Inducing Embryonic Stem Cells to Become
... ICM derivatives exhibiting a very dense, refractile morphology. Clusters of refractile cells were then physically isolated, dispersed, and replated onto MEF feeder layers. This process was repeated until clonal ESC lines were established (Fig. 1e). Mouse ESC lines can be propagated extensively in an ...
... ICM derivatives exhibiting a very dense, refractile morphology. Clusters of refractile cells were then physically isolated, dispersed, and replated onto MEF feeder layers. This process was repeated until clonal ESC lines were established (Fig. 1e). Mouse ESC lines can be propagated extensively in an ...
Organization of the Animal Body
... thus provide a barrier that can impede the passage of some substances while facilitating the passage of others. For land-dwelling vertebrates, the relative impermeability of the surface epithelium (the epidermis) to water offers essential protection from dehydration and from airborne pathogens (dise ...
... thus provide a barrier that can impede the passage of some substances while facilitating the passage of others. For land-dwelling vertebrates, the relative impermeability of the surface epithelium (the epidermis) to water offers essential protection from dehydration and from airborne pathogens (dise ...
Editas Medicine Presents Data from Multiple Therapeutic Discovery
... deliver CRISPR/Cas9 molecules to primary human hematopoietic stem cells (HSCs). For selected approaches, Cas9 gene-edited HSCs maintained both viability and ex vivo hematopoietic colony-forming potential, similar to unedited cells. ...
... deliver CRISPR/Cas9 molecules to primary human hematopoietic stem cells (HSCs). For selected approaches, Cas9 gene-edited HSCs maintained both viability and ex vivo hematopoietic colony-forming potential, similar to unedited cells. ...
File
... which is formed by the union of an egg and sperm. The zygote divides and differentiates into more than 200 different types of human cells. These cells allow you to do everything from lifting a glass, to learning people’s names, to maintaining your body temperature on a cold day. Cell specialization ...
... which is formed by the union of an egg and sperm. The zygote divides and differentiates into more than 200 different types of human cells. These cells allow you to do everything from lifting a glass, to learning people’s names, to maintaining your body temperature on a cold day. Cell specialization ...
activities - Linn-Benton Community College
... Membranes that cover and line Everything that enters or leaves body passes through or between epithelial cells ...
... Membranes that cover and line Everything that enters or leaves body passes through or between epithelial cells ...
The Hydra polyp: Nothing but an active stem cell community
... and plasticity of their cells. Vertebrates depend on specialized cells with limited differentiation potential to perform sophisticated functions. In contrast, even adult tissue cells in Hydra are capable of producing and ...
... and plasticity of their cells. Vertebrates depend on specialized cells with limited differentiation potential to perform sophisticated functions. In contrast, even adult tissue cells in Hydra are capable of producing and ...
The Human Body: An Orientation
... • Chemical level—atoms form molecules • Cellular level—cells and their functional subunits • Tissue level—a group of cells performing a common function • Organ level—a discrete structure made up of more than one tissue • Organ system—organs working together for a common purpose • Organism level—the ...
... • Chemical level—atoms form molecules • Cellular level—cells and their functional subunits • Tissue level—a group of cells performing a common function • Organ level—a discrete structure made up of more than one tissue • Organ system—organs working together for a common purpose • Organism level—the ...
The Role of the Leukemia Inhibitory Factor (LIF)
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
... the LIF/STAT3-pathway in vivo. However, studies on knockout mouse models indicate that at least the early embryonic development can occur also in the absence of some components of the LIF-pathway but many of these mouse models then display later on during development or during adulthood many differe ...
Practice Exam 5
... 20) High-flying birds are able to obtain enough oxygen even when the air is very thin because a. they have more efficient lungs than other vertebrates. b. they have reduced amounts of hemoglobin in their blood. c. their mitochondria are more efficient than those of other vertebrates. d. their heart ...
... 20) High-flying birds are able to obtain enough oxygen even when the air is very thin because a. they have more efficient lungs than other vertebrates. b. they have reduced amounts of hemoglobin in their blood. c. their mitochondria are more efficient than those of other vertebrates. d. their heart ...
Intrahepatic expression of the hepatic stellate cell marker ®broblast
... Abstract: Background: Activated hepatic stellate cells (HSCs), recognised by their a smooth muscle actin immunoreactivity, are primarily responsible for liver ®brosis. However, the presence of a smooth muscle actin positive HSCs is not always associated with the development of liver ®brosis. Recentl ...
