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Eva-Maria Zetsche Personal Details Nationality: University Address: German Department of Marine Sciences University of Gothenburg Visiting Address: Carl Skottsbergs gata 22B, 41319 Göteborg Postal Address: Box 461, 40530 Göteborg, Sweden Tel: Email: Website: +46 (0)31 786 3773 eva-maria.zetsche@gu.se http://marine.gu.se/om-institutionen/personal?userId=xzeeva http://www.nioz.nl/staff-detail?id=10164 Current Research Interests My main research interest now lies in applying a specialized form of microscopy, namely digital holographic microscopy, to the field of marine ecology and in doing so finding novel applications for the technique. I therefore work on a wide range of organisms and particles combining prior knowledge gained throughout my research career with this unique tool. This includes work on algal cells and cyanobacteria but also the polymeric substances they and other organisms can extrude, such as extracellular polymeric substances, transparent exopolymeric particles and coral mucus. In lieu of this work, I sustain my interests in better understanding benthic communities and their ecosystem function, addressing benthic-pelagic interactions such as the organic matter cycling of coral reefs and marine snow aggregate fluxes. Education – Degrees 10/06 – 01/10 Degree of Doctor of Philosophy in Ecology Oceanlab, University of Aberdeen, Scotland. PhD-Thesis: Metabolic Activity in Intertidal Sands: the Role of Permeability and Carbon Sources. Supervisors: Prof. Ursula Witte and Dr. David Lumsdon 10/02 – 09/04 M.Sc. International Studies in Aquatic Tropical Ecology University of Bremen, Germany. M.Sc.-Thesis: Macroalgal Production and Herbivory on Different Reef Substrates of Turneffe Islands Atoll, Belize. Supervisors: Prof. Claudio Richter, Dr. Melanie McField, Prof. GunterOtto Kirst 09/98 – 06/01 B.Sc. (Hons) Oceanography with Marine Biology University of Southampton, England B.Sc.-Thesis: An Investigation into the Bio-Optical Modelling Capabilities of Above-Water Radiometry. Supervisor: Dr. Simon Boxall. Professional Experience 11/15-present University of Gothenburg; Sweden Marie Curie Fellow 08/14-10/15 Royal Netherlands Institute for Sea Research; The Netherlands Postdoctoral researcher 02/10-04/14 Vrije Universiteit Brussel; Belgium Postdoctoral researcher 1 Peer-reviewed Publications Zetsche E, Baussant T, Meysman FJR and van Oevelen D (2016). Direct visualization of mucus production by the cold-water coral Lophelia pertusa with digital holographic microscopy. PLoS ONE 11(2): e0146766. doi:10.1371/journal.pone.0146766 Zetsche E, El Mallahi A and Meysman FJR (in press). Digital holographic microscopy: a novel tool to study the morphology, physiology and ecology of diatoms. Diatom Research. Zetsche E and Ploug H (2015). Marine Chemistry special issue: Particles in aquatic environments: from invisible exopolymers to sinking aggregates. Marine Chemistry, http://dx.doi.org/10.1016/j.marchem.2015.04.008 Zetsche E, El Mallahi A, Dubois F, Yourassowsky C, Kromkamp JC, and Meysman FJR (2014). Imaging-inFlow: Digital holographic microscopy as a novel tool to detect and classify nanoplanktonic organisms. Limnology and Oceanography:Methods, 12: 757-775. doi: 10.4319/lom.2014.12.757 Malkin S, Rao A, Seitaj D, Vasquez-Cardenas D, Zetsche E, Hidalgo-Martinez S, Boschker H, and Meysman FJR (2014). Natural occurrence of microbial sulphur oxidation by long-range electron transport in the seafloor. The ISME Journal, 8: 1843–1854. doi:10.1038/ismej.2014.41 Zetsche E, Meysman FJR (2012). Dead or alive? Viability assessment of micro- and meroplankton. Journal of Plankton Research, 34: 493-509. doi: 10.1093/plankt/fbs018 Zetsche E, Bulling MT, Witte, U (2012). Permeability of intertidal sandflats: Impact