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Transcription and Translation
Transcription and Translation

... • Each group of 3 nucleotides on the mRNA is a codon. Since there are 4 bases, there are 43 = 64 possible codons, which must code for 20 different amino acids. • More than one codon is used for most amino acids: the genetic code is “degenerate”. This means that it is not possible to take a protein s ...
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... 5. Peptide bond forms between the ______ amino acids 6. Ribosome ________________ the ________ from the ________ 7. The tRNA ______________ away from the ribosome, leaving the _____________ ...
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...  Splicing process may help in regulating the export of mRNA into the cytoplasm  May allow single gene to direct the synthesis of different proteins  Eveolution protein diversity  Increse the probability that recombination of exons will occur between alleles Translation: synthesis of a polypeptid ...
Biology: Life on Earth
Biology: Life on Earth

... repressor protein bound to operator, overlaps promoter free repressor proteins When lactose is not present, repressor proteins bind to the operator of the lactose operon. When RNA polymerase binds to the promoter, the repressor protein blocks access to the structural genes, which therefore cannot be ...
Nucleic Acids and the Genetic Code
Nucleic Acids and the Genetic Code

... tRNA molecules attach to the ribosome, and their anticodons pair up with the appropriate codons on the mRNA. The amino acids transported by the tRNA link together, and the tRNA molecules then return to the cytoplasm. The ribosome moves along the mRNA, and amino acids continue to join together until ...
Biology 10.2 Review Genes to Proteins
Biology 10.2 Review Genes to Proteins

... transcription factor (called an activator) into contact with the transcription factors and RNA polymerase at the promoter. Transcription factors bound to enhancers can activate transcription factors bound to promoters. ...
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... transcription factor (called an activator) into contact with the transcription factors and RNA polymerase at the promoter. Transcription factors bound to enhancers can activate transcription factors bound to promoters. ...
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... processed into 28S, 5.8S, and 18S rRNAs – RNA polymerase III: synthesis of tRNA, 18 S rRNA, and small, stable RNAs – RNA polymerase II: synthesis of mRNAs and four small nuclear RNAs that take part in RNA splicing ...
STUDY GUIDE for Dr. Mohnen`s part of Exam #3
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Complete Protocol

... The FluoroTect™ GreenLys in vitro Translation Labeling System(a) allows fluorescent labeling of in vitro translation products through the use of a modified charged lysine transfer RNA labeled with the fluorophore BODIPY®-FL. Using this system, fluorescently labeled lysine residues are incorporated into ...
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... specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and substitutes the nitrogenous base uracil for thymine. – An RNA molecules almost always consists of a single strand. Copyright © 2002 Pearson Ed ...
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... One of the most interesting discoveries of molecular biology is the nearuniversal nature of the genetic code. Although some organisms show slight variations in the amino acids assigned to particular codons, the code is always read three bases at a time and in the same direction. Despite their enormo ...
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... exhibiting sequence similarity to known bacterial, archaeal, and eukaryotic ribosomal protein encoding genes has been identified. In general, the archaeal r-proteins are more similar in sequence to their eucaryal than to their bacterial homologs. However, as in bacteria, 53 of the 60 genes are in 15 ...
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... throughout the cell cycle with the exception of mitosis • Occurs in the nucleus as messenger RNA copies DNA ...
workshop module 6: dna, rna and proteins - Peer
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... contain the same genetic information. This process is called DNA replication. Aside from cell division, the cell also has to make copies of DNA in order to make proteins (DNA is like the recipe, and the protein is like the final dish). To make a protein, the cell must first make a complementary copy ...
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...  Ribosomal RNA (rRNA) - constitutes about 65% of the material in ribosomes, the sites of protein synthesis ► Ribosome - a sub cellular particle that serves as the site of protein synthesis in all organisms ...
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...  Single stranded molecule with attachment site at one end for an amino acid  Opposite end has three nucleotide bases called the anticodon ...
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... 22. This type of RNA, along with proteins, makes up the structure of a ribosome rRNA. 23. Which organelle makes proteins when its rRNA moves along the mRNA and reads the message during protein synthesis? Ribosome 24. Which type of RNA carries specific amino acids to the ribosome to be put in the cor ...
Chapter 12 Test Review
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... 22. This type of RNA, along with proteins, makes up the structure of a ribosome rRNA. 23. Which organelle makes proteins when its rRNA moves along the mRNA and reads the message during protein synthesis? Ribosome 24. Which type of RNA carries specific amino acids to the ribosome to be put in the cor ...
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... • tRNA (transfer RNA) • mRNA (messenger RNA) The role of RNA : • as a structural molecule (rRNA), • as an information transfer molecule (mRNA), • as an information decoding molecule (tRNA) The structural, informational transfer and information adaptor roles of RNA are all involved in decoding the in ...
Genes Expression or Genes and How They Work: Transcription
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... embedded within stretches of __________________________ called introns. The initial messenger RNA molecule or _________________________ copied from a gene by RNA polymerase, is a faithful copy of ________________, including _______________________. Before the primary transcript is ________________, ...
X-Sheet 2 Protein Synthesis and DNA Fingerprinting
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... DNA probes. A selection of DNA sequences within the DNA profile forms what is termed the VNTR pattern for that individual. Forensic scientists are able to compare the DNA profiles to a sample that is provided from a crime scene. DNA profiling is very accurate. Sequencing of DNA: A species is a group ...
The Genetic Code: The most fundamental concept in all biology
The Genetic Code: The most fundamental concept in all biology

