1 Evolutionary conservation and emerging functional diversity of the
... stimulate their otherwise weak intrinsic ATPase activity. ATP hydrolysis results in profound conformational changes in the client binding domain (CBD), thereby modulating substrate executions and thus driving Hsp70’s functions (Kampinga and Craig 2010). In this way, Hsp70s, along with their obligate ...
... stimulate their otherwise weak intrinsic ATPase activity. ATP hydrolysis results in profound conformational changes in the client binding domain (CBD), thereby modulating substrate executions and thus driving Hsp70’s functions (Kampinga and Craig 2010). In this way, Hsp70s, along with their obligate ...
Full Text
... understanding of interactions in protein structures. Therefore, we have been interested in developing a representation for biological sequences that can incorporate structural features conferred through dependences among amino acids. We have used Bayesian networks (Neapolitan, 1990; Pearl, 1988) to ...
... understanding of interactions in protein structures. Therefore, we have been interested in developing a representation for biological sequences that can incorporate structural features conferred through dependences among amino acids. We have used Bayesian networks (Neapolitan, 1990; Pearl, 1988) to ...
Comparison of cell-surface L-amino acid oxidases
... When certain L-amino acids were added to preconditioned AMPHID, PRYM, or COCCOII cultures, H 2 0 2 , NH4+, and an a-keto acid were produced d u e to the presence of a cell-surface L-amino acid oxidase (Palenik & Morel 1990). For example, when 1 yM Lalanine was added to a nitrogen-limited culture of ...
... When certain L-amino acids were added to preconditioned AMPHID, PRYM, or COCCOII cultures, H 2 0 2 , NH4+, and an a-keto acid were produced d u e to the presence of a cell-surface L-amino acid oxidase (Palenik & Morel 1990). For example, when 1 yM Lalanine was added to a nitrogen-limited culture of ...
Thermostability of the human respiratory syncytial virus fusion
... FD1–109 and F2* intermediates were cleaved at the remaining furin sites, whereas the F1 polypeptide remained essentially unchanged, although low levels of new bands moving faster than F1 were also observed (Fig. 1b). To test the effect of trypsin on the aggregated state of FTM{ and D137–146, the two ...
... FD1–109 and F2* intermediates were cleaved at the remaining furin sites, whereas the F1 polypeptide remained essentially unchanged, although low levels of new bands moving faster than F1 were also observed (Fig. 1b). To test the effect of trypsin on the aggregated state of FTM{ and D137–146, the two ...
CONVERSATIONS ON CHELATION AND MINERAL NUTRITION
... How does one evaluate chelates against the other mineral forms in the marketplace which also talk of availability? Simply ask the following questions: 1) Does the product have a patent number? 2) What is the chelating agent in the product and what concentration is the chelate? 3) Are the minerals tr ...
... How does one evaluate chelates against the other mineral forms in the marketplace which also talk of availability? Simply ask the following questions: 1) Does the product have a patent number? 2) What is the chelating agent in the product and what concentration is the chelate? 3) Are the minerals tr ...
molecular biology
... Like the synthesis of DNA (replication) and RNA (transcription), specific cellular machinery directs protein synthesis (translation). While differences exist, one can also see some common pattern directing the biological synthesis of these polymers viz., proteins, RNA and DNA. The syntheses of all t ...
... Like the synthesis of DNA (replication) and RNA (transcription), specific cellular machinery directs protein synthesis (translation). While differences exist, one can also see some common pattern directing the biological synthesis of these polymers viz., proteins, RNA and DNA. The syntheses of all t ...
Functional Control by Codon Bias in Magnetic Bacteria
... similar blast searches for Iron Regulatory Proteins (IRPs: IRP1 and IRP2) from mammalian18–20 as well as bacterial sources (in bacteria, IRP1 is called aconitase) in the magnetic bacterial genome. The logic behind these searches was that if IRPs are present in magnetic bacteria, then one would expec ...
... similar blast searches for Iron Regulatory Proteins (IRPs: IRP1 and IRP2) from mammalian18–20 as well as bacterial sources (in bacteria, IRP1 is called aconitase) in the magnetic bacterial genome. The logic behind these searches was that if IRPs are present in magnetic bacteria, then one would expec ...
