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Chapter 3 (part 2)
Chapter 3 (part 2)

... cleaves at COOH end of Lys and Arg cleaves at COOH end of Phe, Tyr, Trp ...
Data Analysis
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... By a graph we mean a representation of numerical values or functions by the positions of points and lines on a two-dimensional surface. A graph is inherently more limited in precision than a table of numerical values or an analytic equation, but it can contribute a "feel'' for the behavior of data a ...
Separation and Isolation of Plant Constituents
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... solute to matrix then recovering one bound species at a time • Conditions (pH, ionic strength) required to liberate species are determined by electrical properties ...
Year 12 AS Biology Module 1: Biological Molecules Name: PAPER
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... Calculate the Rf value of spot X. Show your working. ...
Hydrolysis of Aspartame
Hydrolysis of Aspartame

... contents to cool and label it hydrolysed aspartame. For the chromatography: With a pencil lightly draw a line along one end of the chromatography paper. Number each column, and write your name on the top. Using gloves carefully spot the following solutions on the relevant column: ...
Paper chromatography
Paper chromatography

... will be attracted or stick more to it but non-polar components will move across the surface easily.  If the stationary phase is nonpolar then nonpolar components will be more attracted to it and the polar ...
lab-6-chrmatography
lab-6-chrmatography

... attracted differently to the porous stationary phase depending on its polarity and the polarity of the stationary phase chosen.  Remember that “Like attracts Like”.  If the stationary phase is polar then polar components will be attracted or stick more to it but non-polar components will move acro ...
dana
dana

... GFAAS was studied. The optimum temperatures for pyrolysis and atomization for tin estimation were 1100ºC and 2300ºC, respectively. When different matrix modifiers were used, like a mixture of NH4H2PO4/Mg (NO3)2 and Mg (NO3)2 alone exhibited the optimum signal for tin. No remarkable effect on the tin ...
Affinity Chromatography using fusion proteins
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... will be attracted or stick more to it but non-polar components will move across the surface easily.  If the stationary phase is nonpolar then nonpolar components will be more attracted to it and the polar ...
Organic Acids - The Nest Group, Inc.
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... Organic acids are hydrophilic compounds with acidic properties where the carboxylic acids are predominant. Organic acids are generally weak acids that do not dissociate completely in water and they are present in every meal we eat. Organic acids are also used in food preservation because they can pe ...
Protein Purification and Characterization Techniques
Protein Purification and Characterization Techniques

... protein molecule and enhance the polarity of water, thus attracting protein into the solution and enhancing the solubility of protein • Ammonium sulfate is the most common reagent to use at this step • This technique is important but results are crude ...
Chromatography
Chromatography

... In the ascending method, the solvent is kept at the base of the chamber & the edges of the paper where the sample has been applied is immersed in the solvent taking care that the sample spots do not get dipped in the solvent but remain just above the surface of the solvent. The solvent moves up or a ...
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... recently isolated and reported, but initial structural studies and sequence homology suggests that it is part of a two-component bacteriocin. To test this hypothesis, the proposed second component CbnY, a 33 amino acid peptide predicted by genetics, was prepared by both solid phase peptide synthesis ...
Protein Purification and Characterization Techniques
Protein Purification and Characterization Techniques

... • Stationary: Samples interacts with this phase • Mobile: Flows over the stationary phase and carries along with it the sample to be separated ...
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... 1.16891 / 1.16886 Fractogel® EMD COO- (S), (M) Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and che ...
centennial honors college - Western Illinois University
centennial honors college - Western Illinois University

Ion exchange chromatography
Ion exchange chromatography

... Chromatography is separation method based on different retention of analytes on the stationary phase. Different chemical of physical phenomena can be used to retain an analyte. Depending on the phenomenon responsible for separation several types of chromatography are considered. Types of chromatogr ...
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Chromatography



Chromatography (/ˌkroʊməˈtɒɡrəfi/; from Greek χρῶμα chroma which means ""color"" and γράφειν graphein ""to write"") is the collective term for a set of laboratory techniques for the separation of mixtures.The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus changing the separation.Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.
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