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Application recommendations COMPO Sun Protect contains active ingredients which have to be taken up by the plant surface and will be active after having reached the target tissue. In most cases this process requires up to 24 hours from the application time on. Thus it is strongly recommended to spray COMPO Sun Protect at least 24 hours before the expected high-radiation period. The higher level of anti-oxidative agents in the crop tissue will last for about 7 to 10 days. After this period it will be necessary to repeat the application. Spraying should be done when there is a high probability for a period of dry days with high solar irradiation during a sensitive stage of the crop. This will never be completely predictable but own observations or the local weather forecast will reduce the risk of sunburn damages to a minimum. COMPO Sun Protect – How does it work? COMPO Sun Protect has been created to prevent fruits and other susceptible plant parts from sunburn. It combines UV absorbing compounds with the most effective natural antistress compound α-tocopherol. While α-tocopherol eliminates ROS and stabilizes the cell membranes, special UV absorbers prevent the radiation-induced destruction of cell structures. COMPO Sun Protect Boron is also added to COMPO Sun Protect because this micronutrient has proven to be very effective in enhancing the stress tolerance of plants by stabilizing the cell wall and its function. Furthermore boron plays a major role in regulating the permeability of cell membranes. It is recommended to apply COMPO Sun Protect in a quantity of 1.2 liter solution per hectare. To ensure the ideal concentration of the active ingredients 1.2 liter of COMPO Sun Protect should be dissolved in 200 liters of water (= 0.6%). For single plant treatments prepare also 0.6% solutions and spray until the whole plant is wet. Split applications at lower concentrations, for example two times 0.6 liters per hectare at an interval of 5 to 7 days will extend the effective time period. COMPO Sun Protect prevents valuable crops from sunburn due to its physiologically active ingredients saves the grower from significant yield losses EXPERTS FOR GROWTH COMPO Sun Protect for Fruit crops and grapevines: Less sunburn and more marketable fruits Vegetables and ornamental crops: Better growth of young plants after transplanting due higher stress tolerance COMPO Sun Protect Fig. 7: Synergistic action of the COMPO Sun Protect ingredients in sunburn prevention Prevents valuable fruits from sunburn due to its physiologically active ingredients Components: COMPO Sun Protect is a borated fertilizer. Specific weight: 1.07 kg/l pH: 7.3 Over-heating of tissues α-tocopherol Boron www.compo-expert.com Saves the grower from quality yield losses Stabilization of cell membranes Stabilization of the pectic network between the cell walls Regulation of membrane permeability Cell damage induced by UV radiation Phenolics acids and UV absorbers 5 Elimination of ROS and radicals Protection from radiation Folder Sun 2012 α-DL-tocopherol Phenolic acids UV absorbers Boron (2%) Specific adjuvants to ensure uptake and enhanced efficiency Anti-oxidizing effects Germany COMPO GmbH & Co. KG Gildenstraße 38 48157 Muenster Phone: +49 (0) 251 3277-0 Fax: +49 (0) 251 326 225 e-mail: info@compo.de www.compo-expert.com on the fruit variety and the local climate. In growing regions with hot and dry summers, especially at higher altitudes, the phenomenon of fruit sunburn is wellknown. Modern varieties like “Pink Lady” apples or “MD2” pineapples are very susceptible to high solar irradiation and rapidly loose their outstanding quality. Fig. 1: Sunburn damage in pumpkin, grape and orange fruit Both stress factors, heat and UV radiation, result in the formation of reactive oxygen species (ROS) and chemical radicals. These species destroy cell membranes through oxidation (“oxidative stress”) and finally lead to cell death. Plants respond to oxidative stress with the formation of antioxidants, which eliminate ROS and radicals and prevent the cell membranes from disintegration (figure 3). The most prominent antioxidants in plants are α-tocopherol (“Vitamin E”), ascorbic acid (“Vitamin C”), carotenoids and phenolic compounds. Among these compounds, α-tocopherol is the most effective