
1. Description of the course
... immune-, radioprotective). Structure and function of the immune system. Immunocompetent cells. T-lymphocytes, ontogenesis. B-lymphocytes, ontogenesis. Subpopulation of B cells. Cooperation between immunocompetent cells in the immune response. Antigens as inducers of immune responses. Complete antige ...
... immune-, radioprotective). Structure and function of the immune system. Immunocompetent cells. T-lymphocytes, ontogenesis. B-lymphocytes, ontogenesis. Subpopulation of B cells. Cooperation between immunocompetent cells in the immune response. Antigens as inducers of immune responses. Complete antige ...
Obesity could be catching
... Obesity could be catching A new report into obesity shows that being overweight could be catching. Scientists have discovered an obesity virus that might be as easy to catch as a cold. American researchers say that washing your hands could be one way to kill the virus and avoid becoming overweight. ...
... Obesity could be catching A new report into obesity shows that being overweight could be catching. Scientists have discovered an obesity virus that might be as easy to catch as a cold. American researchers say that washing your hands could be one way to kill the virus and avoid becoming overweight. ...
Orr M.T., K.H. Edelmann, J. Vieira, L. Corey, D.H. Raulet, and C.B. Wilson. 2005. Inhibition of MHC class I is a virulence factor in herpes simplex virus infection of mice. PLoS Pathog 1(1):e7.
... recombination with KOS strain HSV-1. Contents and location of insertions are indicated. Arrows indicate direction of transcription. The probe used to isolate correctly recombined viruses is indicated. (B) Genomic DNA from KOS, 27US11, 27m152, 27gfp, and 27gfpR was digested with the indicated restric ...
... recombination with KOS strain HSV-1. Contents and location of insertions are indicated. Arrows indicate direction of transcription. The probe used to isolate correctly recombined viruses is indicated. (B) Genomic DNA from KOS, 27US11, 27m152, 27gfp, and 27gfpR was digested with the indicated restric ...
RNA genomes
... Virology breakthrough in the 1950’s: The viral nucleic acid genome is the genetic code ...
... Virology breakthrough in the 1950’s: The viral nucleic acid genome is the genetic code ...
SIMPLE SEROLOGICAL TECHNIQUES
... (1) An example of this is the administration of diphtheria antitoxin made from horse serum, which is a foreign protein to humans. (2) People given too much antitoxin (i.e., antigen) may develop serum sickness. b. The soluble complexes are not handled well by the reticuloendothelial system (RES). 3. ...
... (1) An example of this is the administration of diphtheria antitoxin made from horse serum, which is a foreign protein to humans. (2) People given too much antitoxin (i.e., antigen) may develop serum sickness. b. The soluble complexes are not handled well by the reticuloendothelial system (RES). 3. ...
Formation of Clear Zones with
... ‘ scatter zones ’ to distinguish them from the differently formed ‘ghost zones ’ of the sensitive organism. In large zones, 10 mm. or more in width, the edge was sharp and sufficiently well demarcated to be measured to within 0.1 mm. with accuracy, but it was finely and irregularly crenated, so that ...
... ‘ scatter zones ’ to distinguish them from the differently formed ‘ghost zones ’ of the sensitive organism. In large zones, 10 mm. or more in width, the edge was sharp and sufficiently well demarcated to be measured to within 0.1 mm. with accuracy, but it was finely and irregularly crenated, so that ...
Table of Contents
... reporting laboratory experiments and writing research papers. Over the years, I have therefore, used my teaching time to encourage students to write. In order to encourage students to be enthusiastic about writing, I have adopted a non-punitive attitude in which drafts are given feedback and only th ...
... reporting laboratory experiments and writing research papers. Over the years, I have therefore, used my teaching time to encourage students to write. In order to encourage students to be enthusiastic about writing, I have adopted a non-punitive attitude in which drafts are given feedback and only th ...
(2010). Tobacco mild green mosaic virus in Impatiens and
... Although the virus has been reported on different hosts in different countries it is assumed that the virus could be associated with any of the known host plants in the countries where it is known to occur. The virus may occur in other countries (countries from which interceptions have occurred but ...
... Although the virus has been reported on different hosts in different countries it is assumed that the virus could be associated with any of the known host plants in the countries where it is known to occur. The virus may occur in other countries (countries from which interceptions have occurred but ...
RNA viruses in the sea - Associação Brasileira de Medicina
... Fig. 2. Epifluorescence microscopy image of a seawater sample stained with the nucleic acid dye YO-PRO-1 as described (Wen et al., 2004). The larger particles are prokaryotes and the smaller particles are viruses/viruslike particles (VLPs). The estimate of VLPs by this method in this coastal sample ...
