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Investigating the Heart – “a heartfelt dissection” Name Period BACKGROUND INFORMATION The heart is a fist-sized muscle located to the left of the center of the chest. The heart contains four chambers. The upper chambers are called atria. The lower chambers are called ventricles. Between each chamber, there are valves that prevent the backflow of blood. Blood is carried away from the heart by blood vessels called arteries and carried back toward the heart by blood vessels called veins. Arteries and veins are connected by capillaries. Arteries have muscular, elastic walls to help move the blood through the body. Veins have one-way valves to prevent the backflow of blood on its return to the heart. Oxygen-poor blood from cells of the body enters the heart through the right atrium and is pumped into the right ventricle. The blood then travels into the pulmonary artery, which goes into the lungs. In the lungs, the blood gives off carbon dioxide and picks up oxygen. The oxygen-rich blood returns to the heart by way of the pulmonary vein. The blood enters the left atrium and is pumped into the left ventricle. The blood is pumped out of the heart through the aorta to cells in the rest of the body. The muscular wall of the left ventricle is thicker than the wall of the right ventricle because it has to pump the blood to the entire body. Blood is leaving the right ventricle only to go to the lungs. Each time the ventricles contract, blood is forced through the arteries. This force causes a beat, or pulse, that is felt in arteries at the wrist, neck, and temple. The pulse is exactly the same as the heartbeat. In this investigation you will examine the chambers, valves, and blood vessels of the heart. You will also trace the path of blood through the heart. PROBLEM: What are the chambers, valves, and blood vessels of the heart? What path does blood take through the heart? MATERIALS (per group): sheep heart dissecting tray probe metric ruler scissors tweezers PROCEDURE (Day 1) – you should not cut the heart open today!!! 1. Obtain a sheep heart from your teacher. 2. Place the heart in a dissecting tray lined with paper towels. This will help with clean up. 3. A thin membrane called the pericardium still covers the heart. Use a tweezers to remove a small section of it covering the muscle. 4. Describe the muscle that lies underneath the pericardium. For example, does this muscle have polka dots, checkers, smiley faces or what? 5. Find the apex of the heart. Is this at the top or bottom point of the heart? Only the left ventricle extends or goes all the way to the apex. 6. At the opposite end of the apex is the wide end of the heart. Find the opening of the large arteries and veins and the two earlike flaps called auricles. 7. Measure the length of one of the auricles in cm? 8. Measure the width of the heart from auricle to auricle in cm. 9. Measure the length of the heart from top to bottom in cm. 10. Place the heart in your pan with the apex toward you and the smooth round side facing the ceiling. 11. There will be a groove with a blood vessel in it. This is called the coronary artery. As you are looking at the heart, this blood vessel runs diagonally from the right side of the wide end of the heart to a point above and to the left of the apex. 12. Measure the length of the coronary artery in cm. 13. The pulmonary artery should be towards to the top at the wide end of the heart. Point out the pulmonary artery to your teacher or another adult in the room to make sure you are correct. 14. Measure the diameter of the pulmonary artery in cm. 15. The right and left sides of the heart are identified according to the side of the animal’s body in which each is located. As a result, as you look at it, the heart’s right side will be found on your left, and the heart’s left side will be found on your right. 16. The right ventricle now lies to your left and toward the wider end of the heart from the coronary artery. The pulmonary artery to the lungs can be seen curving out of the right ventricle toward the left side of the heart (toward your right). Keep this in mind while dissecting. 17. Locate the two large blood vessels that enter the right atrium. These are the superior and inferior vena cavas. You will need to pick up the heart and look at the back side of it to find the vena cavas. 18. Stick your finger into the superior vena cava (or top one) and have it come out of the inferior vena cava (or bottom one). Both vena cavas enter the right atrium. 19. Go back to your pulmonary artery. Look at the back side of the heart and see the pulmonary artery branches into two holes. You may not see this because of the fat on the heart. These blood vessels (the pulmonary arteries) leave the right ventricle and lead to the . 20. Below the pulmonary arteries are two larger holes. They may be covered in fat where they would be hard to see. These are the pulmonary veins. What part of the heart do the pulmonary veins go into? 21. What part of your body is blood coming from to enter the pulmonary veins to go back into your heart? 22. Find the aorta. When you’re looking at back of the heart, it is the largest hole just above the pulmonary artery. 23. Measure the diameter of the hole of the aorta in cm. 24. Stick your pinky finger into the aorta and see how far down it goes. Be careful not to get your finger stuck. What chamber does blood come from to enter the aorta? 