The Functional Influence of Burst and Tonic Firing Mode on Synaptic
... Figure 1. Properties of synaptic connections between PGN and thalamocortical neurons. A, Schematic diagram of the recording arrangement for recordings in B–E is shown. The PGN neuron was activated via the intracellular injection of a depolarizing current pulse while recording from a recipient thalam ...
... Figure 1. Properties of synaptic connections between PGN and thalamocortical neurons. A, Schematic diagram of the recording arrangement for recordings in B–E is shown. The PGN neuron was activated via the intracellular injection of a depolarizing current pulse while recording from a recipient thalam ...
chronic lymphocytic leukemia (cll)
... greater than 5 G/L, with less than 55% of the cells being atypical 2) The cell should have the presence of Bcellspecific differentiation antigens (CD19, CD20, and CD24) and be CD5(+) 3) A bone marrow aspirates showing greater than 30% lymphocytes ...
... greater than 5 G/L, with less than 55% of the cells being atypical 2) The cell should have the presence of Bcellspecific differentiation antigens (CD19, CD20, and CD24) and be CD5(+) 3) A bone marrow aspirates showing greater than 30% lymphocytes ...
How to define flow cytometry ? Méthodes d’étude de la cellule
... Rappel PE-CY5 excited at 488 nm by the emit energy transferred from PE but also by the laser 633 nm via CY5, compensations could be elevated or difficult to realize so try to replace PE-CY5 by PECY5.5 or PE-CY7 in a combination with APC ...
... Rappel PE-CY5 excited at 488 nm by the emit energy transferred from PE but also by the laser 633 nm via CY5, compensations could be elevated or difficult to realize so try to replace PE-CY5 by PECY5.5 or PE-CY7 in a combination with APC ...
Taxonomic revision and species delimitation of coccoid green algae
... and the purified PCR products were ligated into the TOPOâ vector (TOPO TA Cloning Kit; Invitrogen, Karlsruhe, Germany) and cloned into competent E. coli cells (Top10; Invitrogen). For insert check, positive clones were directly used as template for PCR using the same primers and amplification condit ...
... and the purified PCR products were ligated into the TOPOâ vector (TOPO TA Cloning Kit; Invitrogen, Karlsruhe, Germany) and cloned into competent E. coli cells (Top10; Invitrogen). For insert check, positive clones were directly used as template for PCR using the same primers and amplification condit ...
Localisation of the Ki-67 antigen within the nucleolus
... antibody which reacts with A-type lamins in human cells (Bridger et al., 1993) was used at 1:500. S4 (anti-fibrillarin) and S18 (anti-RNA polymerase I), kind gifts from Prof. Dr Ulrich Scheer, University of Wurzburg, Germany, were used at 1:100. NPM (a kind gift from Dr Pui Chan, Baylor College of M ...
... antibody which reacts with A-type lamins in human cells (Bridger et al., 1993) was used at 1:500. S4 (anti-fibrillarin) and S18 (anti-RNA polymerase I), kind gifts from Prof. Dr Ulrich Scheer, University of Wurzburg, Germany, were used at 1:100. NPM (a kind gift from Dr Pui Chan, Baylor College of M ...
PDF
... originated near the anterior, lateral aspect of the ganglion and appeared to project processes both medially and laterally to form the AA segmental nerve. At ~60 hours clonal age, axon fibers projected laterally from the posterior aspect of the ganglion to establish the PP nerve. The appearance of t ...
... originated near the anterior, lateral aspect of the ganglion and appeared to project processes both medially and laterally to form the AA segmental nerve. At ~60 hours clonal age, axon fibers projected laterally from the posterior aspect of the ganglion to establish the PP nerve. The appearance of t ...
Hormonally Regulated Programmed Cell Death in
... numerous hydrolytic enzymes, including proteases and nucleases as well as a-amylases (Jones and Jacobsen, 1991). To determine whether cell death resulted from exposure to a lethal amount of hydrolase activities, we incubated protoplasts in hydrolase-containing media by using the procedure diagramed ...
... numerous hydrolytic enzymes, including proteases and nucleases as well as a-amylases (Jones and Jacobsen, 1991). To determine whether cell death resulted from exposure to a lethal amount of hydrolase activities, we incubated protoplasts in hydrolase-containing media by using the procedure diagramed ...
Differential Effects of Rapamycin on Mammalian Target of
... day until colonies were visible to the naked eye. Colonies were stained with plating 50,000 cells/ml in the presence or absence of 20 nM rapamycin and crystal violet (0.1% in 20% methanol). FL5.12 cells were maintained in RPMI measuring cell number at 24-h intervals with a Coulter Z2 particle analyz ...
... day until colonies were visible to the naked eye. Colonies were stained with plating 50,000 cells/ml in the presence or absence of 20 nM rapamycin and crystal violet (0.1% in 20% methanol). FL5.12 cells were maintained in RPMI measuring cell number at 24-h intervals with a Coulter Z2 particle analyz ...
