PRE-AP Stage 3 – Learning Plan
... DIFFERENTIATION SCAFFOLD: Students will observe the significance of genetic variation as a result of the possible outcomes of various genetic combinations. Students will also examine the different genetic techniques used in studying the genomes of organisms. ACCELERATE: PREP – dominant, recessive, g ...
... DIFFERENTIATION SCAFFOLD: Students will observe the significance of genetic variation as a result of the possible outcomes of various genetic combinations. Students will also examine the different genetic techniques used in studying the genomes of organisms. ACCELERATE: PREP – dominant, recessive, g ...
Human Metabolic Network Reconstruction
... What data is included in a reconstruction? Genome‐scale network reconstructions include a variety of biological data types (genomic, transcriptomic, proteomic, metabolomic, phenomic) that are manually collected from the literature and biological databases. Examples of how th ...
... What data is included in a reconstruction? Genome‐scale network reconstructions include a variety of biological data types (genomic, transcriptomic, proteomic, metabolomic, phenomic) that are manually collected from the literature and biological databases. Examples of how th ...
Opening for a PhD student Genomics of Migration
... technologies to identify the genetic basis of migratory traits. Here we focus on identifying the genes and signalling pathways behind the components shaping the migratory phenotype in the blackcap, a well characterized migratory songbird species. We will complement the sequencing approach with gene ...
... technologies to identify the genetic basis of migratory traits. Here we focus on identifying the genes and signalling pathways behind the components shaping the migratory phenotype in the blackcap, a well characterized migratory songbird species. We will complement the sequencing approach with gene ...
Document
... Shotgun-sequencing Method First proposed by Craig Venter, Hamilton Smith and Leroy Hood in 1996, focuses on the sequencing stage and then mapping., it starts with a BAC clone with very large inserts, averaging about 150 kb. The inserts in each BACs are sequenced on both ends using an automated seque ...
... Shotgun-sequencing Method First proposed by Craig Venter, Hamilton Smith and Leroy Hood in 1996, focuses on the sequencing stage and then mapping., it starts with a BAC clone with very large inserts, averaging about 150 kb. The inserts in each BACs are sequenced on both ends using an automated seque ...
Génmanipuláció
... negative selection marker (e.g. thymidine kinase, tk) is added to the replacement vector. The negative marker is outside the region of sequence similarity between the vector and the targeted locus. The engineered construct is added to cells which contain the targeted gene of interest. By mechanisms ...
... negative selection marker (e.g. thymidine kinase, tk) is added to the replacement vector. The negative marker is outside the region of sequence similarity between the vector and the targeted locus. The engineered construct is added to cells which contain the targeted gene of interest. By mechanisms ...
Open questions: A logic (or lack thereof) of genome organization COMMENT Open Access
... bringing combinations together or breaking them up? How often will selection care about single point mutations within microRNA pairing sites? Do genes evolve to avoid pairing with certain microRNAs [6]? The list of questions goes on (and should keep us in business for a while yet). The questions are ...
... bringing combinations together or breaking them up? How often will selection care about single point mutations within microRNA pairing sites? Do genes evolve to avoid pairing with certain microRNAs [6]? The list of questions goes on (and should keep us in business for a while yet). The questions are ...
Slide 1
... Population Stratification can be a problem • Imagine a sample of individuals drawn from a population consisting of two distinct subgroups which differ in allele frequency. • If the prevalence of disease is greater in one sub-population, then this group will be over-represented amongst the cases. • ...
... Population Stratification can be a problem • Imagine a sample of individuals drawn from a population consisting of two distinct subgroups which differ in allele frequency. • If the prevalence of disease is greater in one sub-population, then this group will be over-represented amongst the cases. • ...
Gilbert - C-MORE
... 1. How to acess the habitat specific gene pool information? Recommendation : Create a comprehensive portal that can store such datasets. 2. High-throughput methods to screen orthologous genes across multipule population genomes a. some methods exist, but they are specific for genome sequences of cul ...
... 1. How to acess the habitat specific gene pool information? Recommendation : Create a comprehensive portal that can store such datasets. 2. High-throughput methods to screen orthologous genes across multipule population genomes a. some methods exist, but they are specific for genome sequences of cul ...
TALK
... amount of DNA which serves no useful function for the cell. Introns, inteins, transposons and pesudogenes are examples of "selfish DNA", which persist because their impact on cellular replication efficiency is too small for selection to act directly. This DNA may be eliminated by chance due to a gen ...