... Fig. 2. Epifluorescence microscopy image of a seawater sample stained with the nucleic acid dye YO-PRO-1 as described (Wen et al., 2004). The larger particles are prokaryotes and the smaller particles are viruses/viruslike particles (VLPs). The estimate of VLPs by this method in this coastal sample ...
RNA viruses in the sea - SOEST
... microorganisms. The overall effect of this viral activity is to augment the rate of movement of nutrients from particulate organic matter to dissolved organic matter, diverting nutrients from higher trophic levels back into the microbial fraction (Fuhrman, 1992; Suttle, 2007). Viruses also appear to ...
... microorganisms. The overall effect of this viral activity is to augment the rate of movement of nutrients from particulate organic matter to dissolved organic matter, diverting nutrients from higher trophic levels back into the microbial fraction (Fuhrman, 1992; Suttle, 2007). Viruses also appear to ...
No Slide Title - Bruce Blumberg
... gene expression – if not, most any selection will work • what is already working in the lab or surrounding labs? – Are there time constraints that must be addressed? – Short term vs long term goals • will the cell type require multiple rounds of selection? • Are there enough selective markers availa ...
... gene expression – if not, most any selection will work • what is already working in the lab or surrounding labs? – Are there time constraints that must be addressed? – Short term vs long term goals • will the cell type require multiple rounds of selection? • Are there enough selective markers availa ...
SWARTZ MICROBIOLOGY NOTES
... technique to isolate special kinds of microorganisms from soil and water. He also discovered the bacteria that grow in the root nodules of legumes such as alfalfa, clover and soybean can fix nitrogen. In 1928, Alexander Fleming (1881-1955) discovered penicillin (produced by Penicillium notatum). The ...
... technique to isolate special kinds of microorganisms from soil and water. He also discovered the bacteria that grow in the root nodules of legumes such as alfalfa, clover and soybean can fix nitrogen. In 1928, Alexander Fleming (1881-1955) discovered penicillin (produced by Penicillium notatum). The ...
Microbiology of Periodontal Diseases
... interaction, replacing Gram (+) cocci and rods Emergence of Gram (-) filamentous bacteria Matrix of microorganisms and a ground substance ...
... interaction, replacing Gram (+) cocci and rods Emergence of Gram (-) filamentous bacteria Matrix of microorganisms and a ground substance ...
SYLVIA ELLINGER, RUDI GLOCKSHUBER, GERHARD JAHN, 1
... the outer env glycoprotein gp120 and its precursor gp160. Sera from a number of HIV -infected individuals may have an indeterminate pattern because of a loss of antibodies to non-env proteins in advanced immunodeficiency (20) or a lack of detectable env-specific antibodies, as described for the earl ...
... the outer env glycoprotein gp120 and its precursor gp160. Sera from a number of HIV -infected individuals may have an indeterminate pattern because of a loss of antibodies to non-env proteins in advanced immunodeficiency (20) or a lack of detectable env-specific antibodies, as described for the earl ...
Escherichia coli
... implications for cost effective uses in the clinical setting. Escherichia coli biofilm formation. ...
... implications for cost effective uses in the clinical setting. Escherichia coli biofilm formation. ...
S - College of Veterinary Medicine
... After-hours submissions to the ISU Vet Diagnostic Lab can now be placed directly in a refrigerator located inside the VDL submission door foyer. After entering the foyer, follow the procedure below for unlocking the refrigerator and leaving samples for submission: • Paperwork MUST accompany the subm ...
... After-hours submissions to the ISU Vet Diagnostic Lab can now be placed directly in a refrigerator located inside the VDL submission door foyer. After entering the foyer, follow the procedure below for unlocking the refrigerator and leaving samples for submission: • Paperwork MUST accompany the subm ...
PPT - Bruce Blumberg
... recombination – if not, most any selection will work • what is already working in the lab or surrounding labs? – Are there time constraints that must be addressed? – Short term vs long term goals • will the cell type require multiple rounds of selection? • Are there enough selective markers availabl ...
... recombination – if not, most any selection will work • what is already working in the lab or surrounding labs? – Are there time constraints that must be addressed? – Short term vs long term goals • will the cell type require multiple rounds of selection? • Are there enough selective markers availabl ...
World Hepatitis Day 28th July, 2016
... July 28, annually is celebrated as World Hepatitis Day, established by the World Health Organization, aims to increase awareness about hepatitis. It provides an opportunity to focus on specific actions such as strengthening prevention, screening and control of hepatitis. Around 400 million people ar ...
