins are a conserved family of proteins involved in RNA splicing. SR proteins are named because they contain a protein domain with long repeats of serine and arginine amino acid residues, whose standard abbreviations are ""S"" and ""R"" respectively. SR proteins are 50-300 amino acids in length and composed of two domains, the RNA recognition motif (RRM) region and the RS binding domain. SR proteins are more commonly found in the nucleus than the cytoplasm, but several SR proteins are known to shuttle between the nucleus and the cytoplasm.SR proteins were discovered in the 1990s in Drosophila and in amphibian oocytes, and later in humans. In general, metazoans appear to have SR proteins and unicellular organisms lack SR proteins.SR proteins are important in constitutive and alternative pre-mRNA splicing, mRNA export, genome stabilization, nonsense-mediated decay, and translation. SR proteins alternatively splice pre-mRNA by preferentially selecting different splice sites on the pre-mRNA strands to create multiple mRNA transcripts from one pre-mRNA transcript. Once splicing is complete the SR protein may or may not remain attached to help shuttle the mRNA strand out of the nucleus. As RNA Polymerase II is transcribing DNA into RNA, SR proteins attach to newly made pre-mRNA to prevent the pre-mRNA from binding to the coding DNA strand to increase genome stabilization. Topoisomerase I and SR proteins also interact to increase genome stabilization. SR proteins can control the concentrations of specific mRNA that is successfully translated into protein by selecting for nonsense-mediated decay codons during alternative splicing. SR proteins can alternatively splice NMD codons into its own mRNA transcript to auto-regulate the concentration of SR proteins. Through the mTOR pathway and interactions with polyribosomes, SR proteins can increase translation of mRNA.Ataxia telangiectasia, neurofibromatosis type 1, several cancers, HIV-1, and spinal muscular atrophy have all been linked to alternative splicing by SR proteins.