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Supplementary Table 1 PCR amplification primers used in this study
Primer
Term
qPCR
F
Term
qPCR
R
Q
qPCR
F
Q
qPCR
R
Cro
qPCR
F
Cro
qPCR
R
Capsid
qPCR
F
Capsid
qPCR
R
cI
qPCR
F
cI
qPCR
R
16s
qPCR
F
16s
qPCR
R
GyrB
qPCR
F
GyrB
qPCR
R
P1
qPCR
F
P1
qPCR
R
P2
qPCR
F
Sequence (5' – 3')
CGGTTTGTTCATTGCCTTCTCAACCG
CCTAAATACAGCGCCAGAGTGC
GTAAAATCACGTCCACAGTGC
AACACGTAATAATCAACCAGC
Dilution
range in
gene
copy
number
R2
Slope
Proposed
function
5.08
x105 2540
0.990
-3.22
Cleaves
concatemeric
phage DNA,
expressed during
the lytic cycle
4.47
x105 1830
0.992
-3.27
Antiterminator,
expressed during
the lytic cycle
-3.07
Transcription
repressor,
expressed during
the lytic cycle
-3.10
Structural gene,
Antiterminator,
expressed during
the lytic cycle
0.998
-3.17
Transcription
repressor
necessary to
establish lysogeny,
expressed in the
lysogen
0.995
-3.66
AAAGGGCTGTCTATAAGTGG
7.61x105
- 1520
0.992
GCCACCAGAAATCTCTTCG
AGGTGCCTGCGAAGCTATTC
GCTCTCCTGGTCACGACG
GTGAGGGAACGGAGCTACAG
GCGGCCTTATGCTTTCAATG
CATCGAGGAACGGTACGAGA
CGATCTCGGTAAAGTCGTCGAT
GTCGAAGTGGCGTTGCAGTG
AGCCTGCCAGGTGAGTACCG
4.47
x105 1830
3.55
x106 1550
6.35
x105 1270
7.12
x105 4060
0.991
0.996
Cell marker
-3.12
Target of
norfloxacin, used
as induction
marker
-3.21
Phage gene of
unknown function
-3.68
Putative RuvC
resolvase
CAGCGTTTGCATAAGCC
3.55x105
- 1730
0.997
CGTGAAAAGGCAGAGAAAGC
CGGATACCATGCGGACG
3.55
x105 1730
0.996
P2
qPCR
R
P3
qPCR
F
P3
qPCR
R
P4
qPCR
F
P4
qPCR
R
P5
qPCR
F
P5
qPCR
R
P6
qPCR
F
P6
qPCR
R
CM1
qPCR
F
CM1
qPCR
R
CM2
qPCR
F
CM2
qPCR
R
CM5
qPCR
F
CM5
qPCR
R
CM7
qPCR
F
CM7
qPCR
R
CM18
qPCR
F
CM18
qPCR
R
CGTTTTGCCGTTCTTTTTGGTGG
GCGGTGTGACTTCAATATTTC
GCTGCCATACGCGTTACTGAATC
GGATTCAGTAACATTCACGCCG
GCAAAACCCCGATCAGGAAAGAAG
5.51
x105 2360
5.51
x105 2360
0.990
0.991
-3.50
Phage gene of
unknown function
-3.25
Putative lambda
like exonuclease
GCAGAGAGCGGTGAAGTTCAGC
CGTCTCCGTCACTTCCTGCAG
4.47
x105 1830
0.998
-3.28
Phage gene of
unknown function
5.51
x105 2360
0.998
-3.36
Phage gene of
unknown function
-3.70
Tail spike protein
GCAAAACGGCAAGAAAAACCACC
GCCTATGGTACGCCTGC
GAAATCTCCTGATGGTGAGG
GATCCATCGTCATTCC
AAGGACTGCTGGCAAACG
GTCGGCCTCAGTTAGC
TTATCACCGTCACAATTTGC
CTGCTTACACTGTAAGAACG
TATGGCCTATTCAGAGG
AACGACGTACTGTTATCC
TGAAAGCAACAGCACG
CCAATGCCAGCAGTAACG
3.55
x105 1730
0.996
5.85
x105 4560
0.990
-3.25
Putative Dam
methylase
7.12
x105 1620
0.997
-3.45
Phage gene of
unknown function
3.55
x105 1730
0.996
-3.37
Phage gene of
unknown function
3.55
x106 1550
0.990
-3.16
Homologous to
Lambda lom gene