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BACTERIAL GENETICS
BACTERIAL GENETICS

... Once the DNA is transferred from the donor to the recipient cell it can integrate into the host cell chromosome by recombination. 1) Homologous recombination: in which two pieces of DNA that has extensive homologous regions pair up and exchange pieces by the process of breakage and reunion 2) Non ho ...
NOTE: The provided figures may be useful and beneficial
NOTE: The provided figures may be useful and beneficial

... NOTE: The provided figures may be useful and beneficial & you should consider including them in your learning log. Keep up the good work & be an Intentional Learner. Chapter 16 1. In Griffith’s experiment, why was he able to rule out the possibility that the R cells could have simply used the capsul ...
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... 96 well dishes (1 x 104 cells per well). Full length recombinant Tat protein (positive control; ...
The Human Genome Project
The Human Genome Project

... The Human Genome Project What is the Human Genome Project? • U.S. govt. project coordinated by the Department of Energy and the National Institutes of Health • goals (1998-2003) – identify the approximate 100,000 genes in human DNA – determine the sequences of the 3 billion bases that make up human ...
Mouse Genetics
Mouse Genetics

Biotechnology and Mutation Quiz key
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... sequences of DNA. In 1990, an international effort began to analyze the human DNA sequence. What is the name given to this global research that was completed in 2003? A. The DNA Revolution B. The National Institute of Health C. The Geneva Conference D. The Human Genome Project ...
13-2 Manipulating DNA
13-2 Manipulating DNA

...  Knowing the sequence of an organism’s DNA allows researchers to study specific genes, to compare them with the genes of other organisms, and to try to discover the functions of different genes and gene combinations. ...
GE & Profiling iQuiz
GE & Profiling iQuiz

... When the DNA from individuals is analysed a unique DNA profile is made and a result similar to a bar code is obtained. This DNA profile formation is also known as genetic ...
Life 101 - findyourtao2011
Life 101 - findyourtao2011

... Definition: A change in the DNA. Remember, DNA is the code of life. It determines what traits will be expressed and what traits won’t be expressed. A single change in the sequence of DNA is a mutation. A mutation can be neutral, positive or negative for an organism. A negative mutation means it is h ...
VIRAL VECTORS IN GENE THERAPY
VIRAL VECTORS IN GENE THERAPY

... transgene of interest and expressed on plasmids in the packaging cell line. • Because the non-essential genes lack the packaging sequence, they are not included in the virion particle. ...
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... or at non-essential amino acid positions. This method is good for fine-scale mutagenesis. b) homologous recombination - works in bacteria, yeast, mice and other mammals. It does not work well in Drosophila, although a complex experimental approach has been developed. This method has been used to kno ...
fsu neuroscience program - Florida State University
fsu neuroscience program - Florida State University

... intra-cellular signals that allow neurotrophins (nerve growth factors) to keep neurons alive and growing and to manipulate these signals with the hope that this will bring insights into potential therapies for the diseased or traumatized nervous system. Dr. Miller and her team have also recently iso ...
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Ch.5

... 2)Multiple alleles-a gene that exists in more than 2 allele forms, although a diploid individual only has 1 or 2 of them. • Although each person has 2 alleles for any autosomal gene (one on each chromosome) a gene can exist in more than 2 allelic forms • Ex: Blood Types ...
Ch 11- Controlling Gene Expression
Ch 11- Controlling Gene Expression

... – Activators are one type that bind to enhancer DNA sequences; sequences that regulate far from gene – DNA bends and TF’s bind to create an area where RNA pol can bind to – Silencers- are sequences that repressors bind to; stop transcription initiation • Coordinating gene expression- eukaryotes rare ...
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A Closer Look at Conception
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... • Artificial Insemination: Doctors inject sperm into the woman’s uterus. The sperm could be from her partner OR a donor. • In vitro fertilization: Doctors fertilize the woman’s ovum with the man’s sperm in the lab. If fertilization occurs, the zygote is placed in the woman’s uterus ...
Slides-Brian_Charlesworth-Sex_and_molecular_evolution
Slides-Brian_Charlesworth-Sex_and_molecular_evolution

... Let the ratio of the fitnesses of A2 and A1 individuals be 1 – s . The quantity s measures the intensity of selection, and is called the selection coefficient. If q is the frequency of A2, the change in q is q, given approximately by sq(1 – q). ...
Biol 505 EXAM 1 (100 points): Due Wed 10/14/09 at the beginning
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... Biol 505 EXAM 1 (100 points): Due Wed 10/14/09 at the beginning of class. Hard copy only. Although this is an open book/open note exam, you are taking this exam under the honor system, which requires that you refrain from sharing any information regarding the exam with any of your classmates. The ho ...
BIOTECHNOLOGY AND GENETIC ENGINEERING
BIOTECHNOLOGY AND GENETIC ENGINEERING

... that has demonstrated the ability to turn adult stem cells into embryo-like stem cells. These are called “induced pluripotent stem cells” (IPSCs). ...
76d26f86fc8fd4690d9502156978f6866d36b66a
76d26f86fc8fd4690d9502156978f6866d36b66a

... Recombinant DNA Technology A. Genetic Engineering - faster, more reliable method for_______________ the frequency of a specific allele in a population. B. Recombinant DNA is made by___________ or_____________ fragments of DNA from different ________________ C. Transgenic organisms contain __________ ...
Genetic modification: an overview for non
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... common to all life, it is also possible to produce synthetic genes. This technology is called genetic modification or genetic engineering. There are three major differences between selective breeding and genetic modification: ...
BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 2 Ch 4, 11
BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 2 Ch 4, 11

Reporting Category 2
Reporting Category 2

... Mutations can create new traits Not always BAD; some are beneficial  think about mutations that lead to adaptations! Passed on through SEX CELLS (gametes) Caused by a variety of things such as ...
TOC  - G3: Genes | Genomes | Genetics
TOC - G3: Genes | Genomes | Genetics

... Genome editing is a powerful method to study gene function. In this work, Vilain and Vanhauwaert et al. present a novel genome editing methodology for fruit flies based on MiMIC transposons that are present throughout the genome. The methodology enables the engineering of almost every gene in the gen ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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