video slide - Issaquah Connect
... in each pellet, establishing a baseline for further experiments. (and chloroplasts if cells In the next series of experiments, researchers used are from a Pellet rich in biochemical methods to determine the metabolic functions plant) “microsomes” (pieces of associated with each type of organelle. Re ...
... in each pellet, establishing a baseline for further experiments. (and chloroplasts if cells In the next series of experiments, researchers used are from a Pellet rich in biochemical methods to determine the metabolic functions plant) “microsomes” (pieces of associated with each type of organelle. Re ...
Detergent-resistant membranes and the protein
... Genome Biology 2003, 4:234 The electronic version of this article is the complete one and can be ...
... Genome Biology 2003, 4:234 The electronic version of this article is the complete one and can be ...
ARVO 2017 Annual Meeting Abstracts 231 RPE metabolism
... Results: Fundus and infrared reflectance revealed areas of retinal atrophy and subretinal debris. OCT demonstrated disruption of the RPE and ellipsoid layers. Pinpoint autofluorescence was evident in affected areas. ERG exhibited a decreased scotopic response in both the A and B-waves. Histopatholog ...
... Results: Fundus and infrared reflectance revealed areas of retinal atrophy and subretinal debris. OCT demonstrated disruption of the RPE and ellipsoid layers. Pinpoint autofluorescence was evident in affected areas. ERG exhibited a decreased scotopic response in both the A and B-waves. Histopatholog ...
Show and tell: cell biology of pathogen invasion
... protein interactions in vivo. This FRET-related method relies on measurements of the reduction in the time that the donor fluorescent molecule remains in the excited state in the presence of an acceptor molecule as a measure of protein–protein interaction [5,6,23,24]. Other variations on FRET, suc ...
... protein interactions in vivo. This FRET-related method relies on measurements of the reduction in the time that the donor fluorescent molecule remains in the excited state in the presence of an acceptor molecule as a measure of protein–protein interaction [5,6,23,24]. Other variations on FRET, suc ...
Isolation of N-Acetylmuramoyl-L-Alanine Amidase Gene (amiB) from
... survive during passage through the stomach. They finally attach and colonize within the intestine of the fish. In V. cholerae, the expression level of heat-shock proteins changes in order to maintain the homeostasis of cytoplasmic pH; about 110 proteins of V. cholerae have been shown to be affected ...
... survive during passage through the stomach. They finally attach and colonize within the intestine of the fish. In V. cholerae, the expression level of heat-shock proteins changes in order to maintain the homeostasis of cytoplasmic pH; about 110 proteins of V. cholerae have been shown to be affected ...
Tomato LeAGP-1 is a plasma membrane-bound
... with 1% (w/w) Triton X-100 overnight at 4 C. The detergent-treated PM vesicles were centrifuged for 1 h at 100 000 g at 4 C. Supernatants were incubated with (b-D-galactosyl)3 Yariv reagent to precipitate and purify AGPs as previously described (Gao et al. 1999). Alternatively, absolute ethanol wa ...
... with 1% (w/w) Triton X-100 overnight at 4 C. The detergent-treated PM vesicles were centrifuged for 1 h at 100 000 g at 4 C. Supernatants were incubated with (b-D-galactosyl)3 Yariv reagent to precipitate and purify AGPs as previously described (Gao et al. 1999). Alternatively, absolute ethanol wa ...
Cell Culture
... The ratio of the number of bronchial epithelial cells with positive results for survivin in the nucleus or cytoplasm to the total number of bronchial epithelial cells was determined in blind fashion for six fields (three from the upper lobe and three from the lower lobe) for all groups of mice (n = ...
... The ratio of the number of bronchial epithelial cells with positive results for survivin in the nucleus or cytoplasm to the total number of bronchial epithelial cells was determined in blind fashion for six fields (three from the upper lobe and three from the lower lobe) for all groups of mice (n = ...
