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THE CELL Unit six Entry task Look through your notes and find the 6 steps of cell division. Animal and Plant Cell Questions 1. 2. 3. 4. 5. What are the similarities and differences between plant and animal cells (create a list of an answer of at least 3-4 sentences)? Why don’t animal cells need cell walls? What does the cell wall do for the plant cell? What does the chloroplast do? What are the similarities and differences between cellular respiration and photosynthesis (minimum of 3-4 sentences)? Entry Task What’s the difference between these two cells? Objectives Explain hybridization Describe self-pollination and cross pollination and explain how they are different Understand the basic structure of DNA and how they encode for protein molecules through RNA Understand Meiosis Understand Mutations Know the three regions of growth on plants Name three functions of cells Understand the relationship between DNA, genes, and chromosomes Explain how growth regulators are used on plants Describe the sequencing of bases in DNA Discuss environment versus heredity on plant growth Vocabulary Abscisic Acid Adenine Alleles Bases Chromosome Codons Cytosine Differentiation Ethylene Gametes Genes *Genome * Guanine * Hormone * Metabolites *Mitosis *Morphogenesis *Nucleotides *RNA *Senescence *Thymine Regions of Growth Growth usually takes place in two ways. Enlargement of a single seed, leaf, stem, root, or fruit Formation of new cells in a specialized part of the plant. Due to intake of water or other substances into previously formed cells If this region of the plant is destroyed, new growth will not occur. Three areas of new growth Tips of stems (terminal bud) and roots Axils of leaves Increase length Formation of new stems, leaves, and flowers Cambium layer in stems and roots Puts growth (width) on tree trunks and branches Concepts and components of growth Plants have the ability to create new growth in regions called meristems. Plants also have the ability to change their cells. This process of cell change is called differentiation. Cells All living material is made of cells or the chemical products of cells. Cellular biology states: All living material is made up of cells or the product of cells All cells are derived from previously existing cells; most cells are created by cell division…sex organs are created differently A cell is the most elementary unit of life. Every cell is bounded by a plasma membrane. All cells have strong biochemical similarities. Most cells are small, about 0.001 cm What's a cell suppose to do? Maintenance Synthesis of cell products Cell division These functions require the cell to take in nutrients and excrete waste. ATP is the universal energy transfer molecule and is created during photosynthesis! Morphogenesis All organisms begin as a single cell. Through mitosis (division) and repeated cell growth, organisms develop into an adult, containing billions of cells. A plant has many different types of cells. These cells must go through differentiation into a specialized cell type (leaf, stem, xylem, ect.) Chromosome..what makes a cell a cell The genetic code found in cell is what defines the shape that cell will take. The information needed to program and guide the growth is contained within chromosomes. Each cell contains the SAME genetic information that was present in the fertilized egg. But each cell is different because of various biochemical triggers that turn different genes on and off to produce various proteins. Mitosis Mitosis is the reproduction of cells in which the genetic material of the cell is duplicated exactly. The cells simply divide and produce new cells like themselves (daughter cells). Six steps of mitosis 1. 2. 3. 4. 5. 6. Interphase Prophase Metaphase Anaphase Telophase Interphase Genes contain the blueprint or code that determines how the plant will look and interact with its environment. Not all plants have the same number of chromosomes. Genes are the basic unit of inheritance. Genes are carried in the chromosomes in the Gametes…egg or pollen. Genes are made of DNA. Genes cause the production of enzymes, which control chemical reactions in the plant, thus affecting plant development and function. For normal development and function, genes must occur in pairs. Genes are a pair of chromosomes that reside in the nucleus of the cell. Chromosomes in the nucleus of a certain cell contain the same genetic information as the chromosomes in every other cell. The genes in the roots are the same as the genes in the stem. Description of genes The complete set of instructions for making a plant is called its genome. Think of this as the blueprint of a house The genome is found in every nucleus of the cell and consists of tightly coiled threads of DNA and proteins, organized into chromosomes. There are 150 million bases found in DNA If unwound and tied together the strands of DNA would stretch more than 5 feet but would be 50 trillionths of an inch wide. For each organism the components of these slender threads encode all the information necessary to build and maintain life. This is true for all life from bacteria to blue whale. Structure of DNA DNA molecules consist of two strands that wrap around each other to resemble a twisted ladder. The sides are made of sugar and phosphate molecules and connected by rungs of nitrogen containing chemical bases. DNA strands Each strand of DNA is a linear arrangement of repeating similar units called nucleotides, which is each composed of one sugar, one phosphate, and a nitrogenous base. The four different bases of DNA are called: Adenine Thymine Cytosine Guanine These strands are held together by a weak bond between the bases creating a pair. Each time a cell divides into two daughter cells its full genome is duplicated. During cell division each strand of DNA unwinds and weak bonds are broken between base pairs….the strands will separate. Once strands are separate the base begins to pair once again with their complementary base. Base pairing These bases will ALWAYS pair with one another. Adenine will pair only with thymine Cytosine will pair only with guanine. Each daughter cell receives one old and one new DNA strand. Cells following the base-pairing rules ensure that the new strand is an exact copy of the old one. This minimizes mutations How the code works Each DNA molecule contains many genes, the basic physical and functional units of heredity. A gene is a specific sequence of nucleotide bases whose order carry the information required for constructing proteins, which provide the structural components of cells and tissues. Each three base code calls for specific amino acids to bind to. mRNA is used to create duplicate DNA Control of growth What factors do you think control plant growth? Control of growth What factors do you think control plant growth? Heredity – controls species characteristics, sets limits to size and rate of growth through enzyme production and cell enlargement/division. Hormones- growth regulators. Organic chemicals produced in specific area of the plant that causes a physiological response. Cytokinins: cell division Auxins: cell elongation Gibberellins: cell elongation Controls things like stems growing towards light, root growth, fruit and flower development, and leaf enlargement. Nutrition- growth regulator Water: result of enlargement due to internal water pressure (turgor). Calcium: interacts with auxin and cytokinins to regulate cell division and elongation. Nitrogen: involved in the structure of chlorophyll, proteins, auxins, and cytokinins Environment Temperature: all physiological activities are directly related. Warmer = more favorable. Cooler = sometimes needed for initial plant growth. Light: wavelength affects pigmentation in leaves. More on hormones Auxins: primary control of growth through cell enlargement. Sometimes acts as both stimulator and inhibitor of growth. Affect: shoot, buds, and roots. Also stimulate differentiation of cells Gibberellins: control cell elongation and division in plant shoots Cytokinins: cell division, cell enlargement, and transportation of amino acids. Review Questions: T/F The complete set of instructions for making an organism is called its genome. Differences in genetic makeup are often referred to as genetic variation The environment has little effect on the expression of the gene pairs controlling a trait. Review Questions: Short Answer Name the three functions of all cells. List five basic assumptions about all cells Name four general examples of plant hormone What pairs with these bases: adenine, adenine, cytosine, thymine, and adenine? List three parts of plant where new cells continue to be formed. Review questions: Critical thinking Give an example of how heredity and the environment interact to alter plant growth and development. Discuss three ways that plant hormones affect plants or plant cells