Biomedical Imaging and Applied Optics Laboratory
... In the last 40 years, many tomographic imaging modalities have been developed such as Computed Tomography (CT), Magnetic Resonance Imaging (MRI), Ultrasound (US), and Positron Emission Tomography (PET). Optical coherence tomography (OCT) is a relatively recent imaging technology for producing high-r ...
... In the last 40 years, many tomographic imaging modalities have been developed such as Computed Tomography (CT), Magnetic Resonance Imaging (MRI), Ultrasound (US), and Positron Emission Tomography (PET). Optical coherence tomography (OCT) is a relatively recent imaging technology for producing high-r ...
High-performance holographic technologies for fluid
... nearly canonical flows, whereas there is a substantial need, in both practical applications and validation of new theoretical approaches and large-scale numerical simulations, to understand complex turbulent flows under realistic conditions, including multi-phase, buoyancy-driven and high Reynolds num ...
... nearly canonical flows, whereas there is a substantial need, in both practical applications and validation of new theoretical approaches and large-scale numerical simulations, to understand complex turbulent flows under realistic conditions, including multi-phase, buoyancy-driven and high Reynolds num ...
Spectral phase conjugation via temporal imaging
... provide more extensive dispersion compensation performance [4]. Specifically, SPC offers the potential to compensate for all orders of dispersion and some of the nonlinear effects in optical fibers such as self-phase modulation (SPM) and self steepening (SS), whereas TPC can compensate only for even ...
... provide more extensive dispersion compensation performance [4]. Specifically, SPC offers the potential to compensate for all orders of dispersion and some of the nonlinear effects in optical fibers such as self-phase modulation (SPM) and self steepening (SS), whereas TPC can compensate only for even ...
High-Resolution Retinal Imaging using Adaptive Optics in a
... how each individual retinal component contributes to vision. The improvement in imaging systems permits the imaging of smaller retinal structures and thinner layers, and therefore helps in determining what the functions of individual types of cells are, in monitoring the blood flow in the retina eve ...
... how each individual retinal component contributes to vision. The improvement in imaging systems permits the imaging of smaller retinal structures and thinner layers, and therefore helps in determining what the functions of individual types of cells are, in monitoring the blood flow in the retina eve ...
Polarization microscopy with the LC-PolScope
... was dominated by techniques such as phase contrast, differential interference contrast and polarized light microscopy which do not require the introduction of exogenous dyes or markers. Fluorescence microscopy, on the other hand, often required the killing and fixation of cells and then labeling the ...
... was dominated by techniques such as phase contrast, differential interference contrast and polarized light microscopy which do not require the introduction of exogenous dyes or markers. Fluorescence microscopy, on the other hand, often required the killing and fixation of cells and then labeling the ...
Imaging Properties of Laser-Produced Parabolic Profile Microlenses
... cross-section, and the spot diagram. Those are standard means of determination of image quality when aberrations are small and optical system is the diffraction limited system. Before starting any image analysis it is important to mention that five concave microlenses that are analysed are quite dif ...
... cross-section, and the spot diagram. Those are standard means of determination of image quality when aberrations are small and optical system is the diffraction limited system. Before starting any image analysis it is important to mention that five concave microlenses that are analysed are quite dif ...
Digital Radiographic Image Acquisition and Processing
... Laser stands for light amplification by stimulated emission of radiation. Laser creates and amplifies a narrow, intense beam of coherent light. Atoms or molecules of a crystal such as a gas, liquid, or substances such as ruby or garnet are excited so that more of them are at high energy levels rathe ...
... Laser stands for light amplification by stimulated emission of radiation. Laser creates and amplifies a narrow, intense beam of coherent light. Atoms or molecules of a crystal such as a gas, liquid, or substances such as ruby or garnet are excited so that more of them are at high energy levels rathe ...
A Brief History of the Microscope and its Significance
... Light sources for confocal microscopes can be divided into spatially coherent or spatially incoherent groups. LSCMs are spatially coherent. In contrast, many of the clinical confocal microscopes used in the clinic, for example, the scanning-slit confocal microscope used in the ophthalmology clinic u ...