Lesson 2 - The George S. Wise Faculty of Life Sciences
... Counted the number of the substitutions per amino acid pair (20 x 20) Found that common substitutions occurred between chemically similar amino acids ...
... Counted the number of the substitutions per amino acid pair (20 x 20) Found that common substitutions occurred between chemically similar amino acids ...
Design and application of stimulus
... The ability of peptides and proteins to change conformations in response to external stimuli such as temperature, pH and the presence of specific small molecules is ubiquitous in nature. Exploiting this phenomenon, numerous natural and designed peptides have been used to engineer stimulus-responsive ...
... The ability of peptides and proteins to change conformations in response to external stimuli such as temperature, pH and the presence of specific small molecules is ubiquitous in nature. Exploiting this phenomenon, numerous natural and designed peptides have been used to engineer stimulus-responsive ...
Lecture 8
... • For looking at proteins without charge, proteins can be treated with 6M urea (denatures but unlike SDS does not put charges on a protein). • Thus, a mixture of proteins can be electrophoresed through a solution having a a stable pH gradient in from the anode to the cathode and a each protein will ...
... • For looking at proteins without charge, proteins can be treated with 6M urea (denatures but unlike SDS does not put charges on a protein). • Thus, a mixture of proteins can be electrophoresed through a solution having a a stable pH gradient in from the anode to the cathode and a each protein will ...
NMR spectroscopy brings invisible protein states into
... exchange process can be obtained from obtained from which details of the exchange reaction can be obtained. this probe, although information may be available from the PRE effect (Box 2). If the spin exists in a single state for time T and a ‘refocusing’ pulse (Fig. 1b,c, black bars) is applied exact ...
... exchange process can be obtained from obtained from which details of the exchange reaction can be obtained. this probe, although information may be available from the PRE effect (Box 2). If the spin exists in a single state for time T and a ‘refocusing’ pulse (Fig. 1b,c, black bars) is applied exact ...
No Slide Title
... a 16-kD position (31), where it now co-migrated with the unmodified S monomer, Sp16, from crude brain extracts (Fig. 5C). We conclude from our data obtained by mass spectrometry analysis (21) and these enzymatic digestions that Sp22 is a posttranslationally modified form of human Sp16 containing O-l ...
... a 16-kD position (31), where it now co-migrated with the unmodified S monomer, Sp16, from crude brain extracts (Fig. 5C). We conclude from our data obtained by mass spectrometry analysis (21) and these enzymatic digestions that Sp22 is a posttranslationally modified form of human Sp16 containing O-l ...
Immunodetection of the expression of microsomal proteins encoded
... site and transporters for the entry of the substrate G-6-P (glucose 6-phosphate) and for the exit of the products, phosphate and glucose [4–7]. The genetic deficiency of the G-6-Pase1 enzyme protein is termed type 1a glycogen storage disease (GSD1a) [2,8]. However, it has been known for a long time ...
... site and transporters for the entry of the substrate G-6-P (glucose 6-phosphate) and for the exit of the products, phosphate and glucose [4–7]. The genetic deficiency of the G-6-Pase1 enzyme protein is termed type 1a glycogen storage disease (GSD1a) [2,8]. However, it has been known for a long time ...
PDF Format - Kinexus Bioinformatics Corporation
... Our proteomics and bioinformatics services platform enables the discovery of kinase drug targets and characterization of the actions of drug leads for our pharmaceutical clients. As illustrated in this figure, most steps in the drug discovery and development pipeline can benefit from our diverse se ...
... Our proteomics and bioinformatics services platform enables the discovery of kinase drug targets and characterization of the actions of drug leads for our pharmaceutical clients. As illustrated in this figure, most steps in the drug discovery and development pipeline can benefit from our diverse se ...
Summary Affimer reagents in affinity resins Specific target binding
... repeated injection and elution of pure hIgG (81 runs), including 19 clean-inplace (CIP) cycles (100 mM NaOH, 10 min). The data indicate consistent capture performance over the course of this study (Figure 4A). No leaching from Affimer columns was detected by western blotting of concentrated elution ...