... Abstract: Background: Activated hepatic stellate cells (HSCs), recognised by their a smooth muscle actin immunoreactivity, are primarily responsible for liver ®brosis. However, the presence of a smooth muscle actin positive HSCs is not always associated with the development of liver ®brosis. Recentl ...
Differentiation of Mesenchymal Stem Cells Into Dopaminergic
... vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the m ...
... vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the m ...
DNA Methylation Profiles Define Stem Cell Identity and
... profiles identify a cohort of developmentally regulated sequence elements, such as orphan CpG islands, that will be most valuable to uncover novel transcriptional regulators and pivotal ‘‘gatekeeper’’ genes in pluripotency and lineage differentiation. STEM CELLS 2012;30:2732–2745 ...
... profiles identify a cohort of developmentally regulated sequence elements, such as orphan CpG islands, that will be most valuable to uncover novel transcriptional regulators and pivotal ‘‘gatekeeper’’ genes in pluripotency and lineage differentiation. STEM CELLS 2012;30:2732–2745 ...
Ontogeny of erythropoiesis
... mature in a synchronous cohort as they undergo changes well recognized in maturing definitive erythroid precursors, including a limited number of cell divisions, accumulation of increasing amounts of hemoglobin, nuclear condensation, a progressive decrease in cell size, and ultimately enucleation [3 ...
... mature in a synchronous cohort as they undergo changes well recognized in maturing definitive erythroid precursors, including a limited number of cell divisions, accumulation of increasing amounts of hemoglobin, nuclear condensation, a progressive decrease in cell size, and ultimately enucleation [3 ...
Multipotent stem cells from the dorsal aorta
... developing tissues, vessel-associated progenitors, which possibly originate from a common ancestor (a ‘mesoangioblast’, rather than an hemo-angioblast) would leave the vessel and adopt the fate of the tissue where the vessel has entered (Bianco and Cossu, 1999). This fate choice would depend upon lo ...
... developing tissues, vessel-associated progenitors, which possibly originate from a common ancestor (a ‘mesoangioblast’, rather than an hemo-angioblast) would leave the vessel and adopt the fate of the tissue where the vessel has entered (Bianco and Cossu, 1999). This fate choice would depend upon lo ...
Dendritic Cell Development in Long
... are three known subsets in spleen [17], two in thymus [17], and five in lymph node [18]. More recently, the distinct lineage of murine plasmacytoid DCs was identified in multiple organs on the basis of B220 expression [19]. DC subsets in murine spleen are located in two distinct locations: some are ...
... are three known subsets in spleen [17], two in thymus [17], and five in lymph node [18]. More recently, the distinct lineage of murine plasmacytoid DCs was identified in multiple organs on the basis of B220 expression [19]. DC subsets in murine spleen are located in two distinct locations: some are ...
Bone marrow side population cells are enriched for progenitors
... Fig. 2. WBM and SP transplantations result in equivalent hematopoietic lineage reconstitution. Markers for B cell (B220), T lymphocyte (CD3), mature granulocyte (Gr-1), monocyte/macrophage (Mac-1) and erythroid (Ter119) lineages were tested using a mouse lineage panel. For each marker, the percentag ...
... Fig. 2. WBM and SP transplantations result in equivalent hematopoietic lineage reconstitution. Markers for B cell (B220), T lymphocyte (CD3), mature granulocyte (Gr-1), monocyte/macrophage (Mac-1) and erythroid (Ter119) lineages were tested using a mouse lineage panel. For each marker, the percentag ...
h) Transport - iGCSE Science Courses
... structure and the presence of haemoglobin, make them suitable for the transport of oxygen 2.60 describe how the immune system responds to disease using white blood cells, illustrated by phagocytes ingesting pathogens and lymphocytes releasing antibodies specific to the pathogen 2.61 understand that ...
... structure and the presence of haemoglobin, make them suitable for the transport of oxygen 2.60 describe how the immune system responds to disease using white blood cells, illustrated by phagocytes ingesting pathogens and lymphocytes releasing antibodies specific to the pathogen 2.61 understand that ...
Active tissue-specific DNA demethylation conferred by somatic cell
... muscle cells with human primary keratinocytes derived from neonatal foreskin. In this heterokaryon system, differentiating myoblasts exit the cell cycle and fuse with each other to form multinucleated myotubes, to which human keratinocytes are fused by using PEG. Cultures are treated with the agents ...
