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Long period grating based biosensor for the detection of Escherichia
Long period grating based biosensor for the detection of Escherichia

... Several schemes based on the label-free methods, like evanescent wave coupling in grating couplers (Horváth et al., 2003), leaky optical waveguides (Zourob et al., 2005) and SPR (Bae et al., 2004; Oh et al., 2005; Taylora et al., 2005; Homola, 2006) for the detection of E. coli have been proposed in ...
Fluorescence Microscopy
Fluorescence Microscopy

... blue light also has the disadvantage that is more likely to damage cells. Following the concept that red light is scattered less, two-photon excitation of thick tissues is best coupled with an indicator that fluoresces and emits photons in the red region. In conventional imaging, spatial resolution i ...
Extended depth-of-field iris recognition system for a
Extended depth-of-field iris recognition system for a

... field of view determines the spatial extent of the scene acquired by the sensor. Depth of field determines how far a planar object can move away from the best focus position and still be imaged without focus errors. The exposure period is defined as the detector integration time while acquiring an i ...
Changes in spatial extent and peak double optical density of human
Changes in spatial extent and peak double optical density of human

... The macular pigment (MP) is a yellow pigment found in the human fovea in both the inner and outer layers of the retina, and located along the axons of the cone photoreceptors [1]. It is mainly composed of two xanthophylls, lutein and zeaxanthin [2]. MP has a peak absorption wavelength at 460 nm but ...
Saturation
Saturation

... Since the absorption profile of a homogeneously broadened line is Lorentzian, the induced absorption probability is B12  ( ) L(  0 ) And the saturation parameter is B12  ( ) S  L(   0 )  S ( 0 ) ...
Optimization of axial resolution in a confocal microscope with
Optimization of axial resolution in a confocal microscope with

... of detector radius νd , with values of d of 0, 0.3, 0.5, and also for circular pupils. It is seen that for a given value of d, the signal level increases as the size of the detector increases. However, for a given radius νd of the detector, the signal level decreases monotonically as d increases. Fo ...
Chapter 2 Femtosecond Laser Pulses - diss.fu
Chapter 2 Femtosecond Laser Pulses - diss.fu

... the set of longitudinal modes of the laser. For the stronger focused frequencies, the Kerr lens favors a higher amplification. Thus the self-focusing of the seed beam can be used to suppress the cw operation, because the losses of the cw radiation are higher. Forcing all the modes to have equal phas ...
Graded Index Fiber
Graded Index Fiber

...  Graded index fiber do not have a constant refractive index in a fiber core.  This index variation represented as n(r) = n1(1 − 2Δ(r/a)α)1/2 , r < a (core) n1(1 − 2Δ)1/2 = n2 , r ≥ a (cladding) Where, Δ is the relative refractive index difference α is the profile parameter, which gives the charact ...
Biology 177: Principles of Modern Microscopy
Biology 177: Principles of Modern Microscopy

... • Technique that dominates most single molecule imaging approaches ...
Wavefront Technology
Wavefront Technology

... used in ocular aberrometers is to measure the deviation of individual rays of light passing through various locations in the pupil of the eye. This is done by measuring reflected light from the retinal spot created by the central reference beam. This spot serves as a point source that radiates light ...
Introduction to Aberrations OPTI 518 Jose Sasian
Introduction to Aberrations OPTI 518 Jose Sasian

Paraxial Optics
Paraxial Optics

... geometrical optics.[1–11] Other textbooks focused in geometrical optics propose that the paraxial treatment should be included as part of the first approach to the subject.[12–16] The paraxial approximation explains how light propagates along an optical system when rays are close to the optical axis ...
[pdf]
[pdf]

- ANU Repository
- ANU Repository

... Fig. 2(a). It is clear that although both dominant ED and MD are supported with resonances centered at point E and M, respectively, they are spectrally separated. Above Fig. 2(a) we also show the near-field distributions of longitudinal magnetic field intensity (|Hz |2 ,colormap) and transverse electr ...
Generation and Application of Bessel Beams in Electron Microscopy
Generation and Application of Bessel Beams in Electron Microscopy

