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Sharp tools for sharp minds MACS® Products for tumor immunology research The immune system plays a crucial role in host protection against carcinogenesis by eliminating tumor cells. However, some of the tumor cells are not detected by the immune system and a variety of immunologic mechanisms contribute to tumor escape and subsequent tumor growth and angiogenesis. Miltenyi Biotec brings you cutting-edge solutions for your tumor immunology research—from time-saving, automated sample preparation through fast, efficient cell separation to sophisticated flow cytometric analysis. This poster shows a selection of Miltenyi Biotec products for cell separation and analysis designed to help you enhance your tumor immunology research. Scan here for more information. To download a reference list containing a multitude of articles on tumor immunology research using Miltenyi Biotec products go to www.miltenyibiotec.com/ti-references Tumor escape Tissue dendritic cell CD1a MicroBeads, human # 130-051-001 CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, human # 130-090-506 CD303 (BDCA-2) MicroBead Kit, human # 130-090-509 Anti-BDCA-1, -2, -3, and CD304 (BDCA-4/Neuropilin-1) antibodies, human CD11c MicroBeads, mouse # 130-052-001 CD209 (DC-SIGN) MicroBead Kit, human # 130-092-868 Dendritic cell CD11c MicroBeads, mouse # 130-052-001 Anti-mPDCA-1 MicroBeads, mouse # 130-091-965 Growing tumor* CSC CD133 MicroBead Kit, human # 130-050-801 CD133 antibodies, human CD44 MicroBeads, human # 130-095-194 CD44 antibodies, human CD24 MicroBead Kit, human # 130-095-951 CD24 antibodies, human Tumor antigen Dendritic cell Dendritic cell Lymphoid organ* IL-10 TGF-β NK cell CD56 MicroBeads, human # 130-050-401 NK Cell Isolation Kit, human # 130-092-657 NK Cell Isolation Kit, mouse # 130-090-864 KIR Typing Kit, human # 130-092-551 NK cell–specific antibodies CSC IL-6** IL-10 Macrophage CD11b MicroBeads, human and mouse # 130-049-601 CD11b antibodies, human and mouse IL-4 MDSC NK cell Macrophage Treg cell TGF-β IL-10 IL-10 TGF-β Naive CD4+ T cell Th2 cell Myeloid-Derived Suppressor Cell Isolation Kit, mouse # 130-094-538 CD11b antibodies, human and mouse Anti-Gr-1 antibodies, mouse Anti-Ly-6C antibodies, mouse CD8+ T cell MDSC iNKT cell CD8+ T cell IL-4** IL-10 IL-13 TGF-β Monocyte iNKT cell Anti-iNKT MicroBeads, human # 130-094-842 Anti-iNKT (6B11) antibodies, human IL-4 IL-5 IL-10 Th2 cell IL-13 Dendritic cell CD8+ T cell Treg cell B cell B cell Blood Cell Isolation Kit dendritic cell B(B-CLL), human Circulating tumor cell CD141 (BDCA-3) MicroBead Kit, human # 130-090-512 Ova Antigen Delivery Reagent # 130-094-663 Circulating tumor cell CD326 (EpCAM) MicroBeads, human # 130-061-101 Anti-ErbB-2 MicroBeads, human # 130-090-482 Carcinoma Cell Enrichment Kit # 130-060-101 Differentiation Relocation Naive CD4+ T Cell Isolation Kit II, human # 130-094-131 CD4+CD62L+ T Cell Isolation Kit II, mouse # 130-093-227 CD4+ Recent Thymic Emigrant Isolation Kit, human # 130-094-299 T cell–specific antibodies*** TGF-β** IL-2 Macrophage MDSC Pan DC MicroBeads, mouse # 130-092-465 CD11c antibodies, mouse Anti-mPDCA-1 MicroBeads, mouse # 130-091-965 Anti-mPDCA-1 antibodies, mouse Anti-Siglec-H antibodies, mouse CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, human # 130-090-506 CD303 (BDCA-2) MicroBead Kit, human # 130-090-509 CD303 (BDCA-2) antibodies, human CD209 (DC-SIGN) MicroBead Kit, human # 130-092-868 CD8+ Dendritic Cell Isolation Kit, mouse # 130-091-169 Naive CD4+ T cell Dendritic cell Treg cell Lymphoid organ dendritic cell Inhibition MDSC: Myeloid-derived suppressor cell CSC: Cancer stem cell # 130-093-660 B Cell Isolation Kit II, human # 130-091-151 CD138 MicroBeads, human # 130-051-301 B Cell Isolation Kit, mouse # 130-090-862 Whole Blood CD19 MicroBeads, human # 130-090-880 * Visit www.gentlemacs.com for specific protocols on tissue dissociation. Th2 cell Treg cell CD8+ T cell CD294 (CRTH2) MicroBead Kit, human # 130-091-274 IL-4 Secretion Assay – Cell Enrichment and Detection Kit, human # 130-054-101 Mouse IL-4 Secretion Assay – Cell Enrichment and Detection Kit # 130-090-515 IL-10 Secretion Assay – Cell Enrichment and Detection Kit (PE), human # 130-090-435 Mouse IL-10 Secretion Assay – Cell Enrichment