Protein engineering: navigating between chance and reason
... potential protein sequences. With so many possibilities, protein engineers are hunting for more efficient ways to explore sequence space. Whereas cell-free systems can be used to churn through greater numbers of proteins (including proteins toxic to yeast and bacteria), cell-based systems offer a re ...
... potential protein sequences. With so many possibilities, protein engineers are hunting for more efficient ways to explore sequence space. Whereas cell-free systems can be used to churn through greater numbers of proteins (including proteins toxic to yeast and bacteria), cell-based systems offer a re ...
Enzymes: Regulation 2-3
... allosteric regulation: an immediate and localized response, so rapid activity changes covalent modifications: slower and longer-lasting effects with coordinated systemic effects (e.g., a single hormone can trigger covalent modification events that change activities of metabolic enzymes in a many tis ...
... allosteric regulation: an immediate and localized response, so rapid activity changes covalent modifications: slower and longer-lasting effects with coordinated systemic effects (e.g., a single hormone can trigger covalent modification events that change activities of metabolic enzymes in a many tis ...
Endoplasmic Reticulum-Localized Amyloid β
... Budding of transport vesicles from the microsomes was unlikely under the experimental conditions used here. Nevertheless, the possibility that the Ab42 in the supernatant was contained within transport vesicles and not translocated across the microsomal membrane was analyzed by protease protection a ...
... Budding of transport vesicles from the microsomes was unlikely under the experimental conditions used here. Nevertheless, the possibility that the Ab42 in the supernatant was contained within transport vesicles and not translocated across the microsomal membrane was analyzed by protease protection a ...
Extracellular Macromolecules
... endothelial surface sialidases slowly remove sialates from these circulating proteins rate of sialate removal depends on protein's structure ...
... endothelial surface sialidases slowly remove sialates from these circulating proteins rate of sialate removal depends on protein's structure ...
Escherichia coli
... Due to hydrophobic and amphiphilic nature Less than 1% of high resolution 3D structures known ...
... Due to hydrophobic and amphiphilic nature Less than 1% of high resolution 3D structures known ...
Fluorescent proteins Green Fluorescence Protein
... biological science by providing a way to monitor how individual genes are regulated and expressed within a living cell ; Localization and tracing of a target protein • Widespread use by their expression in other organisms as a reporter • Usually fused to N- or C-terminus of proteins by gene manipula ...
... biological science by providing a way to monitor how individual genes are regulated and expressed within a living cell ; Localization and tracing of a target protein • Widespread use by their expression in other organisms as a reporter • Usually fused to N- or C-terminus of proteins by gene manipula ...
Catalog# 786-842 PROTOCOL - G
... of proteins. The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds. The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants. Heparin is a linear glycosaminoglycan composed of equimola ...
... of proteins. The resin consists of 6% cross-linked agarose covalently coupled to heparin through amide bonds. The coupling chemistry used generates a highly stable purification resin that is stable most commonly used buffers and denaturants. Heparin is a linear glycosaminoglycan composed of equimola ...
Protein Labeling
... being completely genetically encodable. However, the large size of GFP (25 kDa) can ...
... being completely genetically encodable. However, the large size of GFP (25 kDa) can ...
Protein–protein interactions
... than previously thought. In 2000 the number was estimated at perhaps 50 000–90 000. The current figure is a little over 20 000. How can organisms so complicated be constructed from so few ‘instructions’? The answer seems in part to be that it’s not so much about how many genes you have, but how you ...
... than previously thought. In 2000 the number was estimated at perhaps 50 000–90 000. The current figure is a little over 20 000. How can organisms so complicated be constructed from so few ‘instructions’? The answer seems in part to be that it’s not so much about how many genes you have, but how you ...
methods to visualize newly synthesized proteins in situ
... Following protein lifetime, distribution changes and synthesis rate changes with FUNCAT-PLA ...
... Following protein lifetime, distribution changes and synthesis rate changes with FUNCAT-PLA ...