of temporal variability on sediment metabolism. Continental Shelf Research, 42: 41–50. doi: 10.1016/j.csr.2012.04.020 Zetsche E, Paterson DM, Lumsdon DG, Witte U (2011). Temporal variation in the sediment permeability of an intertidal sandflat. Marine Ecology Progress Series, 441:49-63. doi: 10.3354/meps09390 Zetsche E, Thornton B, Midwood AJ, Witte U (2011). Utilisation of different carbon sources in a shallow estuary identified through stable isotope techniques. Continental Shelf Research, 31: 832-840. doi: 10.1016/j.csr.2011.02.006. Non peer-reviewed Publications Henneghien J and Zetsche E (2013). “Blending flow analysis with microscopy – the ‘oLine system’ for ballast water monitoring”. The Ballast Times - Volume 4. Zetsche E and Henneghien J (2013). APPLICATION NOTE - OVIZIO Imaging Systems D3HM: Counting small single-celled aquatic organisms with Ovizio’s D3HM in static mode. Gollash S, Zetsche E and Henneghien J (2012). Demonstration of the OVIZIO Organism Detection Technology, the 'oLine', for Compliance Enforcement with the Biological Standards of the IMO Ballast Water Management Convention. Final report. Prepared for Interreg IVB North Sea Ballast Water Opportunity project: 14 pp. Manuscripts in progress Zetsche E, Larsson A and H Ploug. Characterizing flow and diffusion around and within marine snow aggregates as a function of porosity and TEP content (in prep). Zetsche E, Engel A and FJR Meysman. Abundance and distribution of non-living organic matter in coral reefs (in prep). 2 Grants, scholarships and awards 2015 2014 2014 2014 2013 2012 2012 2011 2011 2009 2008 2008 2006 2006 2003 Awarded an Individual Fellowship of the Marie Sk!odowska -Curie Action (H2020-MSCAIF-2014). Project title: Bio-physical processes around marine snow aggregates. ASSEMBLE grant (9th call) for on-site access to the Sven Loven Center for Marine Research in Tjärnö, Sweden. Project title: „SNOW-FLOW: Characterizing flow and diffusion around and within marine snow aggregates as a function of porosity and TEP content.” ASLO Early Career Travel Grant for attendance of the Ocean Sciences Meeting 2014. FWO ‘Grant for participation in an international conference’ for the attendance of the Ocean Sciences Meeting 2014. ASSEMBLE grant (7th call) for on-site access to the Interuniversity Institute for Marine Sciences (IUI) in Eilat. Project title: „CORAL-DYNLOM: Dynamics of non-living organic matter in coral reefs: Combining classical staining and microscopy techniques with novel digital holographic microscopy.” HoloFlow II: Advanced digital holographic microscopy and 4D imagery for applications in water technology and environmental monitoring. INNOVIRIS Impulse Programme Environment 2008. Renewal proposal written amongst project partners. ASLO Early Career Travel Grant for attendance of the ASLO Aquatic Sciences Meeting. ASLO Early Career Travel Grant for attendance of the ASLO Aquatic Sciences Meeting. Travel support for attendance of the Microscale Interactions Symposium 2011. ASLO PhD Student Travel Grant for attendance of the ASLO Aquatic Sciences Meeting. Best student oral presentation award at the 10th International Estuarine Biogeochemistry Symposium (2008). “Temporal variation in sediment permeability changes the metabolic functioning of estuarine sands.” Travel grant for attendance of 13th Biennial Challenger Conference for Marine Science. ACES PhD Studentship (Aberdeen Centre for Environmental Sustainability), 2006-2009 Scholarship awarded for attendance of the Coral Reef Ecology Course at BIOS (Bermuda) from the UK Associates and CV Starr and Ernest E. Stempel Foundations. Funding for MSc fieldwork in Belize by a DAAD-Scholarship (German Academic Exchange Service) Conferences / Workshops 22 – 27/02/15 ASLO Aquatic Sciences Meeting 2015. Oral presentation. “Imaging-in-flow: digital holographic microscopy as a novel tool to detect and classify nanoplanktonic organisms”. 