and Detection Kit (PE) # 130-090-490 IL-13 Secretion Assay – Cell Enrichment and Detection Kit (PE), human # 130-093-480 CD4+ CD25+ Regulatory T Cell Isolation Kit, human # 130-091-301 CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit II, human # 130-094-775 CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit, human # 130-093-631 CD4+CD49d– Regulatory T Cell Isolation Kit, human # 130-094-551 CD4+ CD25+ Regulatory T Cell Isolation Kit, mouse # 130-091-041 CD127 MicroBead Kit, human # 130-094-945 CD8 MicroBeads, human # 130-045-201 CD8+ T Cell Isolation Kit, human # 130-094-156 CD8+CD45RA+ Effector T Cell Isolation Kit, human # 130-094-485 CD8 (Ly-2) MicroBeads, mouse # 130-049-401 CD8a+ T Cell Isolation Kit II, mouse # 130-095-236 Whole Blood CD8 MicroBeads, human # 130-090-878 ** Visit www.miltenyibiotec.com/cytokines for the complete cytokine portfolio. *** Visit www.miltenyibiotec.com for a complete list of antibodies. Miltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 macs@miltenyibiotec.de Miltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602-9562, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 530 888 8925 macs@miltenyibiotec.com Miltenyi Biotec Australia Pty. Ltd. Phone +61 2 8877 7400 macs@miltenyibiotec.com.au Miltenyi Biotec B.V. (Benelux) macs@miltenyibiotec.nl Customer service Netherlands Phone 0800 4020120 Customer service Belgium Phone 0800 94016 Customer service Luxembourg Phone 800 24971 Miltenyi Biotec Trading (Shanghai) Co., Ltd. (P.R. China) Phone +86 21 6235 1005 macs@miltenyibiotec.com.cn Miltenyi Biotec SAS (France) Phone +33 1 56 98 16 16 macs@miltenyibiotec.fr Miltenyi Biotec S.r.l. (Italy) Phone +39 051 646 0411 macs@miltenyibiotec.it Miltenyi Biotec K.K. (Japan) Phone +81 3 5646 8910 macs@miltenyibiotec.jp MACS is a registered trademark of Miltenyi Biotec GmbH. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. Copyright © 2011 Miltenyi Biotec GmbH. All rights reserved. Miltenyi Biotec Asia Pacific Pte. Ltd. (Singapore) Phone +65 6238 8183 macs@miltenyibiotec.com.sg Miltenyi Biotec S.L. (Spain) Phone +34 1 512 12 90 macs@miltenyibiotec.es Miltenyi Biotec Ltd. (UK) Phone +44 1483 799 800 macs@miltenyibiotec.co.uk miltenyibiotec.com 130-096-071 Miltenyi Biotec: Your first choice for sample preparation, cell separation, cell analysis, molecular analysis Sharp tools for sharp minds MACS® Products for tumor immunology research The immune system plays a crucial role in host protection against carcinogenesis by eliminating tumor cells. However, some of the tumor cells are not detected by the immune system and a variety of immunologic mechanisms contribute to tumor escape and subsequent tumor growth and angiogenesis. Miltenyi Biotec brings you cutting-edge solutions for your tumor immunology research—from time-saving, automated sample preparation through fast, efficient cell separation to sophisticated flow cytometric analysis. This poster shows a selection of Miltenyi Biotec products for cell separation and analysis designed to help you enhance your tumor immunology research. Scan here for more information. To download a reference list containing a multitude of articles on tumor immunology research using Miltenyi Biotec products go to www.miltenyibiotec.com/ti-references Tumor elimination Tissue dendritic cell CD1a MicroBeads, human # 130-051-001 CD1c (BDCA-1)+ Dendritic Cell Isolation Kit, human # 130-090-506 CD303 (BDCA-2) MicroBead Kit, human # 130-090-509 Anti-BDCA-1, -2, -3, and CD304 (BDCA-4/Neuropilin-1) antibodies, human CD11c MicroBeads, mouse # 130-052-001 CD209 (DC-SIGN) MicroBead Kit, human # 130-092-868 NK cell CD56 MicroBeads, human # 130-050-401 NK Cell Isolation Kit, human # 130-092-657 NK Cell Activation / Expansion Kit, human # 130-094-483 KIR Typing Kit, human # 130-092-551 KIR-specific antibodies NK Cell Isolation Kit, mouse # 130-090-864 Tumor antigen Regressing tumor* Dendritic cell Lymphoid organ* Dendritic cell IL-12 iNKT cell IL-12** TNF-α IL-6 IL-8 Macrophage CD11b MicroBeads, human and mouse # 130-049-601 Anti-mPDCA-1 MicroBeads, mouse # 130-091-965 CD4+ Dendritic Cell Isolation Kit, mouse # 130-091-262 CD8+ Dendritic Cell Isolation Kit, mouse # 130-091-169 Pan DC MicroBeads, mouse # 130-092-465 CD303 (BDCA-2) MicroBead Kit, human # 130-090-509 