Microbial Ecology: Where are we now?
... DNA, using either primers or probes each of which have been demonstrated to have unique NGS application. Target enrichment techniques are evaluated in terms of multiplexity, specificity, uniformity, drop-off rate and reproducibility. The above-mentioned techniques were all evaluated using these key ...
... DNA, using either primers or probes each of which have been demonstrated to have unique NGS application. Target enrichment techniques are evaluated in terms of multiplexity, specificity, uniformity, drop-off rate and reproducibility. The above-mentioned techniques were all evaluated using these key ...
VegT activates Bix4 to specify endodermal
... Bix4-LUC construct expressed reporter genes in both mesoderm and endoderm, in a pattern resembling that of endogenous Bix4 (Fig. 3B; Table 1). Interestingly, vegetal expression of the Bix4-GFP construct was higher than that of Bix4-LUC. This may reflect a difference in RNA stability or in the abilit ...
... Bix4-LUC construct expressed reporter genes in both mesoderm and endoderm, in a pattern resembling that of endogenous Bix4 (Fig. 3B; Table 1). Interestingly, vegetal expression of the Bix4-GFP construct was higher than that of Bix4-LUC. This may reflect a difference in RNA stability or in the abilit ...
Biophysics 101 Genomics and Computational Biology
... A U1 small nuclear ribonucleoprotein particle with altered specificity induces alternative splicing of an adenovirus E1A mRNA precursor. Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations. An altered specificity mutation in the lambda repr ...
... A U1 small nuclear ribonucleoprotein particle with altered specificity induces alternative splicing of an adenovirus E1A mRNA precursor. Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations. An altered specificity mutation in the lambda repr ...
Part III: Laboratory – Electrophoresis
... and thus showing the mutant phenotype, will be used for mapping. Since both of their chromosomes contain the mutation and the mutation was from a Ler background, the number of crossing-over events is equivalent to the number of times the Col ecotype is found on the chromosome. There are many DNA seq ...
... and thus showing the mutant phenotype, will be used for mapping. Since both of their chromosomes contain the mutation and the mutation was from a Ler background, the number of crossing-over events is equivalent to the number of times the Col ecotype is found on the chromosome. There are many DNA seq ...
Genomic rearrangements account for more than one
... patients B212 and B242 more than one aberrant PCR product was co-amplified together with the wild-type transcript (Fig. 3A); sequence analysis of the gel purified bands revealed transcripts missing those exons that showed hemizygosity in the MLPA analysis as well as transcripts that lacked exons oth ...
... patients B212 and B242 more than one aberrant PCR product was co-amplified together with the wild-type transcript (Fig. 3A); sequence analysis of the gel purified bands revealed transcripts missing those exons that showed hemizygosity in the MLPA analysis as well as transcripts that lacked exons oth ...
Trichothiodystrophy: Update on the sulfur
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
... of the XPD gene. Rarely, mutated XPB gene or an unidentified TTD-A gene may result in TTD. In UVsensitive TTD, the TFIIH transcription factor containing XPB and XPD helicase activities necessary for both transcription initiation and DNA repair is damaged. Beyond deficiency in the NER pathway, it is ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
Oliver, B., Kim, Y.-J., and Baker, B. S.
... this end, our initial experiments address whether the sexspecific functioning of Sxl in the germ line is regulated by alternative pre-mRNA processing as it is in the soma. A finding that Sxl+ produces sex-specific transcripts within the germ line enables us to use these sex-specific transcripts as a ...
... this end, our initial experiments address whether the sexspecific functioning of Sxl in the germ line is regulated by alternative pre-mRNA processing as it is in the soma. A finding that Sxl+ produces sex-specific transcripts within the germ line enables us to use these sex-specific transcripts as a ...
Obligate phototrophy in cyanobacteria: more than a lack of sugar
... distinct characteristics. In P. boryanum uptake is inducible by glucose, and the glucose analogue Kmethyl-glucoside shows high competitive e¡ect rather than OMG in Synechocystis [6^8]. The transport system has no a¤nity for fructose, which is transported via a distinct system and also used as a subs ...
... distinct characteristics. In P. boryanum uptake is inducible by glucose, and the glucose analogue Kmethyl-glucoside shows high competitive e¡ect rather than OMG in Synechocystis [6^8]. The transport system has no a¤nity for fructose, which is transported via a distinct system and also used as a subs ...
