ucsc genome research primer - Center for Biomolecular Science
... nucleotides, which are organized into DNA molecules—the double helix. The nucleotides, which serve as the alphabet for the language of life, are represented by just four letters: A, C, G, and T, corresponding to adenine, cytosine, guanine, and thymine. The nucleotide alphabet codes for the sequence ...
... nucleotides, which are organized into DNA molecules—the double helix. The nucleotides, which serve as the alphabet for the language of life, are represented by just four letters: A, C, G, and T, corresponding to adenine, cytosine, guanine, and thymine. The nucleotide alphabet codes for the sequence ...
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
... relative to the strength of the promoter— that occurs in these areas, the greater the chance that low or no transcription will occur (Phillips). This repression is greater if the promoter itself is methylated, but distantly methylated sequences can also contribute to repression —especially if the me ...
... relative to the strength of the promoter— that occurs in these areas, the greater the chance that low or no transcription will occur (Phillips). This repression is greater if the promoter itself is methylated, but distantly methylated sequences can also contribute to repression —especially if the me ...
Estrogen receptor β gene polymorphisms and susceptibility to
... leiomyomas when compared with autologous myometrial tissue [7–11]. In 1996, the second estrogen receptor, ERb, was identified which is coded by the ESR2 gene. Like ERa, ERb also was reported to be expressed at higher levels in leiomyomas when compared with matched myometrium [12]. There are studies ...
... leiomyomas when compared with autologous myometrial tissue [7–11]. In 1996, the second estrogen receptor, ERb, was identified which is coded by the ESR2 gene. Like ERa, ERb also was reported to be expressed at higher levels in leiomyomas when compared with matched myometrium [12]. There are studies ...
Genomic surveys and expression analysis of bZIP gene family in
... and oligo (dT) used as the primers to synthesize the first and second-strand cDNA. The cDNA was digested by two types of endonuclease NlaIII or DpnII, acquiring 17 bp tags with different adaptors of both ends to generate a tag library. After 15 cycles of linear PCR amplification, 105 bp fragments we ...
... and oligo (dT) used as the primers to synthesize the first and second-strand cDNA. The cDNA was digested by two types of endonuclease NlaIII or DpnII, acquiring 17 bp tags with different adaptors of both ends to generate a tag library. After 15 cycles of linear PCR amplification, 105 bp fragments we ...
mtr function Background Luis M. Corrochano
... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
BSC Yeast Genetics I
... Very similar to bacteria (prokaryotes) that you have previously used in other labs, yeast (eukaryotes) cells have similar nutritional requirements and optimal temperatures for growth. Yeast can be grown on solid agar media on a petri dish, or in liquid cultures in a shaking water bath. Yeast have a ...
... Very similar to bacteria (prokaryotes) that you have previously used in other labs, yeast (eukaryotes) cells have similar nutritional requirements and optimal temperatures for growth. Yeast can be grown on solid agar media on a petri dish, or in liquid cultures in a shaking water bath. Yeast have a ...
Coordination of replication and transcription along a Drosophila
... hybridized labeled poly(A)-containing mRNA from asynchronous cells to the genomic microarray, using total genomic DNA as a control. The resulting pattern of expression along the chromosome was in close agreement with the predictions of inter- and intragenic sequences from the Drosophila annotation p ...
... hybridized labeled poly(A)-containing mRNA from asynchronous cells to the genomic microarray, using total genomic DNA as a control. The resulting pattern of expression along the chromosome was in close agreement with the predictions of inter- and intragenic sequences from the Drosophila annotation p ...
Gene Section SIX1 (sine oculis homeobox homolog 1) (mammalian)
... RPMI-324B11 (labeled in SpectrumGreen) mapped at 14q11.2 was used as control. Metaphase spread (B) and interphase nuclei (C) of the non-malignant, immortalized MCF10A cell line showing two normal copies of chromosome 14 with signals generated by the SIX1 and control FISH probes. Metaphase spread of ...
... RPMI-324B11 (labeled in SpectrumGreen) mapped at 14q11.2 was used as control. Metaphase spread (B) and interphase nuclei (C) of the non-malignant, immortalized MCF10A cell line showing two normal copies of chromosome 14 with signals generated by the SIX1 and control FISH probes. Metaphase spread of ...
Supplementary Data File Supplementary Figures Figure S1
... reference distribution corresponds to a hypothetical data set with 10,000 genes where 2,500 genes are in the low variability category, 5000 in the medium and 2500 in the high variability category. B. The count distribution of Pathway 1 contains a total of 100 genes from the dataset. From these 100 g ...
