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E1. A codon contains three nucleotides. Since G and C are present
E1. A codon contains three nucleotides. Since G and C are present

... be a down promoter mutation (i.e., a mutation in the promoter that decreases the rate of transcription), or it could be a mutation in the coding sequence that causes the protein to be degraded very rapidly. The sample in lane 4 came from an individual who is homozygous for a mutation that changed on ...
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DNA - Paxon Biology

... - The mRNA made in transcription leaves the nucleus and then travels into the cytoplasm to be translated. - Translation occurs on the ribosomes. - Ribosomes are made of rRNA - Ribosomes facilitate the orderly linking of amino acids into polypeptide chains. - During translation, the linear sequence o ...
Operon Info_pGLO pre lab
Operon Info_pGLO pre lab

... binding to the operator and blocking RNA polymerase  The repressor is the product of a separate regulatory gene  Repressors are often made continuously by the cell, at low levels © 2014 Pearson Education, Inc. ...
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... • Structural genes: encoding proteins • Regulatory genes: encoding products that interact with other sequences and affect the transcription and translation of these sequences • Regulatory elements: DNA sequences that are not transcribed but play a role in regulating other nucleotide sequences ...
Biological Sequences: DNA, RNA, Protein
Biological Sequences: DNA, RNA, Protein

... • deoxyribonucleic acid (DNA) Basic characteristics of DNA and RNA • DNA has only one biological role, but it is a central one. The information to make all the functional macromolecules of an organism (even DNA itself) is preserved in DNA and accessed through transcription of the information into RN ...
Gene Section ATF2 (activating transcription factor 2) Atlas of Genetics and Cytogenetics
Gene Section ATF2 (activating transcription factor 2) Atlas of Genetics and Cytogenetics

... The EWS-ATF2 fusion protein retains the ATF2 Cterminal region that contains the bZIP dimerization domain. But the fusion protein has lost the N-terminal domain of ATF2 that is kinase inducible. The Nterminal region of EWS is retained in the fusion protein but has lost both its RNA binding domain and ...
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biological sciences 354
biological sciences 354

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... bonds anymore – they can now pair with free individual nucleotides present in the nucleus (C≡G & A=T) one at a time & form new hydrogen bonds with the old strand (= the template). • The enzyme DNA-polymerase checks if the pairing of bases is correct & joints the new bases to a new backbone (catalyze ...
C2005/F2401 `09
C2005/F2401 `09

... but it is nonsense, not missense – it creates a premature stop codon. B-3. See the code table. The two correct choices are synonymous, although CGA to AGA doesn’t look it at first. (Note that the ability to use the same tRNA or a different one is not important here. That’s an issue of wobble, and th ...
manual HiScribe T7 In Vitro Transcription Kit E2030
manual HiScribe T7 In Vitro Transcription Kit E2030

... purification. For unpurified, heat-killed restriction digests, include no more than 13 µl of template per 40 µl reaction. For unpurified PCR product, include no more than 7 µl per 40 µl reaction. In all cases, the amount of added template DNA should not exceed 2 µg per 40 µl reaction, as RNA yields ...
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... stress responses, and so creates mutations when the organism is maladapted to the environment. SIM mechanisms occur in bacterial, yeast and human cells, and produce genetic diversity. A network of 93 proteins promotes SIM, but the screen that identified these genes was biased against small genes inc ...
Eukaryotic Gene Regulation | Principles of Biology from Nature
Eukaryotic Gene Regulation | Principles of Biology from Nature

... changes. In eukaryotes, some mRNAs may exist for periods ranging from days to weeks, and they may be repeatedly translated, such as the mRNAs that produce hemoglobin molecules in red blood cells. mRNA stability seems to be associated with changes in the poly(A) tail length. If the tail is shortened, ...
Exam 2a - web.biosci.utexas.edu
Exam 2a - web.biosci.utexas.edu

... 20. (2 points) True / False In the tryptophan operon (a repressible system under negative control) the repressor is active until it is bound to tryptophan (the co-repressor). 21. (4 points) Molecular chaperones such as Dna K and Dna J are found in higher amounts in an E. coli culture growing at 42  ...
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DNA, RNA, and Proteins

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Transcription & Translation

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4. Protein Synthesis and Biotechnology

... subunits: a five-carbon pentose sugar, a phosphoric acid group, and one of four nitrogen bases. (For DNA these nitrogen bases are adenine, guanine, cytosine, or thymine.) DNA and RNA differ in a number of major ways. A DNA nucleotide contains a deoxyribose sugar, but RNA contains ribose sugar. The n ...
Transcription Factors (from Wray et al Mol Biol Evol 20:1377)
Transcription Factors (from Wray et al Mol Biol Evol 20:1377)

...  Complexed with action of other genes • Size varies • Structural features vary • Encode for a protein(s) that is translated from a mRNA • Expression o Requires many associated factors ...
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Non-coding RNA



A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.
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