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... sometimes, but not always, adjacent to the XTC cells. The external surface of the animal pole region invariably developed as epidermis. In control experiments, also carried out at stage 7-5-8, muscle ...
... sometimes, but not always, adjacent to the XTC cells. The external surface of the animal pole region invariably developed as epidermis. In control experiments, also carried out at stage 7-5-8, muscle ...
mRNA delivery for
... Background: Generating autologous pluripotent stem cells for therapeutic applications will require the development of efficient DNA-free reprogramming techniques. Transfecting cells with in vitro-transcribed, protein-encoding RNA is a straightforward method of directly expressing high levels of repr ...
... Background: Generating autologous pluripotent stem cells for therapeutic applications will require the development of efficient DNA-free reprogramming techniques. Transfecting cells with in vitro-transcribed, protein-encoding RNA is a straightforward method of directly expressing high levels of repr ...
changes in the ultrastructure of capsicum annuum l. seedlings roots
... more numerous, vacuoles containing electron-opaque formations (Figs 7, 8), similar to those described in the vacuoles of the cap. The lobate cell nuclei had irregular, folded contours with deep invaginations (Fig. 8), and at some place a partial degradation of the nucleus membrane occurred (Fig. 9). ...
... more numerous, vacuoles containing electron-opaque formations (Figs 7, 8), similar to those described in the vacuoles of the cap. The lobate cell nuclei had irregular, folded contours with deep invaginations (Fig. 8), and at some place a partial degradation of the nucleus membrane occurred (Fig. 9). ...
Interferon- and interferon- differentially affect pancreatic
... ern blot analysis. Laser densitometric analysis indicated that IFN-g treatment increased H-2K a-chain and b2m mRNA expression by bTC6-F7 cells 5-fold and 10-fold compared with untreated controls (3.3 ADU without IFN-g vs. 16.3 ADU with IFN-g for H-2K a-chain; 1.4 ADU without IFN-g vs. 14 ADU with IF ...
... ern blot analysis. Laser densitometric analysis indicated that IFN-g treatment increased H-2K a-chain and b2m mRNA expression by bTC6-F7 cells 5-fold and 10-fold compared with untreated controls (3.3 ADU without IFN-g vs. 16.3 ADU with IFN-g for H-2K a-chain; 1.4 ADU without IFN-g vs. 14 ADU with IF ...
ELECTRON-MICROSCOPE ILLUSTRATIONS OF DIVISION IN
... cells, the original electron-dense cell-wall layer is present (figs. 11c and 16). Later this wall disappears and the daughter cells have rounded ends and the electron-transparent layer between them is continuous with the electrontransparent layer of the cells themselves (figs 1Id and 17). These last ...
... cells, the original electron-dense cell-wall layer is present (figs. 11c and 16). Later this wall disappears and the daughter cells have rounded ends and the electron-transparent layer between them is continuous with the electrontransparent layer of the cells themselves (figs 1Id and 17). These last ...
the single-cell preparation guide
... single-cell suspensions. Whether you are working with cultured or primary cells, adherent cells, or cells in suspension, the condition of the cells is a critical factor in ensuring high cell capture efficiency and optimal chemistry performance. In this guide, we provide optimized protocols for disso ...
... single-cell suspensions. Whether you are working with cultured or primary cells, adherent cells, or cells in suspension, the condition of the cells is a critical factor in ensuring high cell capture efficiency and optimal chemistry performance. In this guide, we provide optimized protocols for disso ...
Concept 1.1 Introduction to the Sciences Lesson Essential Question
... Lesson Essential Question(s): How do different types of cells develop from a single stem cell? How are cells organized to carry out the specialized functions of an organism? What are the five levels of organization of the human body? Vocabulary: Determination, Differentiation, Organ, Organ system, O ...
... Lesson Essential Question(s): How do different types of cells develop from a single stem cell? How are cells organized to carry out the specialized functions of an organism? What are the five levels of organization of the human body? Vocabulary: Determination, Differentiation, Organ, Organ system, O ...
Megakaryoblastic Leukemia-1/2, a
... of the tissue specificity, however, myocardin was not a candidate for regulation of skeletal muscle-specific genes or growth factor-inducible immediate early genes. We recently identified megakaryoblastic leukemia-1 (MKL1), a protein with a similarity to myocardin, as a potent transcriptional activa ...
... of the tissue specificity, however, myocardin was not a candidate for regulation of skeletal muscle-specific genes or growth factor-inducible immediate early genes. We recently identified megakaryoblastic leukemia-1 (MKL1), a protein with a similarity to myocardin, as a potent transcriptional activa ...
BioVision
... Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. ...
... Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. ...
Effects of dietary components on Tight junctions (TJ) Lauric acid
... activation of the MAPK, ERK, and JNK (91); thus, glutamine could potentially modulate TJ via a MAPK-dependent signal transduction pathway. As well as having nutritional value, trace elements such as zinc may also assist with the maintenance of intestinal barrier integrity. Caco-2 cells grown in zin ...
... activation of the MAPK, ERK, and JNK (91); thus, glutamine could potentially modulate TJ via a MAPK-dependent signal transduction pathway. As well as having nutritional value, trace elements such as zinc may also assist with the maintenance of intestinal barrier integrity. Caco-2 cells grown in zin ...
RNA Interference of Signal Peptide-binding Protein SRP54 Elicits
... (5). SRP then targets the nascent chain-ribosome complex to the ER membrane by interacting with a membrane-bound SRP receptor. After this docking event that is GTP-dependent, the translocation of the polypeptide takes place co-translationally through the protein pore, the translocon (6, 7). The well ...
... (5). SRP then targets the nascent chain-ribosome complex to the ER membrane by interacting with a membrane-bound SRP receptor. After this docking event that is GTP-dependent, the translocation of the polypeptide takes place co-translationally through the protein pore, the translocon (6, 7). The well ...
"Lymphocyte Activation Signals: Transduction".
... becomes highly phosphorylated after TCR-antigen recognition. LAT then serves as a docking site for numerous other molecules that are recruited based on interactions of SH2 domains with phosphorylated tyrosine. BLNK serves a function similar to LAT, but it is expressed in B cells. BLNK is located in ...
... becomes highly phosphorylated after TCR-antigen recognition. LAT then serves as a docking site for numerous other molecules that are recruited based on interactions of SH2 domains with phosphorylated tyrosine. BLNK serves a function similar to LAT, but it is expressed in B cells. BLNK is located in ...
N-glycosylation and microtubule integrity are involved in apical
... better-known marker, prostate-specific antigen (4). In contrast to other prostate-specific proteins, such as prostate-specific antigen or prostatic acid phosphatase, PSMA is a transmembrane protein expressed on the surface of the prostatic epithelium. As an integral membrane protein, PSMA offers a p ...
... better-known marker, prostate-specific antigen (4). In contrast to other prostate-specific proteins, such as prostate-specific antigen or prostatic acid phosphatase, PSMA is a transmembrane protein expressed on the surface of the prostatic epithelium. As an integral membrane protein, PSMA offers a p ...
Human Monocytes Arachidonic Acid Reacylation Reactions in
... the AA has been incorporated into PLs, a remodeling process carried out by CoA-independent transacylase transfers AA from choline glycerophospholipids (PCs) to ethanolamine glycerophospholipids, in a process that takes several hours to take place in primary cells but is strikingly rapid in tumor cel ...
... the AA has been incorporated into PLs, a remodeling process carried out by CoA-independent transacylase transfers AA from choline glycerophospholipids (PCs) to ethanolamine glycerophospholipids, in a process that takes several hours to take place in primary cells but is strikingly rapid in tumor cel ...
document
... voltages exist across the membranes of excitable cells when they are at rest; these voltages are called resting membrane potentials and they exist because of the unequal distribution of Na+, K+, Cl- and protein on either side of the plasma membrane ...
... voltages exist across the membranes of excitable cells when they are at rest; these voltages are called resting membrane potentials and they exist because of the unequal distribution of Na+, K+, Cl- and protein on either side of the plasma membrane ...
B. - Genetics
... environmental conditions and acquisition of novel phenotypic traits. Several mechanisms exist to ensure stability and to prevent variation: recombination and DNA repair prevent mutation; the effects of mutation on protein structure are minimized by codon redundancy; gene expression and enzyme activi ...
... environmental conditions and acquisition of novel phenotypic traits. Several mechanisms exist to ensure stability and to prevent variation: recombination and DNA repair prevent mutation; the effects of mutation on protein structure are minimized by codon redundancy; gene expression and enzyme activi ...
CHAPTER 24 The Immune System and Disease
... B and T cells actually recognize and respond to antigens on pathogens. Antigens are molecules that the immune system recognizes as foreign to the body. Antigens are also found on cancer cells and the cells of transplanted organs. They trigger the immune system to react against the cells that carry t ...
... B and T cells actually recognize and respond to antigens on pathogens. Antigens are molecules that the immune system recognizes as foreign to the body. Antigens are also found on cancer cells and the cells of transplanted organs. They trigger the immune system to react against the cells that carry t ...
What is cellular Senescence? - Roswell Park Cancer Institute
... and mir-146b), which tune down the expression IL-6, IL-8, and possibly other SASP proteins, presumably to prevent the SASP from generating a persistent acute inflammatory response. Despite this dampening effect, the SASP can nonetheless continue to generate low-level chronic inflammation. The accumu ...
... and mir-146b), which tune down the expression IL-6, IL-8, and possibly other SASP proteins, presumably to prevent the SASP from generating a persistent acute inflammatory response. Despite this dampening effect, the SASP can nonetheless continue to generate low-level chronic inflammation. The accumu ...
Perifosine, an oral bioactive novel alkylphospholipid
... Xenograft murine model Beige-nude-xid (BNX) mice (5 to 6 weeks old) were obtained from Frederick Cancer Research and Development Center (Frederick, MD). All animal studies were conducted according to protocols approved by the Animal Ethics Committee of the Dana-Farber Cancer Institute. The mice were ...
... Xenograft murine model Beige-nude-xid (BNX) mice (5 to 6 weeks old) were obtained from Frederick Cancer Research and Development Center (Frederick, MD). All animal studies were conducted according to protocols approved by the Animal Ethics Committee of the Dana-Farber Cancer Institute. The mice were ...
The Role of Exopolysaccharides in Adhesion of
... Using chemically clean glass coverslips as the test surface, the association between adsorbed carbohydrate material and attached cells was assayed at regular intervals (Fig. 1). With both strains, carbohydrate material accumulated gradually over the initial 5-6 h exposure, then increased rapidly ove ...
... Using chemically clean glass coverslips as the test surface, the association between adsorbed carbohydrate material and attached cells was assayed at regular intervals (Fig. 1). With both strains, carbohydrate material accumulated gradually over the initial 5-6 h exposure, then increased rapidly ove ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.