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+ What occurs during the M phase? Sections 8.6 and 8.7 + Mitosis and Cytokinesis + Prophase (including prometaphase)  Nuclear membrane breaks down  Chromatin condenses into chromosomes  Spindle fibers appear  Centriole + spindle fibers = centrosome  Centrosomes move to poles  Nucleolus disappears  Kinetochore of centromere on each sister chromatid attaches to spindle fiber + Prophase… + Metaphase  Spindle is fully formed  Chromosomes line up in the “middle” of the cell (at the metaphase plate – which is between the poles of the cell) + Anaphase  Nonkinetochore microtubules elongate the cell  Centromeres of sister chromatids split  Motor proteins of kinetochores pull daughter chromosomes to the poles centromere 1st along microtubules  Ends when chromosomes reach the poles + Telophase  Nuclear membranes reappear (one around each set of chromosomes)  Chromosomes uncoil to chromatin  Nucleolus reappears  Spindle breaks down  Cytokinesis begins during this stage + Cytokinesis  Division of cytoplasms  Starts during telophase  Animal Cells – pinching inward forms a cleavage furrow  Plant cells – vesicles align between the two nuclei; vesicles fuse to form a cell plate; cell plate grows outward