... amount of DNA which serves no useful function for the cell. Introns, inteins, transposons and pesudogenes are examples of "selfish DNA", which persist because their impact on cellular replication efficiency is too small for selection to act directly. This DNA may be eliminated by chance due to a gen ...
Cancer Genetics
... is an alternatively spliced isoform of HDAC9 that does not contain the catalytic site40. HDACs and HATs determine access of the transcriptional machinery to DNA that is complexed to chromatin, by altering the structure of nucleosomes. HDACs, by decreasing the level of histone acetylation, can lead t ...
... is an alternatively spliced isoform of HDAC9 that does not contain the catalytic site40. HDACs and HATs determine access of the transcriptional machinery to DNA that is complexed to chromatin, by altering the structure of nucleosomes. HDACs, by decreasing the level of histone acetylation, can lead t ...
Chapter 16: Genome Analysis: DNA Typing, Genomics, and
... • This means that DNA sequence variants at many genetic loci influence the phenotype. ...
... • This means that DNA sequence variants at many genetic loci influence the phenotype. ...
comparative genomics
... Second, within a given species most individuals are genetically distinct in a number of ways. What does it actually mean, for example, to "sequence a human genome"? The genomes of two individuals who are genetically distinct ...
... Second, within a given species most individuals are genetically distinct in a number of ways. What does it actually mean, for example, to "sequence a human genome"? The genomes of two individuals who are genetically distinct ...
Illumina Solexa
... four separate fluorophores are all present and followed over time. Since it is the native polymerase (albeit modified), very long reads can be obtained but since it is a single molecule, error rates are ...
... four separate fluorophores are all present and followed over time. Since it is the native polymerase (albeit modified), very long reads can be obtained but since it is a single molecule, error rates are ...
Biological databases-Intro
... To connect knowledge to sequences we need automatic methods, workflows, text mining. Most of this is limited by close database systems. Only available is PubMed. But PubMed has only short abstracts. NO information about conditions, M&M etc We need to change this culture ...
... To connect knowledge to sequences we need automatic methods, workflows, text mining. Most of this is limited by close database systems. Only available is PubMed. But PubMed has only short abstracts. NO information about conditions, M&M etc We need to change this culture ...
Genetic Justice
... • 1953 James Watson and Francis Crick discover structure of DNA... • 2003: Human Genome Project completed • Dec. 2005: Cancer Genome Atlas • …number (and accuracy) of genetic tests is rapidly increasing…over 1000 tests • -Tests: PKU, Huntington’s, CF, Tay Sachs, Sickle-Cell… • -Goal of $1000 human g ...
... • 1953 James Watson and Francis Crick discover structure of DNA... • 2003: Human Genome Project completed • Dec. 2005: Cancer Genome Atlas • …number (and accuracy) of genetic tests is rapidly increasing…over 1000 tests • -Tests: PKU, Huntington’s, CF, Tay Sachs, Sickle-Cell… • -Goal of $1000 human g ...
Human Genome Project The Human Genome Project
... sizes, ranging from 2,000 to 300,000 base pairs in length, forming what is called a DNA "library". Using an automated DNA sequencer the DNA is read in 800bp lengths from both ends of each fragment. Using a complex genome assembly algorithm and a supercomputer, the pieces are combined and the genome ...
... sizes, ranging from 2,000 to 300,000 base pairs in length, forming what is called a DNA "library". Using an automated DNA sequencer the DNA is read in 800bp lengths from both ends of each fragment. Using a complex genome assembly algorithm and a supercomputer, the pieces are combined and the genome ...
Sequencing Requirements Requirements for DNA sequencing: Only
... 6. DNA concentrations are best estimated by comparison with the bands of a DNA mass ladder on an agarose gel or a NanoDrop spectrophotometer. a. Purified DNA should run as a single band on an agarose gel b. For NanoDrop, the A260/A280 ratio should be 1.8. The NanoDrop only requires 1-2 uL of sample ...
... 6. DNA concentrations are best estimated by comparison with the bands of a DNA mass ladder on an agarose gel or a NanoDrop spectrophotometer. a. Purified DNA should run as a single band on an agarose gel b. For NanoDrop, the A260/A280 ratio should be 1.8. The NanoDrop only requires 1-2 uL of sample ...
BB30055: Genes and genomes
... (IHGSC) - composite from several different people generated from 10-20 primary samples taken from numerous anonymous donors across racial and ethnic groups (B) Celera Genomics – 5 different donors (one of whom was J Craig Venter himself !!!) ...
