DNA Sequencing
... • RFLP detects DNA differences that affect restriction sites, because they “cut” differently if there are nucleotide variations • Restriction fragment analysis – Can rapidly provide useful comparative information about DNA sequences – Cut up the DNA region of interest using restriction enzymes, run ...
... • RFLP detects DNA differences that affect restriction sites, because they “cut” differently if there are nucleotide variations • Restriction fragment analysis – Can rapidly provide useful comparative information about DNA sequences – Cut up the DNA region of interest using restriction enzymes, run ...
What is Francisella? - Oregon State University
... Reported to be part of several countries biological warfare arsenal, including the United States • Aerosolization ...
... Reported to be part of several countries biological warfare arsenal, including the United States • Aerosolization ...
Molecular Basis of Heredity--ST03 1.2.7
... 6. Understand that specific genes regulate the functions performed by structures within the cells of multi-cellular organisms. Molecular Basis of Heredity--ST03 1.2.7 7. Describe how genetic information (DNA) in the cell is controlled at the molecular level and provides genetic continuity between ge ...
... 6. Understand that specific genes regulate the functions performed by structures within the cells of multi-cellular organisms. Molecular Basis of Heredity--ST03 1.2.7 7. Describe how genetic information (DNA) in the cell is controlled at the molecular level and provides genetic continuity between ge ...
Transformation
... incorporated into the recipient's chromosome (recombination). If the absorbed DNA is a plasmid, it will remain in the cytoplasm. When this DNA uptake occurs, the donor's genes are expressed, and donor polypeptides are synthesized. Transformation was discovered in 1928 by the British scientist Freder ...
... incorporated into the recipient's chromosome (recombination). If the absorbed DNA is a plasmid, it will remain in the cytoplasm. When this DNA uptake occurs, the donor's genes are expressed, and donor polypeptides are synthesized. Transformation was discovered in 1928 by the British scientist Freder ...
C2005/F2401 Key to Exam #3
... A & B (not required) – If the strep resistance gene works, then it was not cut up by Hind III, and nothing was inserted into it. It is intact. The pME plasmid had one Hind III site. When it was cut and the fragment inserted, each end of the fragment joined one half of the original Hind III site, mak ...
... A & B (not required) – If the strep resistance gene works, then it was not cut up by Hind III, and nothing was inserted into it. It is intact. The pME plasmid had one Hind III site. When it was cut and the fragment inserted, each end of the fragment joined one half of the original Hind III site, mak ...
Genetic backgrounds of each Escherichia coli strain used
... F-: This strain does not carry the F plasmid (DNA plasmid called Fertility Factor or Sex Factor). endA1: This strain lacks Endonuclease I (non-specific digestion) for cleaner preparations of DNA and better results in downstream applications. glnV44: In this strain a suppression of amber (UAG) stop c ...
... F-: This strain does not carry the F plasmid (DNA plasmid called Fertility Factor or Sex Factor). endA1: This strain lacks Endonuclease I (non-specific digestion) for cleaner preparations of DNA and better results in downstream applications. glnV44: In this strain a suppression of amber (UAG) stop c ...
12_PPTLecture_LEC
... 12.5 Reverse transcriptase helps make genes for cloning • Reverse transcriptase can be used to make smaller, complementary DNA (cDNA) libraries ...
... 12.5 Reverse transcriptase helps make genes for cloning • Reverse transcriptase can be used to make smaller, complementary DNA (cDNA) libraries ...
Characterization and determination of holin protein of Streptococcus suis bacteriophage SMP
... Background: Holins are a group of phage-encoded membrane proteins that control access of phage-encoded endolysins to the peptidoglycan, and thereby trigger the lysis process at a precise time point as the ‘lysis clock’. SMP is an isolated and characterized Streptococcus suis lytic phage. The aims of ...
... Background: Holins are a group of phage-encoded membrane proteins that control access of phage-encoded endolysins to the peptidoglycan, and thereby trigger the lysis process at a precise time point as the ‘lysis clock’. SMP is an isolated and characterized Streptococcus suis lytic phage. The aims of ...