... Light sources for confocal microscopes can be divided into spatially coherent or spatially incoherent groups. LSCMs are spatially coherent. In contrast, many of the clinical confocal microscopes used in the clinic, for example, the scanning-slit confocal microscope used in the ophthalmology clinic u ...
super-resolved fluorescence microscopy
... technique remains technically cumbersome and its applications have remained limited to surface studies. The scientific and technical breakthrough in super-resolved fluorescence microscopy has instead come in the form of far-field excitation techniques of two main types, as will be described in the ...
... technique remains technically cumbersome and its applications have remained limited to surface studies. The scientific and technical breakthrough in super-resolved fluorescence microscopy has instead come in the form of far-field excitation techniques of two main types, as will be described in the ...
S U P E R -R E S O LV... Scientific Background on the Nobel Prize in Chemistry 2014
... technique remains technically cumbersome and its applications have remained limited to surface studies. The scientific and technical breakthrough in super-resolved fluorescence microscopy has instead come in the form of far-field excitation techniques of two main types, as will be described in the ...
... technique remains technically cumbersome and its applications have remained limited to surface studies. The scientific and technical breakthrough in super-resolved fluorescence microscopy has instead come in the form of far-field excitation techniques of two main types, as will be described in the ...
Quantitative spectroscopic photoacoustic imaging
... PAT. There are essentially two modes of PA microscopy, AR-PAM and OR-PAM. In acoustic resolution PA microscopy (AR-PAM), a focused ultrasound detector is used to record the PA signal, and the axial and lateral spatial resolutions are limited by ultrasonic considerations. In a typical implementation, ...
... PAT. There are essentially two modes of PA microscopy, AR-PAM and OR-PAM. In acoustic resolution PA microscopy (AR-PAM), a focused ultrasound detector is used to record the PA signal, and the axial and lateral spatial resolutions are limited by ultrasonic considerations. In a typical implementation, ...
Heidelberg Retina Tomograph - HRT
... the focal plane is focused to the pinhole, can pass it and is detected. However, light reflected from layers of the three-dimensional object above or below the focal plane is not focused to the pinhole, and only a small fraction of it can pass the pinhole and is detected. Therefore, the out-of-focus ...
... the focal plane is focused to the pinhole, can pass it and is detected. However, light reflected from layers of the three-dimensional object above or below the focal plane is not focused to the pinhole, and only a small fraction of it can pass the pinhole and is detected. Therefore, the out-of-focus ...
Immersion microscopy based on photonic crystal materials
... produced originally by surface plasmon polaritons in the metal surface plane is observed with a conventional optical microscope due to SPP coupling to light via random surface roughness and nanotailored surface of the periodic nanohole array. Glycerin microdroplets have been used as 2D magnifying mi ...
... produced originally by surface plasmon polaritons in the metal surface plane is observed with a conventional optical microscope due to SPP coupling to light via random surface roughness and nanotailored surface of the periodic nanohole array. Glycerin microdroplets have been used as 2D magnifying mi ...
Yu Chen
... specificity for biomedical diagnosis. Recent developments on specific-targeting fluorophores such as molecular beacons offer high contrast between normal and cancerous tissues, hence provide promising means for early tumor detection. In this work, we study the contrast enhancement by applying the du ...
... specificity for biomedical diagnosis. Recent developments on specific-targeting fluorophores such as molecular beacons offer high contrast between normal and cancerous tissues, hence provide promising means for early tumor detection. In this work, we study the contrast enhancement by applying the du ...
Fluorescence Microscopy
... fluoresces and emits photons in the red region. In conventional imaging, spatial resolution is dependent on the wavelength of the excitation light and the microscope optics. In practice, with one-photon confocal imaging, this yields lateral and axial resolution of 200 and 500 nm, respectively. Supe ...
... fluoresces and emits photons in the red region. In conventional imaging, spatial resolution is dependent on the wavelength of the excitation light and the microscope optics. In practice, with one-photon confocal imaging, this yields lateral and axial resolution of 200 and 500 nm, respectively. Supe ...
Jens Prescher, Establishment and Optimization of Super
... Optical microscopy as a non-invasive method even allows for the studying of living organisms and thereby the imaging of dynamic processes. Furthermore, it is a method that cannot only be applied to surfaces (like SEM) or very thin structures (like TEM), but makes it possible to study the entire volu ...
... Optical microscopy as a non-invasive method even allows for the studying of living organisms and thereby the imaging of dynamic processes. Furthermore, it is a method that cannot only be applied to surfaces (like SEM) or very thin structures (like TEM), but makes it possible to study the entire volu ...