... muscle cells with human primary keratinocytes derived from neonatal foreskin. In this heterokaryon system, differentiating myoblasts exit the cell cycle and fuse with each other to form multinucleated myotubes, to which human keratinocytes are fused by using PEG. Cultures are treated with the agents ...
Hepatosplenic gamma/delta T-Cell Lymphoma in Bone Marrow A
... dermis ❚Image 5B❚. The atypical lymphocytes were medium to large with dense, clumped chromatin; inconspicuous nucleoli; irregular (but not cerebriform) nuclear contours; and scant cytoplasm. The infiltrate lacked admixed inflammatory cells. An autopsy performed in case 3 revealed neoplastic cells wi ...
... dermis ❚Image 5B❚. The atypical lymphocytes were medium to large with dense, clumped chromatin; inconspicuous nucleoli; irregular (but not cerebriform) nuclear contours; and scant cytoplasm. The infiltrate lacked admixed inflammatory cells. An autopsy performed in case 3 revealed neoplastic cells wi ...
Stem cells as a source of insulin
... etc. Other terms such as progenitor or precursor are used to indicate that these cells have lower ability to renew themselves and more narrow range of differentiation. Stem cells are identified and characterised by expression of markers which are not expressed in end-differentiated cells and which m ...
... etc. Other terms such as progenitor or precursor are used to indicate that these cells have lower ability to renew themselves and more narrow range of differentiation. Stem cells are identified and characterised by expression of markers which are not expressed in end-differentiated cells and which m ...
THE RESPIRATORY SYSTEM
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
Lesson 1
... • This deoxygenated blood enters the right atrium and is transferred to the right ventricle. • The blood is then pumped to the lungs. • The blood releases carbon dioxide and picks up oxygen from inhaled air and returns to the left atrium of the heart. • The left atrium pumps the oxygenated blood int ...
... • This deoxygenated blood enters the right atrium and is transferred to the right ventricle. • The blood is then pumped to the lungs. • The blood releases carbon dioxide and picks up oxygen from inhaled air and returns to the left atrium of the heart. • The left atrium pumps the oxygenated blood int ...
Lesson 1 cardiovascular system
... • This deoxygenated blood enters the right atrium and is transferred to the right ventricle. • The blood is then pumped to the lungs. • The blood releases carbon dioxide and picks up oxygen from inhaled air and returns to the left atrium of the heart. • The left atrium pumps the oxygenated blood int ...
... • This deoxygenated blood enters the right atrium and is transferred to the right ventricle. • The blood is then pumped to the lungs. • The blood releases carbon dioxide and picks up oxygen from inhaled air and returns to the left atrium of the heart. • The left atrium pumps the oxygenated blood int ...
THE RESPIRATORY SYSTEM
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
THE RESPIRATORY SYSTEM
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
... Each alveoli wall usually lies between two neighbouring alveoli and is called an inter-alveolar septum. An alveolar septum consists of two thin squamous epithelial layers between which lie capillaries, fibroblasts, elastic and reticular fibers and macrophages. The capillaries and the connectiv ...
Hematopoietic stem cell
Hematopoietic stem cells (HSCs) are the blood cells that give rise to all the other blood cells and are derived from mesoderm. They are located in the red bone marrow, which is contained in the core of most bones.They give rise to both the myeloid and lymphoid lineages of blood cells. (Myeloid cells include monocytes, macrophages, neutrophils, basophils, eosinophils, erythrocytes, dendritic cells, and megakaryocytes or platelets. Lymphoid cells include T cells, B cells, and natural killer cells.) The definition of hematopoietic stem cells has changed in the last two decades. The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. HSCs constitute 1:10.000 of cells in myeloid tissue.HSCs are a heterogeneous population. The third category consists of the balanced (Bala) HSC, whose L/M ratio is between 3 and 10. Only the myeloid-biased and -balanced HSCs have durable self-renewal properties. In addition, serial transplantation experiments have shown that each subtype preferentially re-creates its blood cell type distribution, suggesting an inherited epigenetic program for each subtype.HSC studies through much of the past half century have led to a much deeper understanding. More recent advances have resulted in the use of HSC transplants in the treatment of cancers and other immune system disorders.