... The Genetic Code: The most fundamental concept in all biology. Shortly after the discovery of the structure of DNA by Watson and Crick, scientists were faced with a significant problem: How does the information encoded in DNA get translated into a protein. In 1954, our old friend Francis Crick hypot ...
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Transfer RNA



A transfer RNA (abbreviated tRNA and archaically referred to as sRNA, for soluble RNA) is an adaptor molecule composed of RNA, typically 76 to 90 nucleotides in length, that serves as the physical link between the mRNA and the amino acid sequence of proteins. It does this by carrying an amino acid to the protein synthetic machinery of a cell (ribosome) as directed by a three-nucleotide sequence (codon) in a messenger RNA (mRNA). As such, tRNAs are a necessary component of translation, the biological synthesis of new proteins according to the genetic code.The specific nucleotide sequence of an mRNA specifies which amino acids are incorporated into the protein product of the gene from which the mRNA is transcribed, and the role of tRNA is to specify which sequence from the genetic code corresponds to which amino acid. One end of the tRNA matches the genetic code in a three-nucleotide sequence called the anticodon. The anticodon forms three base pairs with a codon in mRNA during protein biosynthesis. The mRNA encodes a protein as a series of contiguous codons, each of which is recognized by a particular tRNA. On the other end of the tRNA is a covalent attachment to the amino acid that corresponds to the anticodon sequence. Each type of tRNA molecule can be attached to only one type of amino acid, so each organism has many types of tRNA (in fact, because the genetic code contains multiple codons that specify the same amino acid, there are several tRNA molecules bearing different anticodons which also carry the same amino acid).The covalent attachment to the tRNA 3’ end is catalyzed by enzymes called aminoacyl tRNA synthetases. During protein synthesis, tRNAs with attached amino acids are delivered to the ribosome by proteins called elongation factors (EF-Tu in bacteria, eEF-1 in eukaryotes), which aid in decoding the mRNA codon sequence. If the tRNA's anticodon matches the mRNA, another tRNA already bound to the ribosome transfers the growing polypeptide chain from its 3’ end to the amino acid attached to the 3’ end of the newly delivered tRNA, a reaction catalyzed by the ribosome.A large number of the individual nucleotides in a tRNA molecule may be chemically modified, often by methylation or deamidation. These unusual bases sometimes affect the tRNA's interaction with ribosomes and sometimes occur in the anticodon to alter base-pairing properties.
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