Bioreactor landfills: experimental and field results
... distribution of the recirculated leachate. The nominal size of the gravel used for both filter layers range from 19 to 38 mm. Finally, the bioreactor cell covers were placed on top of each cell, and the whole unit was sealed using construction type sealant. The protruding ends of the gas collection w ...
... distribution of the recirculated leachate. The nominal size of the gravel used for both filter layers range from 19 to 38 mm. Finally, the bioreactor cell covers were placed on top of each cell, and the whole unit was sealed using construction type sealant. The protruding ends of the gas collection w ...
Collagen is the most abundant protein in the animal kingdom, and it
... basement membrane, a meshwork is formed that filters cells as well as molecules and light. For example, in the lens capsule of the eye, the basement membrane plays a role in light filtration. In the kidney, the glomerular basement membrane is responsible for filtration of the blood to remove waste p ...
... basement membrane, a meshwork is formed that filters cells as well as molecules and light. For example, in the lens capsule of the eye, the basement membrane plays a role in light filtration. In the kidney, the glomerular basement membrane is responsible for filtration of the blood to remove waste p ...
Osmotic Water Permeability of Isolated Protoplasts. Modifications
... gradient (Dc0) was created by filling compartments 1 and 19 and 2 and 29 with two solutions of different osmotic strengths. Capillary forces in the channel allow maintenance of an air gap between compartments 1 and 2 (Fig. 2, B and C) during filling operations. Communication between solutions was ac ...
... gradient (Dc0) was created by filling compartments 1 and 19 and 2 and 29 with two solutions of different osmotic strengths. Capillary forces in the channel allow maintenance of an air gap between compartments 1 and 2 (Fig. 2, B and C) during filling operations. Communication between solutions was ac ...
$doc.title
... tissues necessaries (embryonic and extraembryonic) to form the new organism when implanted at the uterus. This potential is contained in the genetic material inherited from the two gametes it arises from. Nevertheless, the uniqueness of the zygote lies in being the cell initiating the tight and prec ...
... tissues necessaries (embryonic and extraembryonic) to form the new organism when implanted at the uterus. This potential is contained in the genetic material inherited from the two gametes it arises from. Nevertheless, the uniqueness of the zygote lies in being the cell initiating the tight and prec ...
Cells Producing - The Journal of Immunology
... the Thy1a (Thy1.1) allele and can be recognized easily by flow cytometric analysis using a Thy1.1-specific Ab. Naive Tconv and Treg populations used in this study were confirmed by assessing Foxp3 expression (data not shown). Culture of Tconvs with antiCD3 mAb and APCs did not induce IL-17–producing ...
... the Thy1a (Thy1.1) allele and can be recognized easily by flow cytometric analysis using a Thy1.1-specific Ab. Naive Tconv and Treg populations used in this study were confirmed by assessing Foxp3 expression (data not shown). Culture of Tconvs with antiCD3 mAb and APCs did not induce IL-17–producing ...
Therapeutic approaches to Lysosomal Storage Disorders
... membranes and endosomes via the TGN. In addition endosomes allow for them to be recycled back to plasma membrane or sent to lysosomes. All molecular trafficking is regulated by a specific molecular machinery capable of recognizing and directing the molecules to their final destinations [10]. Lysosom ...
... membranes and endosomes via the TGN. In addition endosomes allow for them to be recycled back to plasma membrane or sent to lysosomes. All molecular trafficking is regulated by a specific molecular machinery capable of recognizing and directing the molecules to their final destinations [10]. Lysosom ...
Targets hidden by fibrin networks still need to find their place in the
... The treatment of rheumatoid arthritis has undergone a huge revolution in the past two decades with the incoming of biological therapies, which have been designed out of a better understanding of pathogenic mechanisms. However, while these compounds successfully prevent -or at least slow down- joint ...
... The treatment of rheumatoid arthritis has undergone a huge revolution in the past two decades with the incoming of biological therapies, which have been designed out of a better understanding of pathogenic mechanisms. However, while these compounds successfully prevent -or at least slow down- joint ...
By I\ 0. BOWER, M.A., Camb., Assistant to the Professor of Botany in
... witschia corresponds to that of Ephedra (cf. Van Tieghem, La Racine, p. 211). Meanwhile a further development has been going on in the parenchyma which surrounds the vascular tissues: this results in the formation («) of a number of irregularly arranged spicular cells (sp. c.)} whose axes are parall ...
... witschia corresponds to that of Ephedra (cf. Van Tieghem, La Racine, p. 211). Meanwhile a further development has been going on in the parenchyma which surrounds the vascular tissues: this results in the formation («) of a number of irregularly arranged spicular cells (sp. c.)} whose axes are parall ...
Gram`s staining - Micro-Rao
... Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining of these cells. In cultures of Bacillus, and Clostridium a decrease in peptidoglycan thickness during cell growth may cause some of them to appear Gram negative. Certain gro ...
... Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining of these cells. In cultures of Bacillus, and Clostridium a decrease in peptidoglycan thickness during cell growth may cause some of them to appear Gram negative. Certain gro ...
1 The LF1 Gene of Chlamydomonas reinhardtii Encodes
... was incubated with an antibody to OEE1, a chloroplast protein, (kindly provided by Bridgette Barry, University of Minnesota, St. Paul, MN) (MAYFIELD et al. 1987) at a 1:400 dilution. Immunofluorescence was performed using the method of SANDERS and SALISBURY (1995) with cells fixed in either 4% para ...
... was incubated with an antibody to OEE1, a chloroplast protein, (kindly provided by Bridgette Barry, University of Minnesota, St. Paul, MN) (MAYFIELD et al. 1987) at a 1:400 dilution. Immunofluorescence was performed using the method of SANDERS and SALISBURY (1995) with cells fixed in either 4% para ...
Centrosome misorientation mediates slowing of the cell cycle under
... Revised: Jan 30, 2012 Accepted: Feb 17, 2012 ...
... Revised: Jan 30, 2012 Accepted: Feb 17, 2012 ...
Laser Microdissection and Spatiotemporal Pinoresinol
... SDG and SDG macromolecules in flaxseed, has been proposed: a dirigent protein-assisted coupling of two E-coniferyl alcohol (1 in Figure 1A) units results in one molecule of (−)-pinoresinol (2 in Figure 1A). (−)-Pinoresinol is successively converted into (−)lariciresinol and (+)-secoisolariciresinol ...
... SDG and SDG macromolecules in flaxseed, has been proposed: a dirigent protein-assisted coupling of two E-coniferyl alcohol (1 in Figure 1A) units results in one molecule of (−)-pinoresinol (2 in Figure 1A). (−)-Pinoresinol is successively converted into (−)lariciresinol and (+)-secoisolariciresinol ...
KLF4 N-Terminal Variance Modulates Induced Reprogramming to
... OSKM cultures showed a majority of GFP+ colonies with silenced mCherry. FACS on d18 confirmed the proportions of reporter-positive cells (Figure 1C, right). Both the Total GFP+ and SSEA-1+ GFP+ fractions on d18 were consistently higher for OSKM reprogramming (mean 15.6% and 39.5%, respectively, vers ...
... OSKM cultures showed a majority of GFP+ colonies with silenced mCherry. FACS on d18 confirmed the proportions of reporter-positive cells (Figure 1C, right). Both the Total GFP+ and SSEA-1+ GFP+ fractions on d18 were consistently higher for OSKM reprogramming (mean 15.6% and 39.5%, respectively, vers ...
Novel Regulators of Vascular Development in Arabidopsis
... As part of their adaptation to various environmental conditions, vascular plants have developed a specialized tissue system, the vascular system, which provides mechanical support and functions as a delivery system for various resources, such as water, mineral nutrients, sugars and amino acids, thro ...
... As part of their adaptation to various environmental conditions, vascular plants have developed a specialized tissue system, the vascular system, which provides mechanical support and functions as a delivery system for various resources, such as water, mineral nutrients, sugars and amino acids, thro ...
Naive CD8 T cells differentiate into protective memory
... lymphopenia-induced HP. Proliferation was delayed in MHC class Ia–deficient hosts, confirming that HP of naive CD8 T cells is MHC class I–dependent (22–24). In contrast, IL-2 signals provided by injected IL-2–anti–IL-2 complexes stimulated extensive division of naive OT-I cells in the MHC class Ia– ...
... lymphopenia-induced HP. Proliferation was delayed in MHC class Ia–deficient hosts, confirming that HP of naive CD8 T cells is MHC class I–dependent (22–24). In contrast, IL-2 signals provided by injected IL-2–anti–IL-2 complexes stimulated extensive division of naive OT-I cells in the MHC class Ia– ...
Salinity Effects on the Activity of Plasma Membrane H+ and Ca2+
... Shabala and NewmanÐSalinity and Cell Wall Exchange maintained by the activity of the electrogenic H pump, H extrusion may be diminished in protoplasts following the isolation procedure. If this is the case, NaCl-induced activation of H -extrusion also may be diminished in protoplasts (Fig. 1A ...
... Shabala and NewmanÐSalinity and Cell Wall Exchange maintained by the activity of the electrogenic H pump, H extrusion may be diminished in protoplasts following the isolation procedure. If this is the case, NaCl-induced activation of H -extrusion also may be diminished in protoplasts (Fig. 1A ...
Regeneration in the aging peripheral nervous system The Harvard
... increasingly difficult as a greater percentage of the population is of advanced age. Indeed, neurodegenerative disorders represent major healthcare burdens, ...
... increasingly difficult as a greater percentage of the population is of advanced age. Indeed, neurodegenerative disorders represent major healthcare burdens, ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.