Investigating the DNA-Binding Interactions of Small
... Theodore Goodson III for the opportunity. He is one of the few people who has stood up and fought for me. Without him, this would have not been possible. He has provided me the guidance, support, and resources to be successful as a scientist. I will be forever thankful for Prof. Goodson. He will be ...
... Theodore Goodson III for the opportunity. He is one of the few people who has stood up and fought for me. Without him, this would have not been possible. He has provided me the guidance, support, and resources to be successful as a scientist. I will be forever thankful for Prof. Goodson. He will be ...
The Nature of Life – Chapter 1
... Objectives: 8.2.1: Explain what experiments reveal about how plants grow. (brief) 8.2.2: State the overall equation for photosynthesis. 8.2.3: Describe the role of light and chlorophyll in photosynthesis. Objectives: 8:3:1: Describe the structure and function of a chloroplast. 8:3:2: Describe what h ...
... Objectives: 8.2.1: Explain what experiments reveal about how plants grow. (brief) 8.2.2: State the overall equation for photosynthesis. 8.2.3: Describe the role of light and chlorophyll in photosynthesis. Objectives: 8:3:1: Describe the structure and function of a chloroplast. 8:3:2: Describe what h ...
NUCLEIC ACID ECONOMY IN BACTERIA INFECTED WITH
... phage DNA (Hershey, Dixon, and Chase, 1953). Second, one can measure the DNA-P synthesized after infection (or DNA-P assimilated after infection, which turns out to be the same thing), add to it an allowance for the bacterial DNA that has disappeared (since an equivalent amount of phosphorus enters ...
... phage DNA (Hershey, Dixon, and Chase, 1953). Second, one can measure the DNA-P synthesized after infection (or DNA-P assimilated after infection, which turns out to be the same thing), add to it an allowance for the bacterial DNA that has disappeared (since an equivalent amount of phosphorus enters ...
Gene Section PAX8 (paired box 8) Atlas of Genetics and Cytogenetics
... follicular cells. PPARgamma is a ligand dependent nuclear transcription factor highly expressed in adipose tissue. The PAX8 promoter, which is active in thyroid follicular cells, drives the expression of PAX8/ PPARgamma1 fusion protein (PPFP). Since no point mutations in the PPARgamma1 gene have bee ...
... follicular cells. PPARgamma is a ligand dependent nuclear transcription factor highly expressed in adipose tissue. The PAX8 promoter, which is active in thyroid follicular cells, drives the expression of PAX8/ PPARgamma1 fusion protein (PPFP). Since no point mutations in the PPARgamma1 gene have bee ...
genetics - Liceocopernico.it
... Some exposure to college-level or university-level biology is desirable before embarking on the study of genetics. In this volume, however, basic biological principles (such as cell structures and functions) are reviewed to provide a common base of essential background information. The quantitative ...
... Some exposure to college-level or university-level biology is desirable before embarking on the study of genetics. In this volume, however, basic biological principles (such as cell structures and functions) are reviewed to provide a common base of essential background information. The quantitative ...
Bouquet Ears in Corn
... hormones and causes the plant to allocate resources to a primary ear. Hence, even though several potential ear shoots may have developed at V5, only one often produces a harvestable ear. Bouquet ears are often at the node below where the primary ear would be. This indicates that stress at V6 or V7 l ...
... hormones and causes the plant to allocate resources to a primary ear. Hence, even though several potential ear shoots may have developed at V5, only one often produces a harvestable ear. Bouquet ears are often at the node below where the primary ear would be. This indicates that stress at V6 or V7 l ...
Charge Transport in DNA - Insights from
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
Alu - Environmental
... • The LINE (Long INterspersed Element) L1 supplies the reverse transcriptase needed for Alu to jump • L1 is a defective retrovirus • It nicks the DNA close to the sequence AATTTT • The AAAA of Alu transcript binds to the TTTT of the nicked DNA – the acts as a primer for the L1 to bind to ...
... • The LINE (Long INterspersed Element) L1 supplies the reverse transcriptase needed for Alu to jump • L1 is a defective retrovirus • It nicks the DNA close to the sequence AATTTT • The AAAA of Alu transcript binds to the TTTT of the nicked DNA – the acts as a primer for the L1 to bind to ...
Attachment 2.2 Sequencing results
... fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and investigating whether there is more than just one transcript of the AHR gene in t ...
