xia immune activation 1
... and NKT cells, are also activated in other inflamed organs, such as the liver, to contribute indirectly to the atherosclerosis by increasing systemic inflammation and metabolic dysfunction. However, molecular events that link activation of different immune cells and abnormal metabolic conditions in ...
... and NKT cells, are also activated in other inflamed organs, such as the liver, to contribute indirectly to the atherosclerosis by increasing systemic inflammation and metabolic dysfunction. However, molecular events that link activation of different immune cells and abnormal metabolic conditions in ...
Liver-directed Gene Transfer and Prolonged Expression of Three
... ruses incapable of replication at 398C (23). The three plasmids, pAdCMVE2, pAdCMVE3, and pAdCMVE4, were linearized with NheI and cotransfected into 293 cells along with adenoviral DNA digested with ClaI, and cells were overlaid with agar and incubated at 328C for 15 d. Plaques were picked and screen ...
... ruses incapable of replication at 398C (23). The three plasmids, pAdCMVE2, pAdCMVE3, and pAdCMVE4, were linearized with NheI and cotransfected into 293 cells along with adenoviral DNA digested with ClaI, and cells were overlaid with agar and incubated at 328C for 15 d. Plaques were picked and screen ...
Rhesus macaque and mouse models for down
... for advancing novel vaccine candidates to human trials. The rodent model for malaria is based on Plasmodium berghei, Plasmodium chabaudi or Plasmodium yoelii parasites that naturally infect African thicket rats [2]. These parasites have been adapted to grow in mouse strains for routine laboratory ex ...
... for advancing novel vaccine candidates to human trials. The rodent model for malaria is based on Plasmodium berghei, Plasmodium chabaudi or Plasmodium yoelii parasites that naturally infect African thicket rats [2]. These parasites have been adapted to grow in mouse strains for routine laboratory ex ...
Fc lessons from murine mercury-induced autoimmunity. -receptors in systemic autoimmune conditions -
... heritability for most of the autoimmune diseases in monozygotic twins is less than 50%, indicating that the induction of autoimmunity must be influenced by environmental factors as well [2]. This has been shown to be the case with environmental factors like infections, drugs, toxic oils, pristane an ...
... heritability for most of the autoimmune diseases in monozygotic twins is less than 50%, indicating that the induction of autoimmunity must be influenced by environmental factors as well [2]. This has been shown to be the case with environmental factors like infections, drugs, toxic oils, pristane an ...
Apoptotic Debris Accumulates on Hematopoietic Cells and
... (16, 17). Promoter polymorphisms that reduce FcgRIIB expression on germinal center and activated B cells are associated with murine and human SLE (18, 19). In addition, mice lacking FcgRIIB (20) develop lupus-like disease. Other functional polymorphisms in human FcgRIIa (R/H131) and FcgRIIIa (158V/F ...
... (16, 17). Promoter polymorphisms that reduce FcgRIIB expression on germinal center and activated B cells are associated with murine and human SLE (18, 19). In addition, mice lacking FcgRIIB (20) develop lupus-like disease. Other functional polymorphisms in human FcgRIIa (R/H131) and FcgRIIIa (158V/F ...
Bacillus anthracis Protective Antigen Bound by Neutralizing Antibodies *
... phosphate-buffered saline; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe- ...
... phosphate-buffered saline; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe- ...
No Slide Title - Mercury Science
... assay, and the shipping and processing times involved. What is needed to address this issue is a means of directly testing for saxitoxins using an inexpensive, rapid field test. ...
... assay, and the shipping and processing times involved. What is needed to address this issue is a means of directly testing for saxitoxins using an inexpensive, rapid field test. ...
Distinct and Separable Roles of the Complement System in Factor H
... platelet and not other blood cells, such as the erythrocyte, despite their continuous exposure to plasma proteins (5). By analogy to erythrocyte CR1, we considered it possible that platelets are endowed with Cfh upon exit from the bone marrow. To investigate this, we cultured megakaryocytes from nor ...
... platelet and not other blood cells, such as the erythrocyte, despite their continuous exposure to plasma proteins (5). By analogy to erythrocyte CR1, we considered it possible that platelets are endowed with Cfh upon exit from the bone marrow. To investigate this, we cultured megakaryocytes from nor ...
Brucella melitensis with Immune Response upon Secondary
... vaccines for protection against brucellosis (19, 30, 31), suggesting that the localization and persistence of Brucella Ags are key factors in the development of protective immunity. However, there is no consensual explanation for this fundamental difference. The use of heat-killed (HK) preparations ...
... vaccines for protection against brucellosis (19, 30, 31), suggesting that the localization and persistence of Brucella Ags are key factors in the development of protective immunity. However, there is no consensual explanation for this fundamental difference. The use of heat-killed (HK) preparations ...
Pathogen, Subjects, Urinary Tract Infection
... specimens were collected from in and out patients from a general hospital in Port Harcourt. Some of the patients were on admission while more of them were out patients. Universal sterile containers were issued to patients and instructions on how to collect urine specimen was passed to them. Universa ...