23 – 28/02/14 Ocean Sciences Meeting 2014. Honolulu, Hawaii. Poster presentation and session co-chair. “Abundance and distribution of non-living organic matter in coral reefs”. 06 – 11/10/13 Batsheva de Rothschild Seminar on Marine life in the flow: Workshop on physical-biological interactions from the individual organism to the global scale. InterUniversity Institute for Marine Sciences (IUI), Eilat, Israel. Poster presentation and hands-on demonstration sessions of Ovizio’s fluorescence add-on module (qMod): “Digital holographic microscopy – a unique tool for studying physicalbiological interactions” 10 – 15/03/13 Microscale Interactions Symposium 2013 - Microscale Interactions in Aquatic Environments. Les Houches, France. Oral presentation. “Digital holographic microscopy: Visualizing the ‚invisible’.” 11 – 14/02/13 NIOZ Flow Cytometer Workshop: Comparing instruments in measuring 2-10 !m and 10-50 !m plankton cells. Texel, Netherlands. Active Participation with an oLine D3HM instrument. Oral Presentation with Joël Henneghien (Ovizio): “New 4D quantitative imaging platform.” 26 – 31/08/12 22nd International Diatom Symposium. Gent, Belgium. Poster presentation. “Digital holographic microscopy as a useful tool for analysing algae. 08 – 13/06/12 ASLO Aquatic Sciences Meeting: Voyages of Discovery. Otsu, Japan. Oral presentation and session co-chair. “Visualizing the “invisible” – using digital holographic microscopy to quantify biologically produced exopolymers”. 3 24 – 25/05/12 North Sea Ballast Water Annual Meeting 2012. Hamburg, Germany. Oral presentation. “Ovizio Organism Detection Technology for Compliance Enforcement with the Biological Standards of the IMO Ballast Water Management Convention.” 13 – 18/02/11 ASLO Aquatic Sciences Meeting: Limnology & Oceanography in a changing world. San Juan, Puerto Rico. Oral presentation. “Alive or dead? Combining staining methods and digital holographic microscopy for viability determination of micro- and mesoplankton”. 01/02/11 2nd Belgian Coral Meeting. Mons, Belgium Poster presentation. “Future use of digital holographic microscopy in coral reef science.“ 17 – 21/01/11 Aspen Ocean Symposium: Microenvironments modulating biological interactions in the ocean. Colorado, USA. Poster presentation. “Improved digital holographic microscopy”. 13 – 17/12/10 Euro ISRS Symposium 2010: Reefs in a changing world. Wageningen, Netherlands. Poster presentation. “Future use of digital holographic microscopy in coral reef science.“ 18 – 19/08/09 EOS Topical Meeting on "Blue" Photonics - Optics in the Sea. Aberdeen, UK. 23/02/09 1st ACES Symposium. The Macaulay Institute, Scotland, U.K. Poster presentation. 25 – 30/01/09 ASLO Aquatic Sciences Meeting: A Cruise Through Nice Waters. Nice, France. Oral presentation. 08 – 11/09/08 13th Biennial Challenger Conference for Marine Science. “Our Changing Oceans”. Bangor, Wales, U.K. Poster presentation. 18 – 22/05/08 10th International Estuarine Biogeochemistry Symposium – Estuaries in a changing world. State Key Laboratory of Marine Environmental Science, Xiamen, China. Oral presentation. 19 – 22/09/06 International Society for Reef Studies - European Meeting. University of Bremen, Germany. Poster presentation. Invited talks and seminars 13/12/13 GEOMAR - Helmholtz Centre for Ocean Research Kiel. Germany. “Digital holographic microscopy in the marine sciences” 04 – 05/06/13 Royal Netherlands Institute for Sea Research (NIOZ) Science Days. Noordwijkerhout, The Netherlands. Invited Talk: “The possibilities of the DHM for oceanographic applications”. 