natural antioxidant: 1 molecule of α-tocopherol inactivates up to 220 radical molecules! Fig. 3: Effect of oxidative stress and plant response Oxidative stress (Frost, Heat, Radiation, …) Plant response: Formation of ROS . (H2O2; O2 ) Formation of natural antioxidants Damage of cell membrane Neutralization of free radicals Fig. 2: Severe apple fruit damage due to sunburn, South Africa 2012 Loss of cell functionality Preservation of cell functionality Death of the cell No damage The major function of α-tocopherol is to keep the cell membranes functioning even under severe environmental stress like over-heating. In this process α-tocopherol is working Fig. 5: Significant increase of α-tocopherol concentration in “Elstar” fruit peels 24h after application of COMPO Sun Protect hand-in-hand with ascorbic acid. Whereas ascorbic acid is mostly available in sufficient concentrations in the plant cell, α-tocopherol is the limiting factor in the antioxidative defence reaction. 0,8 Control 35 COMPO Sun Protect 30 When does sunburn damage occur? Sunburn damage in fruits is supposed to be a consequence of a) local overheating over the fruit skin and tissue and b) caused by temporarily high UV radiation. 25 20 15 10 5 0 On warm sunny days the fruit skin temperature may be 10 to 15°C above the ambient air temperature. This means 35°C measured in the air temperature can easily result in an apple skin temperature of 50°C or higher, which directly leads to local overheating and subsequently to skin lesions. Beside air temperature UV radiation is able to damage plant tissues. With every 1000 m increase in elevation there is a plus of 10 to 12% more UV-A radiation on the ground. The share of UV also increases with lower latitudes. Thus the most endangered zones are mountain regions at lower latitudes. UV radiation will be readily absorbed by the air humidity, therefore there is a higher risk for UV damage during dry weather periods. Injuries caused by tissue over-heating typically result in brown or black sharp-edged spots on the fruit surface. This happens easily when the fruit skin temperature exceeds 52°C for about 10 minutes. At lower temperatures (48°C) and in combination with UV radiation the corresponding skin disorder will appear more like a diffuse brownish area. Fig. 6: Recovery of chlorophyll fluorescence in Golden Delicious apple fruits after COMPO Sun Protect application and exposition to UV light (1.8 kJ/m2 for 14h) 0,5 0,4 0,3 0,2 0,1 Control - UV Tab. 1: Sunburn damage in Golden Delicious fruits after UV light exposition and treatment with COMPO Sun Protect (1.8 kJ/m2 for 14h) Control (no UV light) 0,6 0 24 h Hours after application Treatment 0,7 Chlorophyll-Fluorescence (Fv/Fm) Sunlight is the driving force for growth and development of green plants. In the process of photosynthesis solar energy is transformed into carbohydrates, the basis for human nutrition. However, in some cases sunlight induces severe damage of plant tissues, especially in fruits. Nearly all kind of high-value fruits are susceptible to light-induced skin damage: apples, pears, peaches, citrus, pineapple and many more. Sunburncauses fruit yield losses in the range of 10 to 50%, dependent What happens in the plant tissue when it is exposed to excessive high temperature and UV radiation? α-tocopherol (µg/g FM) Sunburn damage in crop production Sunburn rating* (0-3) 0 Apples exposed to UV light 2.23 COMPO Sun Protect 1.26 COMPO Sun Protect Control + UV Tab. 2: Influence of COMPO Sun Protect treatments in the field on sunburn incidence in „Granny Smith“ apples (South Africa) Fruits with sunburn spots (%) Treatment none low medium strong Control 56.5 13.8 21.1 8.6 α-tocopherol (0.1%) + ethylhexyl methoxycinnamate 96.3 3.7 0.0 0.0 * 0 = no, 1 = light, 2 = medium and 3 = severe damage Fig. 4: Air Temperature (blue) and corresponding fruit surface temperature (red) in a pome granate orchard Fruit Temp. Air Temp. Temperature (°C) 50 45 40 This graph illustrates the relationship between air temperature & fruit surface temperature. Note that fruit surface temperature can be considerably higher than air temperature, and it is fruit surface temperature that determines the level of sunburn. 35 30 25 2 www.compo-expert.com 8 9 10 11 12 Time 13 14 15 16 Source: Yazici and Kaynak, 2006 www.compo-expert.com 3 www.compo-expert.com 4