... reference distribution corresponds to a hypothetical data set with 10,000 genes where 2,500 genes are in the low variability category, 5000 in the medium and 2500 in the high variability category. B. The count distribution of Pathway 1 contains a total of 100 genes from the dataset. From these 100 g ...
Overexpression of the catalytic subunit of DNA polymerase results in
... There are enormous differences in the mitochondrial content of the different tissues of the organism, and a precise program of organelle biogenesis is needed during development. Recently, mitochondrial transcription factor A (Tfam) has been shown to be essential for mitochondrial biogenesis. Disrup ...
... There are enormous differences in the mitochondrial content of the different tissues of the organism, and a precise program of organelle biogenesis is needed during development. Recently, mitochondrial transcription factor A (Tfam) has been shown to be essential for mitochondrial biogenesis. Disrup ...
Argonaute2 Is Essential for Mammalian
... embryos exhibit an expansion of the primitive streak (block-arrow). Note that Ago2–/– e7.5 embryos are smaller and rounder than wild-type, suggesting aberrant growth. The scale bar represents 200 lm. (A, B, insets) Sections from whole-mount in situ hybridized e7.5 embryos. Shown are representative w ...
... embryos exhibit an expansion of the primitive streak (block-arrow). Note that Ago2–/– e7.5 embryos are smaller and rounder than wild-type, suggesting aberrant growth. The scale bar represents 200 lm. (A, B, insets) Sections from whole-mount in situ hybridized e7.5 embryos. Shown are representative w ...
Unverified Color Pink-eye dilution Ukraine
... single gene. We have started to search for this gene and for the causative mutation, thanks to cheek-‐swab samples from “pink-‐eyed” cats that were sent by owners and breeders. DNA ...
... single gene. We have started to search for this gene and for the causative mutation, thanks to cheek-‐swab samples from “pink-‐eyed” cats that were sent by owners and breeders. DNA ...
RT2 Profiler PCR Arrays: Pathway-focused gene
... disease state. Wells H1 through H5 contain a panel of housekeeping genes (HK1–HK5) used for normalizing the RT2 Profiler PCR Array data. Well H6 contains a Genomic DNA Control (GDC) primer set that specifically detects nontranscribed, repetitive genomic DNA with a high level of sensitivity. Wells H7 ...
... disease state. Wells H1 through H5 contain a panel of housekeeping genes (HK1–HK5) used for normalizing the RT2 Profiler PCR Array data. Well H6 contains a Genomic DNA Control (GDC) primer set that specifically detects nontranscribed, repetitive genomic DNA with a high level of sensitivity. Wells H7 ...
Characterization of the wheat gene encoding a grain
... Some LTPs were found to be expressed in several grain tissues. For example, two grain-specific maize genes of unknown function, ZmEBE-1 and ZmEBE-2, were found to be expressed in the embryo sac before pollination and later after pollination in both ETC and ESR cells of the developing endosperm (Magn ...
... Some LTPs were found to be expressed in several grain tissues. For example, two grain-specific maize genes of unknown function, ZmEBE-1 and ZmEBE-2, were found to be expressed in the embryo sac before pollination and later after pollination in both ETC and ESR cells of the developing endosperm (Magn ...
Screening Mutant Libraries of Fungal Laccases in the Presence of
... Next to Escherichia coli, S. cerevisiae is the most commonly used host organism in laboratory evolution. Its sophisticated cellular machinery simplifies mutant library generation. By homologous recombination, otherwise cumbersome ligation of mutant genes into expression vectors or shuffling of error ...
... Next to Escherichia coli, S. cerevisiae is the most commonly used host organism in laboratory evolution. Its sophisticated cellular machinery simplifies mutant library generation. By homologous recombination, otherwise cumbersome ligation of mutant genes into expression vectors or shuffling of error ...
Transgenic approaches for optogenetics Tim Murphy UBC.
... Cre is often expressed from a randomly integrated transgene, but very few insertion sites are known. Random transgene integration can disrupt an endogenous mouse gene and cause unanticipated side effects. Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygou ...
... Cre is often expressed from a randomly integrated transgene, but very few insertion sites are known. Random transgene integration can disrupt an endogenous mouse gene and cause unanticipated side effects. Since hemizygous mice have a wildtype chromosome present, using hemizygous instead of homozygou ...