13/05/13 Weizmann Institute of Science. Rehovot, Israel. “Digital holographic microscopy: a unique tool in the study of phytoplankton” 24 – 27/06/13 Marine Biology Live Cell BioImaging Conference. Roscoff, France. Invited Talk. “Digital holographic microscopy: Visualizing the “invisible” in marine science applications”. 15/06/12 Marine Station of Wimereux (Université des Sciences et Technologies de Lille). France. “Advanced digital holographic microscopy and 4D imagery for oceanographic applications” 01/12/11 Max-Planck-Institute for Marine Microbiology. Bremen, Germany. “Advanced digital holographic microscopy and 4D imagery for oceanographic applications” Teaching/Responsibilities 02/16 Practical teaching for MSc Course Primary Producers of the Sea (BIO458) University of Gothenburg); Laboratory exercise in microalgae-bacteria interactions: “The biological carbon pump”. 4 09/13 – 12/13 Co-supervision of MSc Internship (NIOZ-Yerseke). Project title: Monitoring of aquatic microorganisms using differential digital holographic microscopy. 10/13 – 10/14 Co-supervision of MSc Internship (NIOZ-Yerseke). Project title: Electricity!generating bacteria in the seafloor: optical characterization of extracellular polymeric sheaths. 02/08 – 05/09 Demonstrator (University of Aberdeen) Assistant for practicals for 2nd year undergraduate course “Ocean Biology” 02/07 – 02/09 Tutorial assistant (University of Aberdeen) Supervision support for several projects: • • • Undergraduate (4th year) Honours project on carbon sources in the Ythan Estuary. Leonardo placement scholarship (university student) and Nuffield Science Bursary (high school student) investigating seasonal variation of macrofaunal abundances for an intertidal sand sediment. Undergraduate project in small-scale spatial variability of sediment permeability Courses / Workshops 19 – 23/05/14 Diffusion in aquatic environments. University of Gothenburg, Sweden. Course leader: Prof. Helle Ploug (UGOT). 29 – 30/10/13 Scientific Image Processing and Analysis. NIOZ-Yerseke, The Netherlands. Course run by Dr. Jan Brocher (BioVoxxel, Germany), organised by E. Zetsche. 11 – 14/02/13 NIOZ Flow Cytometer Workshop: Comparing instruments in measuring 2-10 !m and 10-50 !m plankton cells. Texel, Netherlands. 28 – 31/01/13 In situ detection of microbial structure and function in the environment. NIOZYerseke, The Netherlands. Training course run by Prof. N. Lee (Technical University Munich, Germany). 25 – 26/01/12 Chemotaxis assays and video microscopy. Munich, Germany. Training course run by Ibidi GmbH. 16 – 17/11/11 Cell cultivation under perfusion and live cell imaging. Munich, Germany. Training course run by Ibidi GmbH 03 – 22/08/08 Aquatic Microbial and Molecular Ecology Training Course. University of Southern Denmark, Odense, Denmark. Supported by a travel award. 27/07/07 Eco-Imagine GIS and ICZM Geospatial Technologies Training Course. Aberdeen Exhibition and Conference Centre, Aberdeen, U.K. 18 – 22/06/07 MarBEF Training Course on Measuring Biodiversity and Ecosystem Function in Estuarine Systems. Gatty Marine Laboratory, St Andrews University, U.K. 09 - 29/07/06 BBRS Coral Reef Ecology Training Course. Bermuda Biological Station for Research (now BIOS), Bermuda. Supported by a scholarship. Reviewing and Editorial Services Guest editor for Marine Chemistry special issue: “Particles in aquatic environments: from invisible exopolymers to sinking aggregates” (2015). Reviewer for Biogeosciences, Geochimica et Cosmochimica Acta; PLOS One; Hydrobiologia; International Journal of Water Resources and Environmental Engineering. General Skills 5 Languages: German and English – fluent, Dutch – moderate, French – basic. IT: Microsoft Office, R-Package, SigmaPlot, LaTeX, ArcView (GIS), ImageJ Other: PADI Diver Master Certification Interests Travel; photography particularly underwater photography; swimming Gothenburg, 10.03.2016 6