... repeated injection and elution of pure hIgG (81 runs), including 19 clean-inplace (CIP) cycles (100 mM NaOH, 10 min). The data indicate consistent capture performance over the course of this study (Figure 4A). No leaching from Affimer columns was detected by western blotting of concentrated elution ...
Hydrogen exchange mass spectrometry for the analysis of protein
... content of 99% or more. Dilutions of 15-fold or greater will produce final deuterium concentrations of >95%. This serves to force the labeling reaction (k2) in one direction (see Eq. 3). However, with this labeling method the original protein sample is diluted. Such a dilution may not be compatible ...
... content of 99% or more. Dilutions of 15-fold or greater will produce final deuterium concentrations of >95%. This serves to force the labeling reaction (k2) in one direction (see Eq. 3). However, with this labeling method the original protein sample is diluted. Such a dilution may not be compatible ...
Oligomerization and activation of the FliI ATPase
... and our gel filtration and multiangle light scattering analysis of the native soluble enzyme confirmed this earlier work; all FliI was recovered as the monomeric form. However, when we incubated the enzyme with its substrate ATP, it was evident that FliI has a tendency to oligomerize. Electron micro ...
... and our gel filtration and multiangle light scattering analysis of the native soluble enzyme confirmed this earlier work; all FliI was recovered as the monomeric form. However, when we incubated the enzyme with its substrate ATP, it was evident that FliI has a tendency to oligomerize. Electron micro ...
Protein Synthesis
... large movement of the small ribosome subunit relative to the large subunit, the conformational changes that actually take place in the ribosome during translation are more subtle. It is likely that they involve a series of small rearrangements within each subunit as well as several small shifts betw ...
... large movement of the small ribosome subunit relative to the large subunit, the conformational changes that actually take place in the ribosome during translation are more subtle. It is likely that they involve a series of small rearrangements within each subunit as well as several small shifts betw ...
Expressway™ Cell-Free E. coli Expression System
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
Noll et al., 2007 - Institute of Molecular Life Sciences
... A potential model system for the study of embryonic cell adhesion and positional information (Wolpert and Gustafson, 1961; Noll et al., 1979) was introduced upon Herbst's discovery (1900), more than a century ago, that in calcium-free seawater sea urchin blastulae dissociate into single cells, which ...
... A potential model system for the study of embryonic cell adhesion and positional information (Wolpert and Gustafson, 1961; Noll et al., 1979) was introduced upon Herbst's discovery (1900), more than a century ago, that in calcium-free seawater sea urchin blastulae dissociate into single cells, which ...
Protein (nutrient)
Proteins are essential nutrients for the human body. They are one of the building blocks of body tissue, and can also serve as a fuel source. As a fuel, proteins contain 4 kcal per gram, just like carbohydrates and unlike lipids, which contain 9 kcal per gram. The most important aspect and defining characteristic of protein from a nutritional standpoint is its amino acid composition.Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken down in the stomach to smaller polypeptide chains via hydrochloric acid and protease actions. This is crucial for the synthesis of the essential amino acids that cannot be biosynthesized by the body.There are nine essential amino acids which humans must obtain from their diet in order to prevent protein-energy malnutrition. They are phenylalanine, valine, threonine, tryptophan, methionine, leucine, isoleucine, lysine, and histidine. There are five dispensable amino acids which humans are able to synthesize in the body. These five are alanine, aspartic acid, asparagine, glutamic acid and serine. There are six conditionally essential amino acids whose synthesis can be limited under special pathophysiological conditions, such as prematurity in the infant or individuals in severe catabolic distress. These six are arginine, cysteine, glycine, glutamine, proline and tyrosine.Humans need the essential amino acids in certain ratios. Some protein sources contain amino acids in a more or less 'complete' sense. This has given rise to various ranking systems for protein sources, as described in the article.Animal sources of protein include meats, dairy products, fish and eggs. Vegan sources of protein include whole grains, pulses, legumes, soy, and nuts. Vegetarians and vegans can get enough essential amino acids by eating a variety of plant proteins. It is commonly believed that athletes should consume a higher-than-normal protein intake to maintain optimal physical performance.