... July 28, annually is celebrated as World Hepatitis Day, established by the World Health Organization, aims to increase awareness about hepatitis. It provides an opportunity to focus on specific actions such as strengthening prevention, screening and control of hepatitis. Around 400 million people ar ...
Microbial Detection and Elimination
... sensitive detection of up to 40 mycoplasma and acholeplasma species, including all species known to contaminate cell cultures. The test can be performed within 3 hours and includes Internal Control DNA (as negative control and for verifying a successful PCR run) as well as Positive Control DNA. All ...
... sensitive detection of up to 40 mycoplasma and acholeplasma species, including all species known to contaminate cell cultures. The test can be performed within 3 hours and includes Internal Control DNA (as negative control and for verifying a successful PCR run) as well as Positive Control DNA. All ...
Teacher`s Guide
... 2. Depending on time, consider assigning students three or four diseases to research, or allow them to choose some that interest them. 3. Students add the information about diseases to their T-charts. Teacher note: You may need to guide students on information-gathering and organization strategies. ...
... 2. Depending on time, consider assigning students three or four diseases to research, or allow them to choose some that interest them. 3. Students add the information about diseases to their T-charts. Teacher note: You may need to guide students on information-gathering and organization strategies. ...
Principles of Virology
... • Essential for all viruses except those of yeasts (no extracellular phases) and plants (enter cells by mechanical damage) • Receptors and co-receptors • Different virions can bind the same receptor Adenovirus and Coxsackievirus B3 have a common primary receptor: The swine herpesvirus binds the same ...
... • Essential for all viruses except those of yeasts (no extracellular phases) and plants (enter cells by mechanical damage) • Receptors and co-receptors • Different virions can bind the same receptor Adenovirus and Coxsackievirus B3 have a common primary receptor: The swine herpesvirus binds the same ...
Chapter 1 Microbes by Design 1
... 5. What is important about the species of bacteria called Rhizobium leguminosarum? a. They are denitrifying bacteria that add in the decomposition of organic matter. b. They are nitrogen-fixing bacteria that add nourishment through root nodules. c. They are nitrifying bacteria that release ammonia. ...
... 5. What is important about the species of bacteria called Rhizobium leguminosarum? a. They are denitrifying bacteria that add in the decomposition of organic matter. b. They are nitrogen-fixing bacteria that add nourishment through root nodules. c. They are nitrifying bacteria that release ammonia. ...
Chapter 13 Evolution and human health
... a genome of 8 RNA strands that code for a total of 10 proteins. ...
... a genome of 8 RNA strands that code for a total of 10 proteins. ...
The effect of histo-blood group antigen (HBGA)
... co-incubated with cell-free bacteria culture filtrate for 48 h at 37°C: a 2.4 log reduction in plaque forming units per mL (PFU/mL). A reduction greater than 5 log PFU/mL was observed when the virus was co-incubated with a high number of bacterial cells in a skim milk-sucrose matrix. However, it is ...
... co-incubated with cell-free bacteria culture filtrate for 48 h at 37°C: a 2.4 log reduction in plaque forming units per mL (PFU/mL). A reduction greater than 5 log PFU/mL was observed when the virus was co-incubated with a high number of bacterial cells in a skim milk-sucrose matrix. However, it is ...
4 The body at war
... They consist of DNA or RNA enclosed within one or more protein coats. Viruses are so small that they can only be seen with very powerful electron microscopes. Scientists debate whether viruses should be called living things as they are obligate intracellular parasites. This means that they need to i ...
... They consist of DNA or RNA enclosed within one or more protein coats. Viruses are so small that they can only be seen with very powerful electron microscopes. Scientists debate whether viruses should be called living things as they are obligate intracellular parasites. This means that they need to i ...
Virus quantification

Virus quantification involves counting the number of viruses in a specific volume to determine the virus concentration. It is utilized in both research and development (R&D) in commercial and academic laboratories as well as production situations where the quantity of virus at various steps is an important variable. For example, the production of viral vaccines, recombinant proteins using viral vectors and viral antigens all require virus quantification to continually adapt and monitor the process in order to optimize production yields and respond to ever changing demands and applications. Examples of specific instances where known viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization and adaptation of methods to cell culture. This page discusses various techniques currently used to quantify viruses in liquid samples. These methods are separated into two categories, traditional vs. modern methods. Traditional methods are industry-standard methods that have been used for decades but are generally slow and labor-intensive. Modern methods are relatively new commercially available products and kits that greatly reduce quantification time. This is not meant to be an exhaustive review of all potential methods, but rather a representative cross-section of traditional methods and new, commercially available methods. While other published methods may exist for virus quantification, non-commercial methods are not discussed here.