Modeling dynamics of cell-to-cell variability in TRAIL
... each of the 104 (105 for results presented in main text) mother cells. This duration was verified by comparison with analytical results to be sufficient to reach the steady-state distribution. Sister cells were simply constructed by duplication of the mother cell state. Because in experiments from [ ...
... each of the 104 (105 for results presented in main text) mother cells. This duration was verified by comparison with analytical results to be sufficient to reach the steady-state distribution. Sister cells were simply constructed by duplication of the mother cell state. Because in experiments from [ ...
Cytoskeleton Handout
... The mitotic cycle (i.e., cell division) involves the disassembly of the cytoplasmic microtubules and the assembly of the spindle apparatus. At least 4 kinesins are involved in this process. Possible roles include: 1) stabilizing the metaphase plane, 2) sliding the spindle microtubules in opposite di ...
... The mitotic cycle (i.e., cell division) involves the disassembly of the cytoplasmic microtubules and the assembly of the spindle apparatus. At least 4 kinesins are involved in this process. Possible roles include: 1) stabilizing the metaphase plane, 2) sliding the spindle microtubules in opposite di ...
Post-transcriptional gene control
... • Group I self-splicing introns occur in rRNA genes of protozoans • Group II self-splicing introns occur in chloroplasts and mitochondria of plants and ...
... • Group I self-splicing introns occur in rRNA genes of protozoans • Group II self-splicing introns occur in chloroplasts and mitochondria of plants and ...
Structural studies of phosphoinositide 3-kinase
... In yeast, these steps are also dependent on the 18 class E Vps proteins [1]. In mammalian cells, a more complex picture is emerging which suggests that the formation of internal vesicles does not require all of the class E homologues [2,3]. PtdIns3P is abundant on endosomal membranes and the interna ...
... In yeast, these steps are also dependent on the 18 class E Vps proteins [1]. In mammalian cells, a more complex picture is emerging which suggests that the formation of internal vesicles does not require all of the class E homologues [2,3]. PtdIns3P is abundant on endosomal membranes and the interna ...
Proteomics investigation into cardiac endothelial
... endothelial cells (CMECs). • CMECs show distinct structural and functional adaptations compared to other endothelial cell phenotypes in view of their location in the myocardium where they are closely associated with surrounding cardiomyocytes. • There is intimate CMEC-cardiomyocyte arrangement • car ...
... endothelial cells (CMECs). • CMECs show distinct structural and functional adaptations compared to other endothelial cell phenotypes in view of their location in the myocardium where they are closely associated with surrounding cardiomyocytes. • There is intimate CMEC-cardiomyocyte arrangement • car ...
doc - Gogarten Lab
... are 20 possible amino acids? For your answer only consider the principles of combinatorics and ignore possible incompatibilities between amino acids) (1pt) ...
... are 20 possible amino acids? For your answer only consider the principles of combinatorics and ignore possible incompatibilities between amino acids) (1pt) ...
Ms. Robyn Klemptner
... Avrova, A.O., Taleb, N., Rokka, V-M., Heilbronn, J., Campbell, E., Hein, I., Gilroy, E.M., Cardle, L., Bradshaw, J.E., Stewart, H.E., Fakim, Y.J., Loake, G. and Birch, P.R.J. (2004) Potato oxysterol binding protein and cathepsin B are rapidly up-regulated in independent defense pathways that disting ...
... Avrova, A.O., Taleb, N., Rokka, V-M., Heilbronn, J., Campbell, E., Hein, I., Gilroy, E.M., Cardle, L., Bradshaw, J.E., Stewart, H.E., Fakim, Y.J., Loake, G. and Birch, P.R.J. (2004) Potato oxysterol binding protein and cathepsin B are rapidly up-regulated in independent defense pathways that disting ...
Mechanisms of Hormonal Action
... Glucagon and epinephrine bind to different receptors yet activate the same G protein which in turn activates the same effector protein, adenylate kinase. Other effector proteins activated by other G proteins are phospholipase C, phospholipase A2, potassium channels, sodium channels, calcium channels ...
