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Counting Chloroplasts
Counting Chloroplasts

... Using the forceps, place a section of the plant you are to study into the petri dish of water. Use the forceps to carefully remove one small, thin leaf from the plant and place it on a clean glass slide. Make a wet mount of the leaf. Be careful not to include air bubbles under the cover slip. See Sk ...
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... of DNA strand breaks in early S-phase. Calu-6 cells were treated with 0.4% DMSO or 100 nM LY2606368 for seven hours. The plates were fixed and stained for DNA, pH2AX and DNA strand breaks by TUNEL assay (see Materials and Methods). Analysis was by high content imaging (Thermo Scientific Cell Insight ...
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Cellular differentiation



In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.
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