Name - the BIOTECH Project
... 3. In the meantime, each group should get one LB agar plate and one LB agar + ampicillin plate. You will be plating bacteria with DNA on an LB agar plate and on an LB agar +ampicillian pate. Mark these two plates with the DNA number on your tube, your group members initials and class period. Also la ...
... 3. In the meantime, each group should get one LB agar plate and one LB agar + ampicillin plate. You will be plating bacteria with DNA on an LB agar plate and on an LB agar +ampicillian pate. Mark these two plates with the DNA number on your tube, your group members initials and class period. Also la ...
Experiment 2 Plasmid DNA Isolation, Restriction Digestion and Gel
... potassium has an additional effect on the sample. Potassium ions interact with the SDS making the detergent insoluble. The SDS will easily precipitate and can be separated by centrifugation. In doing so the insoluble SDS traps the larger genomic DNA and removes it from the supernatant. This leaves t ...
... potassium has an additional effect on the sample. Potassium ions interact with the SDS making the detergent insoluble. The SDS will easily precipitate and can be separated by centrifugation. In doing so the insoluble SDS traps the larger genomic DNA and removes it from the supernatant. This leaves t ...
PCR Clean-up Kit / 96-well PCR Clean
... 96-well V-shaped sample Plate onto the 96-well 1.6 ml growblock. Reposition the manifold top and place the Plasmid Plate on the manifold. Note: Be sure that the drip directors of the 96-well Plasmid Plate are aligned directly over the wells of the 96well V-shaped sample Plate. ...
... 96-well V-shaped sample Plate onto the 96-well 1.6 ml growblock. Reposition the manifold top and place the Plasmid Plate on the manifold. Note: Be sure that the drip directors of the 96-well Plasmid Plate are aligned directly over the wells of the 96well V-shaped sample Plate. ...
Chapter 11 : BIOTECHNOLOGY-PRINCIPLES
... 1. Genetic engineering : Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism. 2. Maintenance of sterile (microbial contamination-free) ambience in chemical engineering processes to enable growt ...
... 1. Genetic engineering : Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism. 2. Maintenance of sterile (microbial contamination-free) ambience in chemical engineering processes to enable growt ...
Chapter 27 Presentation
... Eukaryotic chromosomes have about 1000x as much DNA as do prokaryotes. Prokaryotes’ chromosomes are circular vs. linear; there is hardly any protein associated with them. Prokaryotic DNA is found in the nucleoid region, eukaryotic in the nucleus. Prokaryotic DNA is often associated with plasmids ...
... Eukaryotic chromosomes have about 1000x as much DNA as do prokaryotes. Prokaryotes’ chromosomes are circular vs. linear; there is hardly any protein associated with them. Prokaryotic DNA is found in the nucleoid region, eukaryotic in the nucleus. Prokaryotic DNA is often associated with plasmids ...
Prokaryote -( Wikipedia,)
... then the cell divides in two. However, they show a variety of parasexual processes where DNA is transferred between cells, such as transformation and transduction. ...
... then the cell divides in two. However, they show a variety of parasexual processes where DNA is transferred between cells, such as transformation and transduction. ...
Characterization of the Plasmid-Encoded Arsenic Salts Resistance
... oxytoca D12 into the unique EcoRI site of vector pUC18, forming pAE48 [5, 6]. When plasmid pAE48 was transformed into the E. coli, transformant cells grew in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. However, cells without pAE48 failed to grow in the presence of 2 mM arsen ...
... oxytoca D12 into the unique EcoRI site of vector pUC18, forming pAE48 [5, 6]. When plasmid pAE48 was transformed into the E. coli, transformant cells grew in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. However, cells without pAE48 failed to grow in the presence of 2 mM arsen ...
