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Evolution & Microevolution Tutorial Introduction Microevolution Hardy Weinberg Equilibrium Practice! In this tutorial, you will learn: The difference between macroevolution & microevolution. How Hardy-Weinberg equilibrium works as well as factors that can upset this equilibrium. How to use the equation, p2 + 2pq + q2 = 1, to calculate allele frequencies in a population. Credits: Figures and images by N. Wheat unless otherwise noted. Lesser ball python image used with permission from Tim Bailey, Bailey & Bailey Reptiles. Funded by Title V-STEM grant P031S090007. Introduction Evolution – includes all of the changes in the characteristics and diversity of life that occur throughout time. Evolution can occur on both large and small scales. Macroevolution – Evolution on a Large Scale Macroevolution a grand scale. – evolutionary change on Origin of novel designs Evolutionary trends Adaptive radiation Microevolution – Evolution on a Small Scale Microevolution - a change in the genetic composition of a population over time. A change in the frequency of certain alleles in a population over several generations. Polymorphism Polymorphism occurs when there are different allelic forms of a gene in a population. Mojave (left) and Lesser (middle) are different alleles of the same gene. Wild type ball python is shown on the right. Photo courtesy of Bailey & Bailey Reptiles Gene Pool All of the alleles of all of the genes possessed by all of the members of the population are contained in the gene pool of the population. We can measure the relative frequency of a particular allele in a population. Allelic frequency Population Genetics Population Genetics – the study of how populations change over time. Dependent on both Darwin’s theory of natural selection and Mendel’s laws of inheritance. All heritable traits have a genetic basis, some are controlled by multiple genes – not as simple as in Mendel’s studies. Genetic Equilibrium According to Hardy-Weinberg equilibrium, the hereditary process alone does not produce evolutionary change. Allelic frequency will remain constant generation to generation unless disturbed by mutation, natural selection, migration, nonrandom mating, or genetic drift. These are sources of microevolutionary change. Frequency of Alleles Each allele has a frequency (proportion) in the population. Example population of 500 wildflowers. CRCR = red; CRCW = pink; CWCW = white 250 red, 100 pink, 200 white Frequency of CR = (250 x 2) + 100 / 1000 = 600/1000 =.6 = 60% Frequency of Alleles p is the frequency of the most common allele (CR in this case). q p = 0.6 or 60% is the frequency of the less common allele (CW in this case). p+q=1 q = 1- p = 1 – 0.6 = 0.4 or 40% Hardy-Weinberg Theorem Populations that are not evolving are said to be in Hardy-Weinberg equilibrium. Hardy-Weinberg Theorem As long as Mendel’s laws are at work, the frequency of alleles will remain unchanged. Review Punnett squares in the genetics tutorial. Hardy-Weinberg Theorem The Hardy-Weinberg theorem assumes random mating. Generation after generation allele frequencies are the same. Hardy-Weinberg Theorem Conditions required for Hardy-Weinberg equilibrium to hold true: Very large population No gene flow into or out of the population No mutations Random mating No natural selection Hardy-Weinberg Theorem Departure from these conditions results in a change in allele frequencies in the population. Evolution has occurred! Practice with Hardy Weinberg Frequency – the proportion of individuals in a category in relation to the total number of individuals. 100 cats, 75 black, 25 white – frequency of black = 75/100 = 0.75, white =0.25. Two alleles: p is common, q is less common. p+q =1 Question 1 The frequency of black cats is: 0.75 75 0.25 25 100 Question 1 Sorry! That is incorrect. Try again! Question 1 Congratulations! You are correct! Question 2 What would the frequency of black cats be if the population size was 80 instead of 100 (still 75 black)? 0.75 75 0.94 1 (75/80) Question 2 Sorry! That is incorrect. Try again! Question 2 Congratulations! You are correct! Hardy-Weinberg Theorem At a locus with two alleles, the three genotypes will appear in the following proportions: (p + q) x (p + q) = p2 + 2pq + q2 = 1 Practice with Hardy Weinberg (p + q)2 = p2 + Individuals homozygous for allele B 2pq + Individuals heterozygous for alleles B &b q2 Individuals homozygous for allele b Practice with Hardy Weinberg We will use a population of 100 cats as a practice example. 84 of the 100 cats are black. 16 are white. Practice with Hardy Weinberg We can use the equation and our color observations to calculate allele frequencies in our population of 100 cats. p2 + 2pq + q2 = 1 100 = population size Practice with Hardy Weinberg 84 of our 100 cats are black. Black is the dominant phenotype. Cats with the genotype Bb or BB will be black. The frequency of black cats is 84/100, but we can’t yet say anything about the B allele. See the genetics tutorial to review these terms. Practice with Hardy Weinberg 16 of our 100 cats are white. White is recessive (bb) and is represented by q2 in our equation: p2 + 2pq + q2 = 1 So, q2 = 16/100 = 0.16 q = square root of 0.16 = 0.40. Practice with Hardy Weinberg q = square root of 0.16 = 0.40. Since p + q = 1; p = 1 – q = 0.60. p2 = 0.36 p2 represents the proportion of individuals in the population with the homozygous dominant phenotype (BB). Remember population size = 100 Question 3 So, the number of cats in our population that have the BB genotype would be: 0.36 cats 0.36 x 100 = 36 cats 0.16 x 100 =16 cats 84 cats Question 3 Sorry! That is incorrect. Try again! Question 3 Congratulations! You are correct! Practice with Hardy Weinberg Now we know how many of our cats have the BB genotype and the bb genotype. We can find the number of Bb cats using our equation: p2 + 2pq + q2 = 1. 2pq represents the proportion of cats with Bb. 2 x 0.6(p) x 0.4(q) = 0.48 0.48 x 100 = 48 cats with Bb genotype. Question 4 Let’s try another! In our population of 100 cats, 75 are black & 25 are white. Where do we start? 75 black cats = p2. 75/100 = 0.75 black cats = p2. 25 white cats = q2. 25/100 = 0.25 white cats = q2. Need more information. Question 4 Sorry! That is incorrect. Try again! Question 4 Congratulations! You are correct! Question 5 If q2 = 0.25, q= 0.05 5 0.5 50 Question 5 Sorry! That is incorrect. Try again! Question 5 Congratulations! You are correct! Question 6 If q=0.5, p= 0.5 5 0.6 0.1 Question 6 Sorry! That is incorrect. Try again! Question 6 Congratulations! You are correct! Question 7 So, if p=0.5, and p2=0.25, how many of our cats have the BB genotype? 0.25 25 50 75 Question 7 Sorry! That is incorrect. Try again! Question 7 Congratulations! You are correct! Question 8 Now, how many of the cats are heterozygous (Bb)? 48 100 .5 50 Question 8 Sorry! That is incorrect. Try again! Question 8 Congratulations! You are correct! Question 9 If we measure allele frequency one year at p=0.8 & q=0.2 and then go back 5 generations later to find p=0.5 & q=0.5, what has happened? The population has remained in Hardy-Weinberg equilibrium. The population has doubled in size. There has been a change in allele frequencies: evolution has occurred. Nothing has changed. Question 9 Sorry! That is incorrect. Try again! Question 9 Congratulations! You are correct!
