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How long have there been cells? •Bacteria evolved about 3 billion years ago. • Can be just a simple membrane filled with DNA. What allowed us to see cells? The Microscope Robert Hooke Cells The basic unit of life. The building blocks of all living things The Cell Theory All living things are made of cells. Cells come from other cells (Cell Division). Cells are the smallest working units of living things. Levels of Organization Smallest is a cell tissue organ Organ system Largest is an organism Prokaryotes A prokaryotic cell has no “true nucleus” and lack membrane bound organelles. Still make proteins and have DNA Eukaryotes Eukaryotes have a nucleus and also have all of the membrane bound organelles. Plant Cell Key Feature? Tough, rigid CELL WALL Animal Cell Cytoplasm Cytoplasm Cytoplasm The “juice” of the cell. The “right” environment for the chemical reactions of the cell. Lipid Bilayers Cell Membrane Cell Membrane, a lipid bilayer The barrier between the cell and the environment. Controls what gets in and out of a cell. Building different membranes is one way different cell types are made. Plant Cell Cell Wall Cell Wall Made of cellulose. Serves to give a firm structure to plants Can’t poop? Eat more fiber (cellulose) Nuclear Membrane Membrane around nucleus. Carefully controls what gets in and out. Protects DNA! Nucleus Nucleus Nucleus Membrane Bound* Contains DNA (the genetic instructions that make you… you). Nucleolus (Be careful to spell correctly) Ribosomes (RNA and proteins) assembled here Endoplasmic Reticulum (ER) ER Endoplasmic Reticulum, the ER Membrane Bound* Smooth ER makes lipids. (For Membranes) Rough ER (ribosomes) makes proteins. Sends proteins to Golgi. Golgi Body (Golgi Stack) Golgi Golgi Body Sorts proteins. Sent to: Secreted (released from cell) Plasma Membrane Membrane bound organelles. Secretes INSULIN. Pancreas? Not working? Diabetes. Lysosome Lysosome Lysosomes and peroxisomes Membrane Bound* Store or destroy waste. Lysosomes are acidic, like your stomach. Secretory Pathway Proteins are coded for by DNAmade on rough ER by ribosomessent to Golgi for sortinggo to Lysosome Nucleus Cell Membrane or release out of cell. Vacuole Vacuole Vacuole Large in plants. Stores water Chloroplasts Chloroplast Chloroplasts Membrane Bound* Sunlight energy is converted to sugar. Filled with chlorophyll, making plants GREEN! Mitochondria Mitochondria Mitochondria Membrane Bound* (double membrane) Sugar is BURNED, making ATP (Energy). PLANTS and ANIMALS get their ENERGY HERE!! Centrioles Centrioles Centrioles During Cell Division separate chromosomes into the daughter cells. Plants organize their spindles, but lack centrioles. Cytoskeleton Cytoskeleton Cytoskeleton Supports the cell: Actin Microtubules Molecules are also transported through the cytosol along these skeletal “tracks” with tiny motors. Chromosomes DNA, instructions for making proteins Proteins manage the chemical reactions that are YOU! Plant or Animal Cell? Ribosomes Make proteins In cytoplasm or studding the surface of the rough ER. Plant Cells Chloroplasts Cell Wall Larger Vacuole Animal Cells Centrioles Composition of Cell Membrane Proteins Carbohydrates Phosphate heads Lipid tails Phospholipid Bilayer Diffusion Movement of molecules from a high concentration to a low concentration When molecules spread out to an even concentration Concentration Gradient Concentration difference on two sides of a barrier High Low Equilibrium An even concentration on both sides of the membrane Examples Perfume Gas Dye in water Ultimately the molecules want to create a balance or EQUILIBRIUM Try This In a diffusion situation: Osmosis Movement of water from a high to a low concentration, in an attempt to even out a concentration gradient of solute particles. Examples Water in a dam Waterfalls Wants to create an EQUILIBRIUM Solve: 100 % Water 90% H2O 10 % Iodine 95 % H2O 5% Iodine 95% H2O 5 % Iodine Plasmolysis A special case involving osmosis and plants When water is drawn out of the cell causing the cell membrane to shrink. Selectively Permeable http://www.indiana.ed u/~p hys215/lecture/lec notes/lecgraphics/ diffusion2.gif Passive transport – takes NO energy. (like flowing downhill) Only works from High to Low concentrations! Channels – only let certain things through Active transport – Takes energy! (like walking uphill) proteins- Use energy (ATP) to move substances channels – May transport two molecules one is being concentrated while other is diffusing (flowing from High to Low) http://www.biologie.uni-hamburg.de/bonline/library/biology107/bi107vc/fa99/terry/images/ATPpum A.gif Endocytosis (UPTAKE) A “bubble” of membrane pinches off Moving large particles INTO the cell. Exocytosis (secretion) – think opposite of endocytosis (like EXIT) A “bubble” of membrane fuses with the cell membrane Large particles move OUT of the cell. Inside Outside Phagocytosis - like endocytosis but with very large particles (bacteria) Red Blood Cells being “eaten” by a disease fighting White Blood Cell http://gak.med.kagawa-u.ac.jp/~daigin/eng_info/Hisology_&_Cell_Biology/PIC2.jpg Tissues What makes skin and muscle cells different? Skin and muscle cells make different proteins, allowing different jobs to be done! What makes cells different? Even though each cell has all your DNA, different parts are “read” and used in different cells. This is called gene “expression” or gene use Muscle Tissue (myocytes) Cell Job: Movement Cell parts: cytoskeleton, myosin, and ATP (energy) Many cells fuse to form one long cell, with many nuclei Actin, Myosin and other proteins burn ATP to allow movement. Myosin walks on Actin “tracks” http://www.sci.sdsu.edu/movies/actin.gif Muscle Fibers shorten Nervous Tissue (neurons) Cell Job: Relay messages through the body Cell Parts needed: various channels to relay “electrical” signal Chemicals to send message cell to cell (neurotransmitters like dopamine, GABA, serotonin, acetylcholine) http://en.wikipedia.org/wiki/Image:SynapseIllustration.png Intestinal Tissue (epithelial cells) Cell Job: to absorb nutrients Cell Parts: Channels to let nutrients into cell Channels to pump nutrients into blood stream Concentrating nutrients in blood Eyes (Retinal Tissue) Cell Job: to sense light Cell Parts: various proteins that react to light and others to deliver the message to the optic nerve Rod cells in eye sense black and white Cones sense colors