... potentially reaching efficiencies as large as 100% [5,6]. Here, we report a detailed study of electron Bessel beam generation using this technique. Bessel beams are widely used in photonics, and have recently been discussed theoretically in the context of a number of electron microscopy application ...
Method and apparatus for gas discharge laser output light
Method and apparatus for gas discharge laser output light

... suring the bandWidths of an output laser beam comprising output laser light in the range of beloW 500 femtometers at accuracies Within tens of femtometers. The homogeniZer may comprise a rotating diffuser Which may be a ground glass diffuser Which may also be etched. The Wavemeter may also comprise ...
PDF
PDF



502-22 Illumination Systems
502-22 Illumination Systems

... The filament in a light bulb is a 3D structure. It is often a coil of a coil of tungsten wire. Because of this structure, the filament itself may shadow other parts of the filament. This filament shadowing can result in non-uniform illumination. ...
Parallelized STED fluorescence nanoscopy
Parallelized STED fluorescence nanoscopy

... Since the beginning of the 20th century, far-field optical microscopy has been essentially limited to >λ/2 > 200 nm in resolution, with λ denoting the wavelength of light. While electron and scanning probe microscopy have provided much finer details since then, they lack the possibility of non-invas ...
Jamie Ball, Retrieving the complex refractive index of volcanic ash
Jamie Ball, Retrieving the complex refractive index of volcanic ash

... formed at three wavelengths: 450.0 nm, 546.7 nm lower refractive index and the above process would and 650.0 nm. This was accomplished by placing have been repeated. If the Becke line moved from optical filters, with a bandpass of ±10 nm about the the liquid into the fragments, the ash was noted to ...
Ultrafast Laser-induced Damage and the Influence of Spectral Effects
Ultrafast Laser-induced Damage and the Influence of Spectral Effects

... field with a wavelength of 800 nm, as well as those for many neighboring wavelengths. Note that the 775, 800, and 825 nm wavelength rates are in good agreement for intensities in the MPI limit. This indicates that photoionization for the 140 fs laser pulse shown in Fig. 1, ranging spectrally from 79 ...
Representation Of A Nonspherical Ice Particle By A
Representation Of A Nonspherical Ice Particle By A

... (NGW03, their Figure 7). This ‘‘equidimensional aberration’’ decreases for both larger and smaller aspect ratios (columns and plates respectively). For the example just cited, gs  gx switches from positive to negative for hexagonal prisms with aspect ratios of approximately 1/10 (plates) and 10 (co ...
Lab 4: Diffraction of Light
Lab 4: Diffraction of Light

... Exercise 5: Slide each slit into the laser beam, and measure the width of the central intensity maximum. How does the single slit diffraction pattern change as the width of the slit increases? Calculate the average value of the wavelength of the laser using your Exercise 1: Sketch the pattern that y ...
Symbolic computation and solitons of the nonlinear Schro¨dinger
Symbolic computation and solitons of the nonlinear Schro¨dinger

... Remark: (1) When setting C1 = 0, M(z) = 0, the Theorems 1–2 [17] and Theorems 1–2 [18] can be reproduced by our solutions (11) and (12); (2) When setting M(z) = 0, the solutions obtained in [23] can also be recovered by selecting arbitrary functions and arbitrary constants suitably; (3) The solution ...
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Optical tweezers



Optical tweezers (originally called ""single-beam gradient force trap"") are scientific instruments that use a highly focused laser beam to provide an attractive or repulsive force (typically on the order of piconewtons), depending on the refractive index mismatch to physically hold and move microscopic dielectric objects similar to tweezers. Optical tweezers have been particularly successful in studying a variety of biological systems in recent years.
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