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Transcript 
Load organisms from green house.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Click “open”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Select an organism for self cross
for eg. The Red organism
Then click tab
“Self-cross One Organism”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Resulting offsprings of Red organism.
Then double click on Tray1
and click “Send to Lower Panel”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Select two different organisms(heterozygous)
Then click the tab
“Cross Two Organisms”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Resulting offsprings of Red and Green-1
organism.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Select two same organisms(homozygous)
Then click the tab
“Cross two Organisms”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Resulting offsprings of Red
organism.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Select an organism
Then click
“Mutate One
Organism”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Mutant versions of Green-2 organism.
Then click the tab
“Biochemistry”.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
In the Biochemistry add
amino acid sequence
SSSSSSS here.
History List is populated
with corresponding
template and
phenotype.-White
organism
Then click
“FOLD”
The default
phenotype
Corresponding phenotype populated
when sequence is folded.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Enter another
sequence
FFFFFFF
and click “FOLD”
Heterozygous combined
phenotype(Red)
Corresponding
phenotype(Red)
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Enter the same sequence
FFFFFFF here and click “FOLD”
Homozygous combined
phenotype.(Red )
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Then click tab
“Molecular Biology”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Click the tab
“Enter New DNA”
Then enter the DNA sequenceTATAAATGTCTAATCGTCATATTTT
ATTAGTTTTTTGTCGTCAATAGGGGGG
and click “OK”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Then click
“Fold Protein”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Resulting Organism -Red
Default Phenotype
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Double click “History List”
and select “Send to Lower
Panel”
or
Enter the same DNA by
clicking “Enter New DNA”
in Lower panel
.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
The same two DNA sequences
give the combined phenotype
here(Red) which is
homozygous.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Enter DNACAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTG
CGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT
and click “OK”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Click “Fold Protein”
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
Resulting Green-1 organism
The two different DNA
sequences gives a
Heterozygous combined
phenotype which populates
here.
24 April, 2008
MGX TUTORIAL
MGX © 2007-2008
UMASS BOSTON
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