... (IHGSC) - composite from several different people generated from 10-20 primary samples taken from numerous anonymous donors across racial and ethnic groups (B) Celera Genomics – 5 different donors (one of whom was J Craig Venter himself !!!) ...
Researchers Scrutinize Brown Tide Genes (pdf)
... Dr. Astrid Terry, a genome analyst from the Joint Genome Institute in Walnut Creek, CA, described how the Aureococcus genome with its 11,500 genes has a gene count larger than all eukaryotic algae sequenced to date and the nuclear genome of Aureococcus is surprisingly most similar to that of another ...
... Dr. Astrid Terry, a genome analyst from the Joint Genome Institute in Walnut Creek, CA, described how the Aureococcus genome with its 11,500 genes has a gene count larger than all eukaryotic algae sequenced to date and the nuclear genome of Aureococcus is surprisingly most similar to that of another ...
locomotion in a consanguineous kindred the gene
... The biological basis for the development of the cerebro-cerebellar structures required for posture and gait in humans is poorly understood. We investigated a large consanguineous family from Turkey exhibiting an extremely rare phenotype associated with quadrupedal locomotion, mental retardation, and ...
... The biological basis for the development of the cerebro-cerebellar structures required for posture and gait in humans is poorly understood. We investigated a large consanguineous family from Turkey exhibiting an extremely rare phenotype associated with quadrupedal locomotion, mental retardation, and ...
HGP102new
... What does the draft human genome sequence tell us? By the Numbers • The human genome contains 3164.7 million chemical nucleotide bases (A, C, T, and G). • The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. • The ...
... What does the draft human genome sequence tell us? By the Numbers • The human genome contains 3164.7 million chemical nucleotide bases (A, C, T, and G). • The average gene consists of 3000 bases, but sizes vary greatly, with the largest known human gene being dystrophin at 2.4 million bases. • The ...
The BCM Microarray Core Facility
... The combination of read-length and paired-end flexibility allows for high quality genomic sequencing applications. Illumina’s sequencing chemistry supports 100+ bp single-reads, short- and long-insert paired-end reads for whole-genome sequencing and resequencing, SNP discovery, identification of cop ...
... The combination of read-length and paired-end flexibility allows for high quality genomic sequencing applications. Illumina’s sequencing chemistry supports 100+ bp single-reads, short- and long-insert paired-end reads for whole-genome sequencing and resequencing, SNP discovery, identification of cop ...
Neuroscience Gene Vector and Virus Core
... Production scale desired (i.e., number and size of flasks of cells to be transfected to produce the virus? Transfection of two to five T-225s is often used to produce AAV. However scales ranging from one well of a 96 well plate to 100 T-225s are possible. Transfection of one to two T-225s are the mi ...
... Production scale desired (i.e., number and size of flasks of cells to be transfected to produce the virus? Transfection of two to five T-225s is often used to produce AAV. However scales ranging from one well of a 96 well plate to 100 T-225s are possible. Transfection of one to two T-225s are the mi ...
presentation - Genome-to-Genome Distance Calculator
... Several methods to obtain HSPs have been described Blast (Altschul et al. 1990): probably most frequently ...
... Several methods to obtain HSPs have been described Blast (Altschul et al. 1990): probably most frequently ...
Whole genome sequencing
Whole genome sequencing (also known as full genome sequencing, complete genome sequencing, or entire genome sequencing) is a laboratory process that determines the complete DNA sequence of an organism's genome at a single time. This entails sequencing all of an organism's chromosomal DNA as well as DNA contained in the mitochondria and, for plants, in the chloroplast.Whole genome sequencing should not be confused with DNA profiling, which only determines the likelihood that genetic material came from a particular individual or group, and does not contain additional information on genetic relationships, origin or susceptibility to specific diseases. Also unlike full genome sequencing, SNP genotyping covers less than 0.1% of the genome. Almost all truly complete genomes are of microbes; the term ""full genome"" is thus sometimes used loosely to mean ""greater than 95%"". The remainder of this article focuses on nearly complete human genomes.High-throughput genome sequencing technologies have largely been used as a research tool and are currently being introduced in the clinics. In the future of personalized medicine, whole genome sequence data will be an important tool to guide therapeutic intervention. The tool of gene sequencing at SNP level is also used to pinpoint functional variants from association studies and improve the knowledge available to researchers interested in evolutionary biology, and hence may lay the foundation for predicting disease susceptibility and drug response.