Download DNA Structure and Analysis

Chapter 10 Lecture
Concepts of Genetics
Tenth Edition
DNA Structure and
Analysis
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10.1 The Genetic Material Must Exhibit
Four Characteristics
Replication
Storage of information
Expression of information
Variation by mutation
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Figure 10.1
10.2 Until 1944, Observations Favored
Protein as the Genetic Material
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10.3 Evidence Favoring DNA as the
Genetic Material Was First Obtained
during the Study of Bacteria and
Bacteriophages
10.3.1 Transformation: Early Studies
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Figure 10.2
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Table 10.1
10.3 Evidence Favoring DNA as the
Genetic Material Was First Obtained
during the Study of Bacteria and
Bacteriophages
10.3.2 Transformation: The Avery, MacLeod, and
McCarty Experiment
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Figure 10.3
10.3 Evidence Favoring DNA as the
Genetic Material Was First Obtained
during the Study of Bacteria and
Bacteriophages
10.3.3 The Hershey–Chase Experiment
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Figure 10.4
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Figure 10.5
10.3 Evidence Favoring DNA as the
Genetic Material Was First Obtained
during the Study of Bacteria and
Bacteriophages
10.3.4 Transfection Experiments
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10.4 Indirect and Direct Evidence Supports
the Concept that DNA Is the Genetic
Material in Eukaryotes
10.4.1 Indirect Evidence: Distribution of DNA
10.4.2 Indirect Evidence: Mutagenesis
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Table 10.2
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Figure 10.6
10.4 Indirect and Direct Evidence Supports
the Concept that DNA Is the Genetic
Material in Eukaryotes
10.4.3 Direct Evidence: Recombinant DNA Studies
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10.5 RNA Serves as the Genetic Material in
Some Viruses
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10.6 Knowledge of Nucleic Acid Chemistry
Is Essential to the Understanding of
DNA Structure
10.6.1 Nucleotides: Building Blocks of Nucleic Acids
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Figure 10.7
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Figure 10.7a
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Figure 10.7b
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Figure 10.8
10.6 Knowledge of Nucleic Acid Chemistry
Is Essential to the Understanding of
DNA Structure
10.6.2 Nucleoside Diphosphates and Triphosphates
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Figure 10.9
10.6 Knowledge of Nucleic Acid Chemistry
Is Essential to the Understanding of
DNA Structure
10.6.3 Polynucleotides
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Figure 10.10
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Figure 10.10a
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Figure 10.10b
10.7 The Structure of DNA Holds the Key
to Understanding Its Function
10.7.1 Base-Composition Studies
10.7.2 X-Ray Diffraction Analysis
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Table 10.3
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Figure 10.11
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10.7 The Structure of DNA Holds the Key
to Understanding Its Function
10.7.3 The Watson–Crick Model
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Figure 10.12
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Figure 10.12a
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Figure 10.12b
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Figure 10.12c
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Figure 10.13
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Figure 10.14
10.8 Alternative Forms of DNA Exist
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Figure 10.15
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Figure 10.15a
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Figure 10.15b
10.9 The Structure of RNA Is Chemically
Similar to DNA, but Single Stranded
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Table 10.4
10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.1 Absorption of Ultraviolet Light (UV)
10.10.2 Sedimentation Behavior
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Figure 10.16
10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.3 Denaturation and Renaturation of Nucleic
Acids
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Figure 10.17
10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.4 Molecular Hybridization
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Figure 10.18
10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.5 Fluorescent in situ Hybridization (FISH)
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Figure 10.19
10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.6 Reassociation Kinetics and Repetitive DNA
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C/C0 = 1/(1+kC0t)
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10.10 Many Analytical Techniques Have
Been Useful during the Investigation
of DNA and RNA
10.10.7 Electrophoresis of Nucleic Acids
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Figure 10.20