... Glucagon and epinephrine bind to different receptors yet activate the same G protein which in turn activates the same effector protein, adenylate kinase. Other effector proteins activated by other G proteins are phospholipase C, phospholipase A2, potassium channels, sodium channels, calcium channels ...
clarisoy™ protein made clear
... protein that offers both clarity and complete protein nutrition for beverages with a pH of less than 4. • CLARISOY™ 150 is a revolutionary soy protein for neutral pH beverages and low pH beverage with cloud systems. The clean flavor and smooth mouth-feel pairs perfectly with flavors like vanilla, s ...
... protein that offers both clarity and complete protein nutrition for beverages with a pH of less than 4. • CLARISOY™ 150 is a revolutionary soy protein for neutral pH beverages and low pH beverage with cloud systems. The clean flavor and smooth mouth-feel pairs perfectly with flavors like vanilla, s ...
Handbook of Protein Sequences: A Compilation of Amino Acid
... The Handbook produced by Dr. Croft is a testimony to his industry and patience. It should find a place in a11 biochemistry departments. While appreciative of the difficulties involved and bearing in mind the intention to supply annual updates, one must comment that the lay-out might have been improv ...
... The Handbook produced by Dr. Croft is a testimony to his industry and patience. It should find a place in a11 biochemistry departments. While appreciative of the difficulties involved and bearing in mind the intention to supply annual updates, one must comment that the lay-out might have been improv ...
PROTEIN STRUCTURE SIMILARITY CALCULATION AND VISUALIZATION
... The all by all pairwise similarity calculated in moduleA will be used as input to moduleB. Output should be connectivity graph (as shown in next slide) between all proteins. Each edge must display the similarity value. Preferred output will be each edge length weighted as similarity value between th ...
... The all by all pairwise similarity calculated in moduleA will be used as input to moduleB. Output should be connectivity graph (as shown in next slide) between all proteins. Each edge must display the similarity value. Preferred output will be each edge length weighted as similarity value between th ...
Recombinant Ebola virus VP40 matrix protein
... Ebola virus (EBOV) matrix protein (VP40) is expressed in E. coli and the recombinant protein purified using FPLC. Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles. Size: 100 µg of protein is supplied in HEPES buffer pH ...
... Ebola virus (EBOV) matrix protein (VP40) is expressed in E. coli and the recombinant protein purified using FPLC. Storage: -80°C. It is recommended to dispense single-use aliquots and store aliquots at -80°C to avoid multiple freeze/thaw cycles. Size: 100 µg of protein is supplied in HEPES buffer pH ...
Mitochondrial quality control by the ubiquitin
... The UPS (ubiquitin–proteasome system) and ERAD [ER (endoplasmic reticulum)associated degradation] Because the OMM (outer mitochondrial membrane) is contiguous with the cytosol, it may be accessed by cytosolic proteolytic systems, such as the UPS [10–12]. Most targeted cellular protein degradation is ...
... The UPS (ubiquitin–proteasome system) and ERAD [ER (endoplasmic reticulum)associated degradation] Because the OMM (outer mitochondrial membrane) is contiguous with the cytosol, it may be accessed by cytosolic proteolytic systems, such as the UPS [10–12]. Most targeted cellular protein degradation is ...
Transient intracellular expression of chicken UCH-L3 and
... if they could be established at greater than 95.0% probability by the Peptide Prophet algorithm with Scaffold delta-mass correction. Protein identifications were accepted if they could be established at greater than 99.0% probability and contained at least 1 identified peptide. Protein probabilities ...
... if they could be established at greater than 95.0% probability by the Peptide Prophet algorithm with Scaffold delta-mass correction. Protein identifications were accepted if they could be established at greater than 99.0% probability and contained at least 1 identified peptide. Protein probabilities ...
Proteasome
Proteasomes are protein complexes inside all eukaryotes and archaea, and in some bacteria. The main function of the proteasome is to degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds.