Download Supplemental Data Figure 1

Supplemental Data Figure 1. 5’, central or 3’ location of the regions selected for the
amplification (……… ) along the coding regions (______) of transcript analysed using
primers detailed in Table 1. Information on size of full-length of coding regions and size
of amplified region only [ ] are illustrated for all gene products analysed
Supplemental Data Figure 2. Ins I mRNA analysis of MIN6 B1 cells, grown at a range
of densities, and their corresponding CM. RT-VE=reaction with H2O instead of RNA as
control; PCR-VE=PCR reaction with H2O instead of cDNA as control. M=molecular
weight marker: X174 DNA HaeIII digest. Results are representative of n=3 biological
repeats.
Supplemental Data Figure 3. Human preproinsulin mRNA analysis of Vero-PPI cells,
grown at a range of densities, and their corresponding CM. RT-VE=reaction with H2O
instead of RNA as control; PCR-VE=PCR reaction with H2O instead of cDNA as a
control. M=molecular weight marker: X174 DNA HaeIII digest. Results are
representative of n=3 biological repeats.