25. If you have extra time, you may cut some fat off of the top of the heart to separate the blood vessels. Just be careful not to cut any of the blood vessels. STOP FOR TODAY!!!! You should not have cut open the heart at all!!! PROCEDURE (Day 2) – you will be cutting the heart open today! 1. Review the outer part of the heart and make sure you know where these structures are: left and right ventricle, left and right atrium, pulmonary artery, pulmonary veins, aorta, coronary artery, apex, superior and inferior vena cava. 2. Locate the pulmonary artery. Put your scissors inside of it and cut through the front side of this blood vessel and continue cutting down through the muscular wall of the right ventricle. This diagonal cutting line should be above and parallel to the coronary artery. Remember the coronary artery is embedded between the right and left ventricles. Stop cutting when you reach the end of the cavity of the right ventricle. 3. You have now cut through the right ventricle. Notice at the beginning of the pulmonary artery you will find a valve. This valve is called the pulmonary valve. Notice that the valve is arranged so that blood can pass from the ventricle out into the pulmonary artery but not in the reverse direction. 4. Look inside the heart. In the upper left of the right ventricle, notice a flap of tissue made of 3 leaflets. This tissue is connected by a bunch of tendons. This is the tricuspid valve. The tricuspid valve sends blood from the right atrium to the right ventricle. Can blood go backwards from the right ventricle to the right atrium? 5. Find the superior vena cava on the back side of the heart again. Cut from the superior vena cava straight down about 3 cm. You will be cutting into the right atrium. Be careful not to cut into the right ventricle. 6. Peek into the right atrium and notice the tricuspid valve (from the other side). Stick your finger into the superior vena cava and through the tricuspid valve. Look through the opening in the right ventricle that you made your first cut into. 7. Find the aorta again. Cut through the aorta until your reach the aortic valve. This valve transports blood from the left ventricle into the . 8. With your scissors you will be making a big cut here!!! 9. Cut through the aorta and continue to cut down through the thick muscular wall of the left ventricle. Compare the thickness of the walls of the left and right ventricle. Which one has thicker walls? 10. Measure the thickness of the wall of the right ventricle in cm. 11. Measure the thickness of the wall of the left ventricle in cm. 12. At the base of the aorta, look at the aortic valve again. 13. Find the mitral valve between the left atrium and the left ventricle. Pass a finger or a probe through it from the left ventricle. Your fingers will be in the left atrium at this point. Try to find the openings of the pulmonary veins which open into the left atrium. 14. Quiz your partner on the interior and exterior anatomy of the heart. You will be tested on this information in a couple of days from both real hearts and models of hearts. 15. Once you are confident that you know the structures, clean up your lab station. Make sure your dissection tools are washed thoroughly and dried completely. The tools will rust if water is inside the dissection kit. Make sure the tray, kit, counter, sink, and floor are free of heart tissue and fat. OBSERVATIONS: In the picture below, write where the blood should be blue and where it should be red. Fill this in using the blanks to the right. All you have to write is red or blue. 1 2 1. 3 2. 3 3. 4 4 5 2 4. 5. 9 6. 6 10 7. 8. 9. 10. 8 7 1. How many flaps of tissue make up the valves between each of the following: a. Right atrium and right ventricle (tricuspid valve) b. Left atrium and left ventricle (mitral valve also called the bicuspid valve) ANALYSIS AND CONCLUSIONS 1. What chambers of the heart receive blood from the body? 2. What chambers of the heart pump blood out of the heart? 3. Which heart chamber has the thickest muscle wall? 4. Why is it necessary for the chamber in the above question to have a thicker muscle wall? 5. How many openings are in the right atrium? 6. What are the openings in the right atrium leading to or from? 7. Which blood vessel brings oxygen poor blood to the heart from the rest of the body? 8. Which blood vessel takes blood from the heart and sends it to the lungs? 9. Which blood vessel takes oxygen rich blood from the lungs and brings it back to the heart? 10. Which blood vessel sends oxygen rich blood from the heart to the rest of the body? 11. How do the valves control the flow of blood through the heart? 12. What is the pericardium? CRITICAL THINKING AND APPLICATION 1. Do all arteries carry oxygen-rich blood? Explain. 2. Sometimes babies are born with a hole in the septum, or wall that separates the right and left sides of the heart. Explain how this situation will affect the baby. 3. On the back page of this lab, label the right atrium and left atrium, right ventricle, left ventricle, superior vena cava, inferior vena cava, pulmonary arteries, pulmonary veins, aorta, pulmonary valve, aortic valve, right auricle, left auricle, septum, coronary artery, and apex. You will be labeling the inside and the outside of the heart so some of the structures will be labeled twice. Use the top and middle heart to label 1-15. The numbers are pointing to the same structures but #s 13, 14, and 15 are not on the top heart. You can use the words from the previous page. 1. 2. 3. 4. 5. 11 skip this one 6. 7. 8. 12 9. 10. 11. 6 1 12. 7 13. 14. 2 13 3 15. 8 9 4 12 15 11 10 Use the heart on the bottom to answer 1-11. 1. 14 2. 3. 4. 5. 6. 7. 11 8. skip this one 9. skip this one 10. 11.