Download Powerpoint - Blood Journal

MicroRNA-29a and microRNA-142-3p are regulators of
myeloid differentiation and acute myeloid leukemia
by Xiao-Shuang Wang, Jia-Nan Gong, Jia Yu, Fang Wang, Xin-Hua Zhang, Xiao-Lin
Yin, Zhen-Qing Tan, Zi-Mian Luo, Gui-Hua Yang, Chao Shen, and Jun-Wu Zhang
Blood
Volume 119(21):4992-5004
May 24, 2012
©2012 by American Society of Hematology
Transfection of THP-1 and NB4 cells with miRNA mimics or antisense inhibitors for miR-29a and
miR-142-3p affects myeloid differentiation.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
CCNT2 is a target gene of both miR-29a and miR-142-3p, and CCNT2 plays a role in inhibiting
PMA-induced monocytic differentiation in THP-1 and HL-60 cells.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
Validation of CDK6 and TAB2 as target genes for miR-29a and miR-142-3p, respectively.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
Expression inhibition of CDK6 and TAB2 increases ATRA-induced differentiation in NB4 cells
and PMA-induced differentiation in THP-1 cells.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
Role of miR-29a and miR-142-3p in CD34+ HSPCs derived from UCB. CD34+ cells purified from
human UCB samples were infected with Lenti-control, Lenti-29a, or Lenti-142.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
CDK6, TAB2, and CCNT2 expression levels in monocytic and granulocytic induction cultures of
CD34+ cells.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology
Expression of miR-29a and miR-142-3p in AML BM blasts and their function in promoting
myelopoiesis.
Xiao-Shuang Wang et al. Blood 2012;119:4992-5004
©2012 by American Society of Hematology