CHAPTER 8 Recombinant DNA Technology
... Animation: DNA Cloning in a Plasmid Vector 1. Several types of cloning vectors have been constructed, each with different molecular properties and cloning capacity. 2. Plasmid cloning vectors are derived from natural plasmids, circles of dsDNA that include origin sequences (ori) needed for replicati ...
... Animation: DNA Cloning in a Plasmid Vector 1. Several types of cloning vectors have been constructed, each with different molecular properties and cloning capacity. 2. Plasmid cloning vectors are derived from natural plasmids, circles of dsDNA that include origin sequences (ori) needed for replicati ...
HPV DNA PARTITIONING DURING MITOSIS AS FOLLOWED
... The HPV genome can be divided into three regions: the upstream regulatory region (URR), the early (E) genes, and the late (L) genes. All open reading frames (ORFs) are encoded by the same DNA strand of the viral genome. All mRNA transcription proceeds in the same direction. Utilization of several pr ...
... The HPV genome can be divided into three regions: the upstream regulatory region (URR), the early (E) genes, and the late (L) genes. All open reading frames (ORFs) are encoded by the same DNA strand of the viral genome. All mRNA transcription proceeds in the same direction. Utilization of several pr ...
GcvA, a LysR-type transcriptional regulator protein
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
... was partially digested with Sau3A to give a random distribution of DNA fragments. Size-fractionated fragments of between 5 and 10 kb were ligated into vector pSU19, previously digested with BamHI and treated with calf intestinal alkaline phosphatase to prevent re-annealing. Recombinant DNA was intro ...
DNA RESTRICTION ANALYSIS
... into space marked with red line. There is a leveling bubble which can be used to level the gel casting tray (by turning knobs at bottom). 2. Place tray FLAT where agarose can be poured and allowed to set UNDISTURBED. 3. Carefully pour the entire contents of bottle (40ml of agarose solution) liquifie ...
... into space marked with red line. There is a leveling bubble which can be used to level the gel casting tray (by turning knobs at bottom). 2. Place tray FLAT where agarose can be poured and allowed to set UNDISTURBED. 3. Carefully pour the entire contents of bottle (40ml of agarose solution) liquifie ...
FAQs about experiments that are exempt from NIH Guidelines
... The deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the use of the drug to control disease agents in humans, veterinary medicine or agriculture [Section III-A]; ...
... The deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the use of the drug to control disease agents in humans, veterinary medicine or agriculture [Section III-A]; ...
Genetic Basis of Variation in Bacteria
... Organization of genetic material in bacteria: plasmids Examples of naturally-occuring plasmids and relevant features ...
... Organization of genetic material in bacteria: plasmids Examples of naturally-occuring plasmids and relevant features ...
molecular biology
... either the reaction volume has to be increased or an enzyme preparation with higher activity has to be used. ...
... either the reaction volume has to be increased or an enzyme preparation with higher activity has to be used. ...
Présentation PowerPoint
... Molecular mechanisms of antibiotics resistance, molecular mechanisms of transmission of resistance genes: ...
... Molecular mechanisms of antibiotics resistance, molecular mechanisms of transmission of resistance genes: ...
Transcriptional activity and role of plasmids of
... than 3 Log2 FC in mM+Hops, compared to only the top four SDE genes in Lb464 expressed at this level (Table 3). The difference in total SDE transcripts was unexpected given that hop extract was clearly affecting the growth rate of each bacterium (Figure 1). Although there are fewer genes SDE at level ...
... than 3 Log2 FC in mM+Hops, compared to only the top four SDE genes in Lb464 expressed at this level (Table 3). The difference in total SDE transcripts was unexpected given that hop extract was clearly affecting the growth rate of each bacterium (Figure 1). Although there are fewer genes SDE at level ...
DNA Sequencing
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
... Gel electrophoresis separates macromolecules on the basis of their rate of movement through a gel in an electric field. How far a DNA molecule travels while the current is on is inversely proportional to its length. A mixture of DNA molecules, usually fragments produced by restriction enzyme digesti ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.