Evolutionary aspects of non-cell-autonomous regulation in vascular
... In seedless plants, the knowledge on cell wall composition, lipidomics and hormonal regulatory networks is limited compared to angiosperms. However, genome sequencing in the moss Physcomitrella patens and the spike moss Selaginella moellendorfii has recently provided a solid basis for future analyse ...
... In seedless plants, the knowledge on cell wall composition, lipidomics and hormonal regulatory networks is limited compared to angiosperms. However, genome sequencing in the moss Physcomitrella patens and the spike moss Selaginella moellendorfii has recently provided a solid basis for future analyse ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
... where it interacts with and enhances the formation of F-actin10,11. Rac1 and other Rho family GTPases have a crucial role in regulating actin dynamics that underlie formation of lamellipodia, filopodia, and actin stress fibers. The activation of these G-proteins is tightly regulated by a variety of ...
... where it interacts with and enhances the formation of F-actin10,11. Rac1 and other Rho family GTPases have a crucial role in regulating actin dynamics that underlie formation of lamellipodia, filopodia, and actin stress fibers. The activation of these G-proteins is tightly regulated by a variety of ...
On line Supplement : AND SACS DURING BRONCHOSCOPY.
... is made of four components : a miniaturized flexible fibered probe, a Laser Scanning Unit, a spectroscopic channel, and a dedicated control and acquisition software that allows the reconstruction of dynamic images in real-time. The fiberoptic miniprobe (Alveoflex Confocal Miniprobe®, Mauna Kea Techn ...
... is made of four components : a miniaturized flexible fibered probe, a Laser Scanning Unit, a spectroscopic channel, and a dedicated control and acquisition software that allows the reconstruction of dynamic images in real-time. The fiberoptic miniprobe (Alveoflex Confocal Miniprobe®, Mauna Kea Techn ...
Chapter 4 Powerpoint
... fibers that organizes structures and activities in the cell The cytoskeleton is a network of fibers extending throughout the cytoplasm ...
... fibers that organizes structures and activities in the cell The cytoskeleton is a network of fibers extending throughout the cytoplasm ...
A: DNA
... Inorganic molecules, water, ions (How is this organization achieved? Expenditure of energy!) Lectures 3 & 4 & 5 ...
... Inorganic molecules, water, ions (How is this organization achieved? Expenditure of energy!) Lectures 3 & 4 & 5 ...
Herpesvirus Saimiri-induced Proteins in Lyrically Infected Cells. I
... was no synchronous synthesis of virus proteins during this r a t h e r long t i m e i n t e r v a l and the synthesis of host cell proteins ceased late and rather incompletely. Pulse-labelling w i t h [35S]methionine during the course o f virus infection allowed only the d e t e c t i o n o f the la ...
... was no synchronous synthesis of virus proteins during this r a t h e r long t i m e i n t e r v a l and the synthesis of host cell proteins ceased late and rather incompletely. Pulse-labelling w i t h [35S]methionine during the course o f virus infection allowed only the d e t e c t i o n o f the la ...
Survival Mechanisms of Extremophiles
... (Konings et al. 2002). Shimada et al. (2002) provided concrete evidence of this phenomenon in Thermoplasma acidophilum, whose cell membranes are made of tetraether lipids. Other examples of acidophiles include Picrophilus oshimae (van de Vossenberg et al. 1998a), Sulfolobus solfataricus (van de Voss ...
... (Konings et al. 2002). Shimada et al. (2002) provided concrete evidence of this phenomenon in Thermoplasma acidophilum, whose cell membranes are made of tetraether lipids. Other examples of acidophiles include Picrophilus oshimae (van de Vossenberg et al. 1998a), Sulfolobus solfataricus (van de Voss ...
1. INTRODUCTION The skin is the largest organ in the human body
... extend into deeper tissues, causing brown or blackened skin that may be numb (Ho-Asjoe et al 1999). ...
... extend into deeper tissues, causing brown or blackened skin that may be numb (Ho-Asjoe et al 1999). ...
P19 embryonal carcinoma cells
... with P19 cells indicated that they differentiated very inefficiently under these conditions. However, differentiation of P19 cells can be induced if aggregates are exposed to non-toxic concentrations of a number of drugs. The drugs most effective in inducing differentiation of P19 cells are retinoic ...
... with P19 cells indicated that they differentiated very inefficiently under these conditions. However, differentiation of P19 cells can be induced if aggregates are exposed to non-toxic concentrations of a number of drugs. The drugs most effective in inducing differentiation of P19 cells are retinoic ...
BCL-2 Expression and Mitochondrial Activity in
... complexes with death-accelerating homologs7 and functions in antioxidant pathways in preventing apoptosis.8The precise biochemical activities of bcl-2 protein and its homologs are not known, however. Because of its localization to mitochondrial membranes,' protection by BCL-2 has been previously ass ...
... complexes with death-accelerating homologs7 and functions in antioxidant pathways in preventing apoptosis.8The precise biochemical activities of bcl-2 protein and its homologs are not known, however. Because of its localization to mitochondrial membranes,' protection by BCL-2 has been previously ass ...
Mutations in a- and/ -Tubulin Affect Spindle Chinese Hamster Ovary
... 10004) is a subclone of the CHO cell line Pro-5 (29). The ~-tubulin mutants Cmd-4 (10193) and Grs-2 (10132) and the a-tubulin mutant Tax-1 00576) were derived from the wild-type strain after ethylmethane sulfonate mutagenesis (10193, 10132) or UV-irmdiation (10576) and were selected on the basis of ...
... 10004) is a subclone of the CHO cell line Pro-5 (29). The ~-tubulin mutants Cmd-4 (10193) and Grs-2 (10132) and the a-tubulin mutant Tax-1 00576) were derived from the wild-type strain after ethylmethane sulfonate mutagenesis (10193, 10132) or UV-irmdiation (10576) and were selected on the basis of ...
Intracellular accumulations 2014-2015Dr.Ban Jumaa Intracellular
... The deposition of calcium salts in normal tissues, and it almost always results from hypercalcemia secondary to disturbance in calcium metabolism. Metastatic calcification can occur widely throughout the body but principally affects the interstitial tissues of the vasculature, kidneys, lungs, and ga ...
... The deposition of calcium salts in normal tissues, and it almost always results from hypercalcemia secondary to disturbance in calcium metabolism. Metastatic calcification can occur widely throughout the body but principally affects the interstitial tissues of the vasculature, kidneys, lungs, and ga ...
The Assimilation of Amino-acids by Bacteria
... If the action of the tyrocidin is to liberate amino-acids from the internal environment of the streptococcal cells, this would be shown by evolution of CO,, as the lysine or glutamic acid is attacked by the decarboxylase prepara; tion. Fig. 1 shows the results obtained in such an experiment. The add ...
... If the action of the tyrocidin is to liberate amino-acids from the internal environment of the streptococcal cells, this would be shown by evolution of CO,, as the lysine or glutamic acid is attacked by the decarboxylase prepara; tion. Fig. 1 shows the results obtained in such an experiment. The add ...
Nice, T.J., W. Deng, L. Coscoy and D.H. Raulet. 2010. Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase. J Immunol 185:5369-5376.
... and nine known human ligands (RAET1a–RAET1g [also known as UL-16 binding proteins], MICA, and MICB) that are all distantly related to MHC class I. However, because of their relatively rapid rate of evolution, it has not been possible to draw direct lines of homology between individual mouse and huma ...
... and nine known human ligands (RAET1a–RAET1g [also known as UL-16 binding proteins], MICA, and MICB) that are all distantly related to MHC class I. However, because of their relatively rapid rate of evolution, it has not been possible to draw direct lines of homology between individual mouse and huma ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).