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THE PEPPERED MOTH BISTON BETULARIA BETULARIA CARBONARIA Natural selection Microevolution: change in allele frequency in a population  Industrial Revolution caused tree trunks to be black from soot  By 1895, 98% of population of Peppered Moths near Manchester were black  Differential bird predation is thought to be the agent responsible  1950s: Kettlewell’s predation and mark/release/recapture experiments gave positive results. Following anti-pollution laws, carbonaria (the black variety) began to decline on both sides of the Atlantic  This was because the tree trunks were now paler and the black moth was easier to see  The pale moth numbers now increased  The agent of natural selection was the colour of the tree bark  As this changed from white to black and back again, the black and white varieties became more or less obvious.  BIOLOGICAL EVOLUTION  Defined as changes in heritable material (i.e. genes) through time.  Changes in the frequency of the carbonaria gene provides irrefutable proof of biological evolution KETTLEWELL’S BARREL EXPERIMENTS Black f. betularia f. carbonaria Totals 20 38 58 39 21 60 59 59 118 background White background Totals