CapeTownGenomes
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
... Few genomes are completely sequenced. The completion and quality assurance needed for bacterial genomes is expensive, for larger eukaryotes even more so. ‘Finishing’ is the process by which a WGS shotgun assembly is completed (determine the sequence from any physical or sequence gaps) and furthe ...
The role of sex chromosomes in mammalian germ cell differentiation
... be detrimental in humans, but less so in mice. However, it was recently proposed that human XO embryos may die due to a haplodeficiency of placental gene expression.49 If this hypothesis is correct, the early loss of XO oocytes may be a consequence of placental defects, and they may survive and beco ...
... be detrimental in humans, but less so in mice. However, it was recently proposed that human XO embryos may die due to a haplodeficiency of placental gene expression.49 If this hypothesis is correct, the early loss of XO oocytes may be a consequence of placental defects, and they may survive and beco ...
Effects of OPRM1, ABCB1 and CYP2D6 single nucleotide
... OPRM1: Opioid receptor mu 1; ABCBl: ATP-binding cassette B1; CYP2D6: Cytochrome P450 consists of 28 exons 2D6; F: forward; R: reverse. [17, 18]. Cytochrome P450 2D6 (CYP2D6) is deemed as one of the most important enzymes related to the metabolism of xenobiotics in the body [19]. The CYP2D6 gene (sub ...
... OPRM1: Opioid receptor mu 1; ABCBl: ATP-binding cassette B1; CYP2D6: Cytochrome P450 consists of 28 exons 2D6; F: forward; R: reverse. [17, 18]. Cytochrome P450 2D6 (CYP2D6) is deemed as one of the most important enzymes related to the metabolism of xenobiotics in the body [19]. The CYP2D6 gene (sub ...
Molecular Mechanisms of Plant and Microbe Coexistence
... whose response is controlled by the expression of global regulatory genes (regulons), have been also used to monitor microbial activity as affected by fluctuations of temperature, osmolarity, moisture, radicals concentrations, etc in the rhizosphere soil in soil due to plant activity (van Dyk et al ...
... whose response is controlled by the expression of global regulatory genes (regulons), have been also used to monitor microbial activity as affected by fluctuations of temperature, osmolarity, moisture, radicals concentrations, etc in the rhizosphere soil in soil due to plant activity (van Dyk et al ...
Foundations of Biology - Geoscience Research Institute
... genes in amitochondrial eukaryotes all reinterpreted prevailing theory in saying that mitochondria must have been present then lost after they had transferred some of their genetic information to the nucleus. The hydrogenosome, a structure involved in carbohydrate metabolism found in some Archaezoan ...
... genes in amitochondrial eukaryotes all reinterpreted prevailing theory in saying that mitochondria must have been present then lost after they had transferred some of their genetic information to the nucleus. The hydrogenosome, a structure involved in carbohydrate metabolism found in some Archaezoan ...
lecture - Berkeley MCB
... Note: this diagram is not entirely correct (in an entirelly incorrect sort of way), because the estrogen receptor (ER), a class I nuclear hormone receptor, is not typically bound to target genes in the absence of hormone – such behavior is characteristic of class II nuclear hormone receptors, such ...
... Note: this diagram is not entirely correct (in an entirelly incorrect sort of way), because the estrogen receptor (ER), a class I nuclear hormone receptor, is not typically bound to target genes in the absence of hormone – such behavior is characteristic of class II nuclear hormone receptors, such ...
Real time PCR based determination of gene copy numbers in
... integration is possible, complicating the molecular analysis. To overcome these drawbacks and also in order to deal with increasing numbers of expression strains from high throughput experiments, an additional reliable and faster method for copy number determination of integrated expression cassette ...
... integration is possible, complicating the molecular analysis. To overcome these drawbacks and also in order to deal with increasing numbers of expression strains from high throughput experiments, an additional reliable and faster method for copy number determination of integrated expression cassette ...
1 Haploinsufficient loss of multiple 5q genes may
... Clinical-cytogenetic associations in 306 patients with therapy-related myelodysplasia and myeloid leukemia: the University of Chicago series. Blood. 2003;102(1):43-52. ...
... Clinical-cytogenetic associations in 306 patients with therapy-related myelodysplasia and myeloid leukemia: the University of Chicago series. Blood. 2003;102(1):43-52. ...
Tools for functional annotation
... 3. Example: The AgBase GO annotation pipeline 4. Other GO annotation tools ...
... 3. Example: The AgBase GO annotation pipeline 4. Other GO annotation tools ...