Quantitative determination of chromophore concentrations from 2D
... Potential applications include studies of the oxygenation heterogeneity in vascular structures, such as those in tumors, while the ability to map and quantify targeted contrast agents may allow the study of disease processes at a molecular level [8]. An earlier study demonstrated the recovery of abs ...
... Potential applications include studies of the oxygenation heterogeneity in vascular structures, such as those in tumors, while the ability to map and quantify targeted contrast agents may allow the study of disease processes at a molecular level [8]. An earlier study demonstrated the recovery of abs ...
Aperture interference and the volumetric resolution of light field
... that certain angular measurements may be redundant. This dimensionality mismatch, or dimensionality gap [36, 24], suggests that there may be room to improve the spatial resolution of the LFM tomographic approach while preserving equivalent volumetric information [26]. In particular, by not limiting ...
... that certain angular measurements may be redundant. This dimensionality mismatch, or dimensionality gap [36, 24], suggests that there may be room to improve the spatial resolution of the LFM tomographic approach while preserving equivalent volumetric information [26]. In particular, by not limiting ...
Quadriwave lateral shearing interferometry
... 27. A. Sergé, N. Bertaux, H. Rigneault and D. Marguet, ”Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes”, Nature Methods 5, 687–695 (2008). ...
... 27. A. Sergé, N. Bertaux, H. Rigneault and D. Marguet, ”Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes”, Nature Methods 5, 687–695 (2008). ...
Far-Field Optical Superlens
... It can be clearly seen that the FSL projects distinctive fringe images for each object (Figure 3a). The periodicity of the fringe images in the far field increases for objects with characteristic sizes that approach the period of the FSL grating (Λ ) 150 nm). This effect is similar to Moiré fringes ...
... It can be clearly seen that the FSL projects distinctive fringe images for each object (Figure 3a). The periodicity of the fringe images in the far field increases for objects with characteristic sizes that approach the period of the FSL grating (Λ ) 150 nm). This effect is similar to Moiré fringes ...
Optimization of axial resolution in a confocal microscope with
... of the pupils are decreased. However, in practice, a finite-sized pinhole is placed in front of the detector, and in practice the size of the pinhole is sometimes increased to raise the signal strength. Therefore, our aim in this paper is to investigate the confocal system with two centrosymmetrical ...
... of the pupils are decreased. However, in practice, a finite-sized pinhole is placed in front of the detector, and in practice the size of the pinhole is sometimes increased to raise the signal strength. Therefore, our aim in this paper is to investigate the confocal system with two centrosymmetrical ...
JBO Letters Towards noncontact skin melanoma selection by
... and 32 nonmalignant vascular lesions (hemangiomas—30, port-wine stains—2) were selected for a more detailed study, with special attention to melanomas as the most dangerous skin malformations. “Fading” of spectral images with increased wavelength was observed for three skin pathologies: melanomas, n ...
... and 32 nonmalignant vascular lesions (hemangiomas—30, port-wine stains—2) were selected for a more detailed study, with special attention to melanomas as the most dangerous skin malformations. “Fading” of spectral images with increased wavelength was observed for three skin pathologies: melanomas, n ...
4Pi Microscopy
... properties of the dye to break the diffraction barrier. Using two lenses for STED, the axial resolution was improved down to 30 to 50 nm (Dyba and Hell, 2002; Dyba et al., 2003). However, as STED is still in its infancy and, unlike I5M and 4Pi microscopy, relies on the specific properties of the dye ...
... properties of the dye to break the diffraction barrier. Using two lenses for STED, the axial resolution was improved down to 30 to 50 nm (Dyba and Hell, 2002; Dyba et al., 2003). However, as STED is still in its infancy and, unlike I5M and 4Pi microscopy, relies on the specific properties of the dye ...
Biomedical Optical Imaging
... scan cameras are available and enable spectral OCT at longer wavelengths, these cameras have limited numbers of pixels and are relatively expensive. Swept source OCT can work at longer wavelengths, such as 1050 nm or 1300 nm, which are beyond the range of conventional line scan cameras. Imaging at l ...
... scan cameras are available and enable spectral OCT at longer wavelengths, these cameras have limited numbers of pixels and are relatively expensive. Swept source OCT can work at longer wavelengths, such as 1050 nm or 1300 nm, which are beyond the range of conventional line scan cameras. Imaging at l ...