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SK_DifficultProblems.
SK_DifficultProblems.

... Compositional heterogeneity Stationarity: A standard assumption of most phylogeny reconstruction methods is that underlying substitution processes are the same across the tree When violated, biases arise that provide signals in the data that can overwhelm the “true” phylogenetic signal Shifting sub ...
Overview of B-Cell Development
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... to D-J recombination producing VDJ variable region gene. ...
HANDOUT: CH 18 pt 1 Study
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... 10) Why are repressible enzymes generally associated with anabolic pathways and how is this an advantage to the ...
Section 11.2 - CPO Science
Section 11.2 - CPO Science

... to 1916) examined the nucleus of the cell of a grasshopper under a microscope. • Sutton observed cell parts separating during cell division. • Soon chromosomes were discovered to contain genes. ...
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Response from Women`s and Children`s Health Network Institutional

... method SDN-3 however should be classified as a GMO, as (1) it clearly results in additional functions to endogenous genes (e.g. gene tagging) or introduce new ectopic gene or gene regulatory elements and (2) is in line with other methods of transgene insertion (only done in different way that facili ...
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centromere

... • Unique sequence DNA (strictly speaking 1 copy, but in practice this also includes sequences with only a few copies) ...
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No Slide Title

... chromatin remodeling complex, and RNA pol II. ...
Extensions and Exceptions to Mendel*s Laws
Extensions and Exceptions to Mendel*s Laws

...  Myoclonal Epilepsy and Ragged Red Fiber Disease: only affects child of affected mother, not affected father; blotchy red patches, deafness, seizures, brain function problems ...
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... • Rather, it is bidirectional; genes affect children’s behavior and experiences, but their experiences and behavior also affect gene expression. • Stimulation of both internal and external environments (to the child) triggers gene activity. • Epigenesis means the development of the individual result ...
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...  Non-autonomous (successful freeloaders! ‘borrow’ RT from other sources such as LINEs)  ~100-300bp long  Internal polymerase III promoter  No proteins  Share 3’ ends with LINEs  3 related SINE families in humans are active Alu, inactive MIR and Ther2/MIR3. 3) Long Terminal Repeats (LTR) Repeat ...
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... Conservatively, the amount of DNA necessary to encode this protein of 445 amino acids is 445 × 3 = 1335 base pairs. When compared with the actual amount of DNA used, 60 kb, the gene appears to be roughly 45 times larger than necessary. This “extra” DNA mostly represents the introns that must be corr ...
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Angelina Jolie

... MLH1, MSH2, MSH6, and PMS2 genes are responsible for repairing mistakes that occur during the process of DNA replication, so when those genes are mutated, the proper repair of DNA replication mistakes are prevented. As these mutated cells continue to divide, the collected mistakes can lead to an unc ...
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pNZ:vig Vector information: IRES

... Suitable host strain: NZ9000 and other NICE system vectors derivatives ...
Genetic Technology
Genetic Technology

... c. Process for Making Recombinant DNA: 1. Cleave DNA  cut the desired gene (DNA sequence) using a restriction enzyme as well as the host DNA  Restriction enzymes are proteins used to cut DNA between certain neulceotides on both strands of DNA  There are many different restriction enzymes that ar ...
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... cross-references these sequences to Ensembl Core databases for each species to gather genomic feature information via stable_ids. Aequatus then processes the comparative and feature data to provide a visual representation of the phylogenetic and structural relationships among the set of chosen speci ...
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... What about human females? Does this pattern (called a mosaic) ever occur? RARELY, if a woman has the gene for Duchenne’s muscular dystrophy on one X chromosome, there will be SOME weakness in certain of her muscles where that version of the X chromosome is not active. But it’s only a mild disorder, ...
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... 3. Know the natural and the synthetic methods used to introduce DNA into a cell (transformation, transduction, etc) 4. Know what a vector is with regard to recombinant technology 5. Describe the difference between conventional and molecular diagnostics. a) name at least three conventional methods of ...
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... New evidence that integration events can sometimes activate genes that stimulate cell division (CANCER ENSUES!) Site of integration can have an effect on expression of gene (No way to control this) ...
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... • Could be in charge of making a protein (like the gene for the molecule keratin has its nucleotides in an order such that the amino acid sequence that is made from those directions will make keratin) • Could be a ‘regulatory’ gene – like a foreman in a factory who produces nothing directly, but who ...
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... Leukemia is type of cancer characterized by the uncontrolled proliferation of white blood cells (leukocytes). Chronic Myelogenous Leukemia (CML) is a type of leukemia primarily caused by a translocation that relocates an oncogene, called abl, from the long arm of chromosome 9 to the long arm of chro ...
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... • 99.9% confidence limit was estimated by testing the coefficient of variance (CV) for replicates ...
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Recombinant_Project_Proposal_revised_(really)

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Review Guide Genetics

... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
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... Nonsense – when the mutation changes an amino acid sequence into a stop codon. The process will stop too soon and the protein won’t be finished. This missing part of the protein will cause it to not function properly. (ex: you accidently cut off the last three words of the sentence so you don’t know ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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