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glossary of technical terms
glossary of technical terms

... kinds of bases, which are abbreviated A, C, T and G. A DNA fragment that is ten bases long might have a base sequence of, for example, ATCGTTCCTG. The particular sequence of bases encodes important information in an individual’s genetic blueprint and is unique for each individual (except identical t ...
Document
Document

... Human – mouse hybrids are usually made using established mouse cell culture lines and human fibrocytes or leukocytes.  Important features of human-mouse hybrids:1. Mouse-human chromosomes are easily distinguished. 2.Both sets of chromosomes of human and mouse are expressed in hybrid cells. ...
DNA, Chromosomes & Genes - Blountstown Middle School
DNA, Chromosomes & Genes - Blountstown Middle School

... – Sequences carry the information needed for constructing proteins • Proteins provide the structural components of cells and tissues as well as enzymes for essential biochemical reactions. ...
Chapter 13 Genetic Engineering
Chapter 13 Genetic Engineering

... Restriction enzymes: are special enzymes that cut DNA in specific locations by recognizing certain base sequences ...small pieces are easier to work with, study and identify now, let's organize: Gel electrophoresis: separates DNA fragments through a gel using electricity ...
siRNA expression vector pRNAT-H1
siRNA expression vector pRNAT-H1

... Technology and can be used to move DNA sequence (any genes) into multiple vector systems for functional analysis and protein expression. * Limited Use Label License: The use of CMV promoter is covered under U. S. Patent No. 5,168,062 and 5,385,839 owned and licensed by the University of Iowa Researc ...
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Thalassaemia

... antibiotic resistance Main circular DNA ...
Mutations
Mutations

... genes, which means genes can be Removed, put together, and recombined: 1. Cut out the desired DNA of the gene 2. Combine that DNA with that of the recipient 3. Insert it into the new organism ...
Infection cycle: DNA viruses
Infection cycle: DNA viruses

... • Monogenic proteins with individual promotors • Uses host RNA-P • Multliple splicing of ...
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... the genotype________________. We show it by using __CAPITOL__ letters. Recessive gene – The trait that will show up only when _it is the only allele present (no dominant allele to “take over). We show it by using _lowercase_letters. Answer: Where do an organism’s traits come from? Directly from _par ...
Plant Transformation
Plant Transformation

... • nucleic acid sequences encoding easily assayed proteins • Reporter genes include -galactosidase (encoded by lacZ), -glucuronidase (encoded by uidA), chloramphenicol acetyltransferase, luciferase and green fluorescent protein (GFP) . ...
Genetics of prokaryotic organisms
Genetics of prokaryotic organisms

... protists of phylum Apicomplexa (Pasmodium,Toxoplasmosma……) ...
userfiles/1290/Genetics Review Sheet - Answer Key
userfiles/1290/Genetics Review Sheet - Answer Key

... the genotype________________. We show it by using __CAPITAL__ letters. Recessive gene – The trait that will show up only when _it is the only allele present (no dominant allele to “take over). We show it by using _lowercase_letters. Answer: Where do an organism’s traits come from? Directly from _par ...
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I. What is DNA Replication?

... Unit 4: Modern Genetics Aim 2: How does the cell make new DNA ...
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Gene Technology PowerPoint

... Genetic engineering  Recombinant DNA - molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological organisms. ...
22. Oncogenes
22. Oncogenes

... Tumor suppressors: Genes that in normal form prevent cancer. Encode transcription factors, proteins that control cell cycle, DNA repair enzymes. Mutation leads to recessive loss of function. 2. Discovery of oncogenes 1960's study of oncogenic retroviruses retroviruses:  RNA is genetic material  En ...
Genetics and Genetic Engineering
Genetics and Genetic Engineering

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bot 458h1f - plant molecular biology and biotechnology
bot 458h1f - plant molecular biology and biotechnology

... expression will be discussed in detail. As well, how plants sense and respond at the molecular level to environmental stresses such as drought, salinity, cold and disease, will be discussed. Recent examples from the original literature will be used to study how plant gene expression and signal trans ...
Rita Levi Montalcini was born on April 22nd, 1909
Rita Levi Montalcini was born on April 22nd, 1909

... GM crops are organisms the DNA of which has been modified using genetic engineering techniques, for many different purposes; the main one being to develop crop and animal varieties able to survive without the use of armful chemicals in extreme weather conditions. Many people don’ t agree with the GM ...
Some No-Nonsense Facts on
Some No-Nonsense Facts on

... improve plants and animals. Geneticists specific location on a chromosome selectively control traits to benefit the and determines a particular community. An example is teosinte characteristic in an organism. Teosinte has been selectively bred since Genes undergo mutation when 8000BC. Teosinte has b ...
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What is Genetic Modification?

... genetic engineering more widely accepted. Opponents fear that companies will go on to develop other genetically modified plants from which they can make a profit. A situation could develop where the large companies own the rights to all the good crops ...
Candidate Gene Approach
Candidate Gene Approach

... Fig. 4. Examples of mutations with specific defects in the development of zebrafish embryos. AI! embryos shown are 24 hours-old. (A)Wildtype. (B) cyclops mutant with partial!y fused eyes (Hatta et a/.. 1993). (C) Wildtype. (D) cyclops mutant showing the absence of a floor plate (Hatta et al., 1993) ...
in non sex cells
in non sex cells

... trait of an individual can be determined by one genes, but is usually determined by the interaction of many different genes. A single gene can influence more than one trait. A human cell contains many thousands of different genes coding for many different traits. ...
LATg Training Course - AZ Branch AALAS Homepage
LATg Training Course - AZ Branch AALAS Homepage

... • DNA is a long string (polymer) of 4 bases • These bases universal! – A = Adenosine – T = Thymine – C = Cytosine – G = Guanine • The order (sequence) of the bases is what makes one gene different from another gene. ...
ALE #7
ALE #7

投影片 1
投影片 1

... What are the inherent differences between PML-RARaand PLZF-RARaat the molecular level? • Gene expression analysis on random APLsfrom humans followed by identification of the fusion gene • Correlate gene expression in PMLRARαvs. PLZF-RARαvs. normal myeloid cells Image removed due to copyright consid ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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