Plasmacytoid Dendritic Cell Isolation Kit II, mouse # 130-092-786 IL-12 Secretion Assay – Cell Enrichment and Detection Kit (PE), human # 130-092-122 Naive CD4+ T cell iNKT cell Anti-iNKT MicroBeads, human # 130-094-842 Anti-iNKT (6B11) antibodies, human IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human # 130-090-488 Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) # 130-090-517 Lymphoid organ dendritic cell Naive CD4+ T cell Naive CD4+ T Cell Isolation Kit II, human # 130-094-131 CD4+CD62L+ T Cell Isolation Kit II, mouse # 130-093-227 CD4+ Recent Thymic Emigrant Isolation Kit, human # 130-094-299 T cell–specific antibodies*** IL-12** IFN-γ CD8+ T cell IFN-γ Th1 cell NK cell Macrophage CD8+ T cell Naive CD8 T Cell Isolation Kit, human # 130-093-244 CD8+ Memory T Cell Isolation Kit, human # 130-094-412 + IFN-γ IL-2** IFN-γ CD8+ T cell Th1 cell Th1 cell CD8+ T cell Th1 cell IFN-γ Monocyte Blood Monocyte Th1 cell CD8+ T cell PepTivator® Peptide MicroBeads, CD8 MicroBeads, IL-2 Secretion dendritic cell CD14 Pools related to human human Assay – Cell Enrichment and # 130-050-201 # 130-045-201 Blood Dendritic Cell Detection Kit Monocyte Isolation CD8 T Cell Isolation tumor immunology Isolation Kit II, human (PE), human Kit II, human Kit, human Dendritic cell + # 130-091-379 CD304 (BDCA-4/Neuropilin-1) MicroBead Kit, human # 130-090-532 Diamond Plasmacytoid Dendritic Cell Isolation Kit, human # 130-092-402 Plasmacytoid Dendritic Cell Isolation Kit, human # 130-092-207 Myeloid Dendritic Cell Isolation Kit, human # 130-094-487 IFN-α Secretion Assay – Detection Kit (PE), human # 130-094-161 Differentiation # 130-091-153 Mo-DC Generation Toolbox, human # 130-093-568 Mo-DC Differentiation Inspector, human # 130-093-567 Whole Blood CD14 MicroBeads, human # 130-090-879 Relocation # 130-090-488 Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE) # 130-090-492 IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE), human # 130-054-201 Mouse IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) # 130-090-517 Memory CD4+ T Cell Isolation Kit, human # 130-091-893 CD4+ Effector Memory T Cell Isolation Kit, human # 130-094-125 CD4+ Central Memory T Cell Isolation Kit, human # 130-094-302 PepTivator Survivin 1, human PepTivator MAGE-A1, human PepTivator MAGE-A3, human PepTivator MAGE-A4, human PepTivator NY-ESO-1, human PepTivator Tyrosinase, human PepTivator gp100/pmel 17, human PepTivator Melan-A/MART-1, human # 130-094-156 CD8+CD45RA+ Effector T Cell Isolation Kit, human # 130-094-485 CD8 (Ly-2) MicroBeads, mouse # 130-049-401 CD8a+ T Cell Isolation Kit II, mouse # 130-095-236 For more information visit www.miltenyibiotec.com/peptivator *Visit www.gentlemacs.com for specific protocols on tissue dissociation. **Visit www.miltenyibiotec.com/cytokines for the complete cytokine portfolio. ***Visit www.miltenyibiotec.com for a complete list of antibodies. Miltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 macs@miltenyibiotec.de Miltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602-9562, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 530 888 8925 macs@miltenyibiotec.com Miltenyi Biotec Australia Pty. Ltd. Phone +61 2 8877 7400 macs@miltenyibiotec.com.au Miltenyi Biotec B.V. (Benelux) macs@miltenyibiotec.nl Customer service Netherlands Phone 0800 4020120 Customer service Belgium Phone 0800 94016 Customer service Luxembourg Phone 800 24971 Miltenyi Biotec Trading (Shanghai) Co., Ltd. (P.R. China) Phone +86 21 6235 1005 macs@miltenyibiotec.com.cn Miltenyi Biotec SAS (France) Phone +33 1 56 98 16 16 macs@miltenyibiotec.fr Miltenyi Biotec S.r.l. (Italy) Phone +39 051 646 0411 macs@miltenyibiotec.it Miltenyi Biotec K.K. (Japan) Phone +81 3 5646 8910 macs@miltenyibiotec.jp MACS is a registered trademark of Miltenyi Biotec GmbH. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. Copyright © 2011 Miltenyi Biotec GmbH. All rights reserved. Miltenyi Biotec Asia Pacific Pte. Ltd. (Singapore) Phone +65 6238 8183 macs@miltenyibiotec.com.sg Miltenyi Biotec S.L. (Spain) Phone +34 1 512 12 90 macs@miltenyibiotec.es Miltenyi Biotec Ltd. (UK) Phone +44 1483 799 800 macs@miltenyibiotec.co.uk miltenyibiotec.com 130-096-071 Miltenyi Biotec: Your first choice for sample preparation, cell separation, cell analysis, molecular analysis