... specimens were collected from in and out patients from a general hospital in Port Harcourt. Some of the patients were on admission while more of them were out patients. Universal sterile containers were issued to patients and instructions on how to collect urine specimen was passed to them. Universa ...
Lack of Chromatin and Nuclear Fragmentation In Vivo Impairs the
... Monestier, Michael Madaio, Minoru Satoh and Roberto Caricchio ...
... Monestier, Michael Madaio, Minoru Satoh and Roberto Caricchio ...
GM-CSF in the Lung Protects against Lethal
... on the pulmonary inflammatory response during influenza infection, we evaluated the effect of treating WT mice with GMCSF, using flow cytometry to measure cellular surface markers of inflammation. CD200R and CD200 are an anti-inflammatory ligand and receptor pair that are normally expressed at high ...
... on the pulmonary inflammatory response during influenza infection, we evaluated the effect of treating WT mice with GMCSF, using flow cytometry to measure cellular surface markers of inflammation. CD200R and CD200 are an anti-inflammatory ligand and receptor pair that are normally expressed at high ...
Candida
... from uninoculated mice (P,0.05 in each strain). No significant differences were detected in the levels of S100A8 or S100A9 between the four inoculated strains of mice (P.0.05). It was also important to confirm that the S100 alarmins were produced by vaginal epithelial cells in response to Candida si ...
... from uninoculated mice (P,0.05 in each strain). No significant differences were detected in the levels of S100A8 or S100A9 between the four inoculated strains of mice (P.0.05). It was also important to confirm that the S100 alarmins were produced by vaginal epithelial cells in response to Candida si ...
Study of PepT1 and SPAK on Intestinal Homeostasis and Intestinal
... crypt-villus axis demonstrated that this protein is crucial to the process of di/tripeptide absorption. We recently exhibited that PepT1 plays an important role in multiple biological functions, including the ability to regulate the expression/secretion of specific microRNAs (miRNAs) and the express ...
... crypt-villus axis demonstrated that this protein is crucial to the process of di/tripeptide absorption. We recently exhibited that PepT1 plays an important role in multiple biological functions, including the ability to regulate the expression/secretion of specific microRNAs (miRNAs) and the express ...
NOG Mice Transgenic − Using Human IL-3/GM
... this strain, a large number of human myeloid cells of various lineages developed after transplantation of human CD34+ hematopoietic stem cells. Notably, mature basophils and mast cells expressing Fc«RI were markedly increased. These humanized NOG IL-3/GM–Tg mice developed passive cutaneous anaphylax ...
... this strain, a large number of human myeloid cells of various lineages developed after transplantation of human CD34+ hematopoietic stem cells. Notably, mature basophils and mast cells expressing Fc«RI were markedly increased. These humanized NOG IL-3/GM–Tg mice developed passive cutaneous anaphylax ...
prolongation of allograft survival in ccr7
... major histocompatibility complex (MHC)-mismatched C3H (H-2k) hearts and skin were grafted to CCR7⫺/⫺ mice and to wild-type (WT) littermates (H-2b). Whereas WT mice rejected C3H hearts within 7.3 days, allograft survival in CCR7⫺/⫺ mice was prolonged to 10.7 days (P⬍0.01) (Table 1). Similarly, reject ...
... major histocompatibility complex (MHC)-mismatched C3H (H-2k) hearts and skin were grafted to CCR7⫺/⫺ mice and to wild-type (WT) littermates (H-2b). Whereas WT mice rejected C3H hearts within 7.3 days, allograft survival in CCR7⫺/⫺ mice was prolonged to 10.7 days (P⬍0.01) (Table 1). Similarly, reject ...
Plasmodium Strain Determines Dendritic Cell
... The severity of malaria can range from asymptomatic to lethal infections involving severe anaemia and cerebral disease. However, the molecular and cellular factors responsible for these differences in disease severity are poorly understood. Identifying the factors that mediate virulence will contrib ...
... The severity of malaria can range from asymptomatic to lethal infections involving severe anaemia and cerebral disease. However, the molecular and cellular factors responsible for these differences in disease severity are poorly understood. Identifying the factors that mediate virulence will contrib ...
Infectivity in extraneural tissues following intraocular scrapie infection
... at least three intracerebral (i.c.) passages at high dilution (described by Bruce, I996). Inocula were prepared as 10% homogenates in saline of brain tissue from terminally affected mice. Infection was introduced by one of five routes: i.c. (into right parietal cortex); i.o. (bilaterally into the vi ...
... at least three intracerebral (i.c.) passages at high dilution (described by Bruce, I996). Inocula were prepared as 10% homogenates in saline of brain tissue from terminally affected mice. Infection was introduced by one of five routes: i.c. (into right parietal cortex); i.o. (bilaterally into the vi ...
tuberculosis Mycobacterium Early Infection with Dynamic Roles of
... Although the protective role of type II IFN, or IFN-g, against Mycobacterium tuberculosis has been established, the effects of type I IFNs are still unclear. One potential confounding factor is the overlap of function between the two signaling pathways. We used mice carrying null mutations in the ty ...
