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Viruses & Bacteria Chapter 19 Pages 471 - 490 Compare/Contrast Prokaryotes (Bacteria) Eukaryotes Smaller Simpler Has genetic material No nucleus Grow, reproduce, & respond to environment Some move by gliding or swimming Some have internal membranes Unicellular Larger Complex Has genetic material Nucleus Grow, reproduce, & respond to environment All move Have internal membranes Unicellular & multicellular Has organelles & cytoplasm Bacteria • Prokaryotes –Smallest, most common microorganism –Single-celled –Lack nucleus –Has DNA Bacteria • 2 Kingdoms • Eubacteria (pg. 472) – live almost everywhere, larger of the two. – Usually surrounded by a cell wall made up of carbohydrate & peptidoglycan. – Cell membrane inside cell wall. – Cytoplasm. – Ex. E coli Bacteria • 2 Kingdoms • Archaebacteria – Look similar. – Live in harsh environments. – Lack peptidoglycan in the cell wall. – Have different membrane lipids. – DNA sequences of key genes are more like those of eukaryotes than eubacteria. – Are thought to be ancestors of eukaryotes. – Ex. Methangones- produce methane gas. Bacteria • How we Identify Prokaryotes: • Shape – bacilli, cocci, spirilla. • By the way they move. • By the way they obtain energy. – Most are Heterotrophes which obtain energy by consuming food. » Chemoheterotroph- Ex. Botulism (food poisoning). » Photoheterotroph- uses photosynthesis. – Autotrophs make their own food. » Chemoautotroph- found in deep ocean floors. » Photoautotroph- found near surface in lakes oceans & streams. • The chemical nature of their cell walls. – A method of Gram Staining is used to determine nature. Gram (+) = violet, Gram (-) = pink. Bacteria • How we Identify Prokaryotes (cont.): – How they release energy by cellular respiration and fermentation • Obligate aerobes – require constant supply of oxygen. Ex. Tuberculosis. • Obligate anaerobes – do not require oxygen. Oxygen could kill it. Ex. Botulism is found in canned food that’s not properly sterilized. • Facultative anaerobes – can survive with or without oxygen. Can live anywhere. Ex. E coli which is found in the large intestines or in sewage or contaminated water. Bacteria • Reproduction – Binary fission – DNA replicates & divides producing 2 daughter cells. Asexual reproduction. – Conjugation – process where genetic information is exchanged. – Spore formation – an endospore may form when growth conditions become unfavorable. Bacteria • Importance of Bacteria: – Producers – that carry out photosynthesis. – Decomposers – that break down dead matter “natural recycler”. – Nitrogen fixers – process in which soil bacteria convert natural nitrogen gas into a form plants can use. – Human use – industry (clean up oil spills), food production, synthesize drugs, mine minerals, & remove waste products & poisons from water. • Ex. E coli in intestines make vitamins that the body cannot produce themselves which is a symbiotic relationship (both benefit). Viruses • Viruses are particles of: – Nucleic acid – Protein – Lipids (only in some) • They vary in size and structure. • They enter living cels & use the machinery of the infected cell to produce more viruses. • Composed of: – A core DNA or RNA (which has the instructions for making copies) – This is surrounded by a protein coat called a capsid which binds the virus to the surface of the host cell. Viruses • Bacteriophages– Viruses that infect bacteria. – Ex. Bacteriophage T4 Viruses • Once inside a host cell: pg. 481 – Lytic infection • Virus inters cell and makes copies of itself. • Causes cell to burst, releasing new virus particales that can attack other cells. • It then uses materials of host cell to make copies of its own DNA molecule. • Host cell is destroyed. – Lysogenic infection • Virus integrates its DNA into the DNA of host cell. • The viral genetic info. replicates along with the host cell’s DNA. • Viral DNA that’s embedded in host’s DNA is called prophage. • Unlike lytic, it does not lyse the host cell right away so it may remain a part of DNA of host for many generations. Viruses Retroviruses – contain RNA as their genetic information. – Genetic information is copied backwards. – It may remain dormant for any length of time before becomes active and it can cause death of the host cell. – Ex. AIDS, some cancers. Viruses • Must infect living cells in order to reproduce. • Are Parasites. • Are not considered to be living things because they are not made up of cells & cannot live independently. • See Chart on pg. 483 Diseases Caused by Bacteria & Viruses • Pathogens – Disease- causing agents. – Include bacteria & viruses that cause disease (not all bacteria). – Some live in and on the human body & help it perform essential functions – Chart on pg. 486 Bacterial Diseases • Louis Pasteur – the first to show that bacteria caused diseases and established the germ theory of disease. • Diseases: – Tuberculosis- bacteria is inhaled into lungs which destroys tissue and gets into the blood to travel to other places throughout the body. – Strep Throat- bacteria releases toxins. This bacteria also causes scarlet fever. – Diphtheria- infects tissues of the throat which leads to breathing problems, heart failure, paralysis & death. – Lyme Disease – Tooth Decay Bacteria produce disease in 2 ways: • Bacteria damage the cells & tissues of the infected organism directly by breaking down the cells for food. • Bacteria release toxins (poisons) that travel throughout the body interfering with the normal activity of the host. Prevention & Control • Vaccine – a preparation of weakend or killed pathogens. It can prompt the body to produce immunity to the disease. • Immunity – the body’s natural way of killing pathogens. • Antibodies – compounds that block the growth & reproduction of bacteria. – When a bacterial infection occurs, antibiotics can be used to fight the disease. – Animals can suffer from bacterial diseases as well. Ex. Anthrax – sheep to farmers & wool workers which can lead to death. (biological warfare) Viral Diseases • Viruses disrupt the body’s normal equilibrium. • Viruses attack & destroy certain body cells causing the symptoms of the disease. • Diseases: Chart pg. 489 – – – – – Common cold Influenza AIDS Chickenpox Measles • Can produce other serious diseases in both animals & plants. • Plant viruses have a difficult time entering cells they infect partly because plant cells have though cell walls. Viral Diseases • 2 Virus like particles can cause disease: • Viroids – single-stranded RNA molecules that have no surrounding capsoid. (cause disease in plants). – Ex. Tobacco mosaic virus. • Prions – particles that contain only protein which have no DNA or RNA. (cause disease in animals & humans). – Ex. Foot & mouth disease in livestock. Protection Against • Vaccines – prevention. • Antibiotics – viruses do not respond to antibiotics but sometime the symptoms can be treated. Prevention & Control • Methods of controlling bacterial growth: – Sterilization – heat; most can’t survive high temperatures for long period on time.(kills bacteria) – Disinfectants – soaps & cleaning solutions; sterilizes hospitals; overuse may cause bacteria to evolve.(kills bacteria) – Food storage – refrigeration (bacteria takes longer to multiply at low temperatures); boiling; frying; steaming. – Food processing – canning (heated to high temperature, placed into sterile jars & sealed); chemical treatments such as salt, vinegar,sugar. (kills bacteria)