... fecundity and are defective in development of both liver and immune system. In these mice fetal vascular structures are found in the eyes, kidneys and liver. [1] Objective: to study the conservation of the AHR gene and investigating whether there is more than just one transcript of the AHR gene in t ...
Graph-based Methods for the Design of DNA - TUBdok
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
... DNA computing is a rapidly evolving field utilizing DNA molecules instead of silicon-based electronic units to perform calculations. The reliability of such computations strongly depends on the DNA sequences that represent units of information. Recently, the thermodynamic constraints, based on the f ...
view - Association for Computational Linguistics
... DNA provides instructions to a living cell to produce a protein. DNA has a double helix structure (reminiscent of a twisted ladder) and is made up of four nucleotides: adenine, cytosine, guanine, and thymine, commonly abbreviated as A, T, C, and G. Each nucleotide always appears paired with another ...
... DNA provides instructions to a living cell to produce a protein. DNA has a double helix structure (reminiscent of a twisted ladder) and is made up of four nucleotides: adenine, cytosine, guanine, and thymine, commonly abbreviated as A, T, C, and G. Each nucleotide always appears paired with another ...
Alu Human Polymorphism
... Human Genome • Other items in genome – Introns – Repeated sequences (telomeres, centromeres) – Non-coding RNAs – Transposons (SINES and LINES) (Short Interspersed Elements: less than 500 bp long and present 500,000 or more in the genome and Long Interspersed Elements) – Psuedogenes (genes that no ...
... Human Genome • Other items in genome – Introns – Repeated sequences (telomeres, centromeres) – Non-coding RNAs – Transposons (SINES and LINES) (Short Interspersed Elements: less than 500 bp long and present 500,000 or more in the genome and Long Interspersed Elements) – Psuedogenes (genes that no ...
CTLA-4 gene expression is influenced by promoter and
... We found no genotype-related difference in intracellular CTLA-4 protein expression. Moreover, the observed relationship between cell-surface expression of the CTLA-4 and specific genotypes was confined to unsorted cells and certain T-cell phenotypes. One explanation for this would be that mechanisms ...
... We found no genotype-related difference in intracellular CTLA-4 protein expression. Moreover, the observed relationship between cell-surface expression of the CTLA-4 and specific genotypes was confined to unsorted cells and certain T-cell phenotypes. One explanation for this would be that mechanisms ...
8. Principles of Genetics and Cytogenetics
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
... Historical background of genetics, theories and hypothesis. Physical basis of heredity, cell reproduction, mitosis, meiosis and its significance. Gametogenesis and syngamy in plants. Mendelian genetics–Mendel’s principles of heredity, deviation from Mendelian inheritance, pleiotropy, threshold chara ...
Table of Contents - Scholars` Bank
... By this time, the codon-anticodon interactions were thought to be standard Watson-Crick base pairing; hydrogen bonding that binds the trinucleotide anticodon to its complementary trinucleotide codon. Due to such bonding, many thought that since there were 64 codons, there would also be 64 correspond ...
... By this time, the codon-anticodon interactions were thought to be standard Watson-Crick base pairing; hydrogen bonding that binds the trinucleotide anticodon to its complementary trinucleotide codon. Due to such bonding, many thought that since there were 64 codons, there would also be 64 correspond ...
MA112 Expresso® Rhamnose SUMO Cloning and
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
... is designed for expression in E. coli, fusion clones constructed using this system can also be transferred into eukaryotic expression vectors for expression of SUMO-tagged proteins in mammalian or insect cells. The rhaP BAD promoter is a versatile tool for protein expression. In the absence of rhamn ...
Primary transcript
A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs. The primary transcripts designated to be mRNAs are modified in preparation for translation. For example, a precursor messenger RNA (pre-mRNA) is a type of primary transcript that becomes a messenger RNA (mRNA) after processing.There are several steps contributing to the production of primary transcripts. All these steps involve a series of interactions to initiate and complete the transcription of DNA in the nucleus of eukaryotes. Certain factors play key roles in the activation and inhibition of transcription, where they regulate primary transcript production. Transcription produces primary transcripts that are further modified by several processes. These processes include the 5' cap, 3'-polyadenylation, and alternative splicing. In particular, alternative splicing directly contributes to the diversity of mRNA found in cells. The modifications of primary transcripts have been further studied in research seeking greater knowledge of the role and significance of these transcripts. Experimental studies based on molecular changes to primary transcripts the processes before and after transcription have led to greater understanding of diseases involving primary transcripts.