... Although the protective role of type II IFN, or IFN-g, against Mycobacterium tuberculosis has been established, the effects of type I IFNs are still unclear. One potential confounding factor is the overlap of function between the two signaling pathways. We used mice carrying null mutations in the ty ...
Radioimmunotherapy Is More Effective than Antifungal Treatment
... protects C. neoformans from external g radiation (as reviewed by Dadachova and Casadevall [10]). We observed that melanization of C. neoformans before infection did not increase the resistance of C. neoformans to RIT in vivo. Possible explanations for this may include the delicate balance existing ...
... protects C. neoformans from external g radiation (as reviewed by Dadachova and Casadevall [10]). We observed that melanization of C. neoformans before infection did not increase the resistance of C. neoformans to RIT in vivo. Possible explanations for this may include the delicate balance existing ...
Immune Complex Deposits as a Characteristic Feature of
... diseases development (Tormey et al., 2001). In addition, a recent case-control study reported that severely affected scleroderma patients with anti-fibrillarin antibodies (AFA) were more likely to have higher levels of mercury in urine as compared either to less severely affected cases without AFA o ...
... diseases development (Tormey et al., 2001). In addition, a recent case-control study reported that severely affected scleroderma patients with anti-fibrillarin antibodies (AFA) were more likely to have higher levels of mercury in urine as compared either to less severely affected cases without AFA o ...
IMMUNOMODULATORY ACTIVITY OF ALCOHOLIC EXTRACT OF HABENARIA INTERMEDIA IN MICE Research Article
... Immunomodulatory agents that are free from side effects and which can be administered for long duration to obtain a continuous immune activation are highly desirable for the prevention of diseases. There a variety of naturally and chemically derived compound discovered with the Immunomodulatory acti ...
... Immunomodulatory agents that are free from side effects and which can be administered for long duration to obtain a continuous immune activation are highly desirable for the prevention of diseases. There a variety of naturally and chemically derived compound discovered with the Immunomodulatory acti ...
Characterization of cell-mediated immune responses elicited
... a single animal. The bar indicates mean ear swelling response for the group. Delayed hypersensitivity response of grafted mice was not significantly different from naive control mice. The next time at which DH was assessed in grafted mice was 4 weeks after keratoplasty. Based on our previous experie ...
... a single animal. The bar indicates mean ear swelling response for the group. Delayed hypersensitivity response of grafted mice was not significantly different from naive control mice. The next time at which DH was assessed in grafted mice was 4 weeks after keratoplasty. Based on our previous experie ...
Xenodiagnosis of Leishmania donovani in BALB/c mice using
... on human volunteers showed that Brazilian VL cases were infectious to feeding sand fly females while volunteers who were asymptomatic, were not [8]. However, in this study, asymptomatic infections were identified using LST that detects only late-stage cell-mediated immunity [9]. Hence, these LST pos ...
... on human volunteers showed that Brazilian VL cases were infectious to feeding sand fly females while volunteers who were asymptomatic, were not [8]. However, in this study, asymptomatic infections were identified using LST that detects only late-stage cell-mediated immunity [9]. Hence, these LST pos ...
R educed atherosclerosis in interleukin
... approximately the same extent. These findings suggest that IL-18 does not act on a single, specific step in lipoprotein conversion such as a lipase. Instead, it might modulate cholesterol uptake or synthesis. However, the effect of IL-18 on cholesterol metabolism requires further study. IL-18 defici ...
... approximately the same extent. These findings suggest that IL-18 does not act on a single, specific step in lipoprotein conversion such as a lipase. Instead, it might modulate cholesterol uptake or synthesis. However, the effect of IL-18 on cholesterol metabolism requires further study. IL-18 defici ...
Major urinary proteins
Major urinary proteins (Mups), also known as α2u-globulins, are a subfamily of proteins found in abundance in the urine and other secretions of many animals. Mups provide a small range of identifying information about the donor animal, when detected by the vomeronasal organ of the receiving animal. They belong to a larger family of proteins known as lipocalins. Mups are encoded by a cluster of genes, located adjacent to each other on a single stretch of DNA, that varies greatly in number between species: from at least 21 functional genes in mice to none in humans. Mup proteins form a characteristic glove shape, encompassing a ligand-binding pocket that accommodates specific small, organic chemicals.Urinary proteins were first reported in rodents in 1932, during studies by Thomas Addis into the cause of proteinuria. They are potent human allergens, and are largely responsible for a number of animal allergies, including to cats, horses and rodents. Their endogenous function within an animal is unknown, but may involve regulating energy expenditure. However, as secreted proteins they play multiple roles in chemical communication between animals, functioning as pheromone transporters and stabilizers in rodents and pigs. Mups can also act as protein pheromones themselves. They have been demonstrated to promote aggression in male mice, and one specific Mup protein found in male mouse urine is sexually attractive to female mice. Mups can also function as signals between different species: mice display an instinctive fear response on the detection of Mups derived from predators such as cats and rats.