SISYPHUS—structural alignments for proteins with non
... structural alignments constructed for a set of proteins with known three-dimensional (3D) structures that have revealed non-trivial structural relationships and whose structural similarity is ambiguous when using standard methods for structure comparison. Protein domains are usually considered as di ...
... structural alignments constructed for a set of proteins with known three-dimensional (3D) structures that have revealed non-trivial structural relationships and whose structural similarity is ambiguous when using standard methods for structure comparison. Protein domains are usually considered as di ...
The Predicted Candidates of Arabidopsis Plastid Inner Envelope
... proteins are removed from our candidate list of 562, the remaining candidates are expected to be highly enriched in envelope proteins. After manual removal of 21 known thylakoid-associated proteins and their homologs, 541 proteins remained as potential candidates for Arabidopsis plastid envelope mem ...
... proteins are removed from our candidate list of 562, the remaining candidates are expected to be highly enriched in envelope proteins. After manual removal of 21 known thylakoid-associated proteins and their homologs, 541 proteins remained as potential candidates for Arabidopsis plastid envelope mem ...
learning objectives exam iii
... Understand the significance of catalytic efficiency Understand the derivation and utility of the Lineweaver-Burke plot in analyzing kinetic data from enzymatic reactions. Understand the different types of enzyme inhibition and how they affect kinetic constants. Be able to quickly generate Michaelis- ...
... Understand the significance of catalytic efficiency Understand the derivation and utility of the Lineweaver-Burke plot in analyzing kinetic data from enzymatic reactions. Understand the different types of enzyme inhibition and how they affect kinetic constants. Be able to quickly generate Michaelis- ...
- CSHL Institutional Repository
... Uta Raue‡§, Stefan Oellerer‡§, and Sabine Rospert‡1 From the ‡Institute of Biochemistry and Molecular Biology, Zentrum für Biochemie und Molekulare Zellforschung and the §Fakultät für Biologie, University of Freiburg, D-79104 Freiburg, Germany Ribosome-associated protein biogenesis factors (RPBs ...
... Uta Raue‡§, Stefan Oellerer‡§, and Sabine Rospert‡1 From the ‡Institute of Biochemistry and Molecular Biology, Zentrum für Biochemie und Molekulare Zellforschung and the §Fakultät für Biologie, University of Freiburg, D-79104 Freiburg, Germany Ribosome-associated protein biogenesis factors (RPBs ...
TPN-III-No acute stress
... 2. Provide 132 g of dextrose as a 6% concentration 2,200 mL. (132 g x 1000 ml/60 gm) 3. To stay within the guidelines for maximum dextrose and amino acid concentrations, the larger volume of 2,200 mL is used. Therefore, the final dextrose and amino acid concentrations are 6% and 2%, respectively. 4. ...
... 2. Provide 132 g of dextrose as a 6% concentration 2,200 mL. (132 g x 1000 ml/60 gm) 3. To stay within the guidelines for maximum dextrose and amino acid concentrations, the larger volume of 2,200 mL is used. Therefore, the final dextrose and amino acid concentrations are 6% and 2%, respectively. 4. ...
Word Count: 1390 An experiment to determine the amount of urea in
... I found the concentration of urea to be 0.58g/100 cm3. Figure 2.2 clearly shows that as the concentration of urea increases, the volume of HCl required for neutralisation also increases. This is to be expected as there are more moles of urea being hydrolysed, which would mean more HCl would be requi ...
... I found the concentration of urea to be 0.58g/100 cm3. Figure 2.2 clearly shows that as the concentration of urea increases, the volume of HCl required for neutralisation also increases. This is to be expected as there are more moles of urea being hydrolysed, which would mean more HCl would be requi ...
Enzyme Kinetics
... Pancreas, one of the most active organs in synthesizing & secreting proteins Acinar cell stimulated by hormonal signal or nerve impulse, granule content released into duct to duodenum ...
... Pancreas, one of the most active organs in synthesizing & secreting proteins Acinar cell stimulated by hormonal signal or nerve impulse, granule content released into duct to duodenum ...
NMR spectroscopy brings invisible protein states into
... turnover37,38,70, as the examples below illustrate. Finally, in one remarkable example it has been shown that a disordered molten globule of the enzyme chorismate mutase can, in the presence of substrate, fold into a structure capable of efficient catalysis71,72, which indicates at least in this cas ...
... turnover37,38,70, as the examples below illustrate. Finally, in one remarkable example it has been shown that a disordered molten globule of the enzyme chorismate mutase can, in the presence of substrate, fold into a structure capable of efficient catalysis71,72, which indicates at least in this cas ...
Evaluation of Different Solvent Types on the Extraction of
... al., 2014) and in (Chen et al., 2013) and further in (Taher et al., 2014), possess high disease resistance ability, high metabolites content and high biomass density (Roy and Pal, 2014). Compared to conventional farming, microalgal farming are characterized by a high productivity per unit area and t ...
... al., 2014) and in (Chen et al., 2013) and further in (Taher et al., 2014), possess high disease resistance ability, high metabolites content and high biomass density (Roy and Pal, 2014). Compared to conventional farming, microalgal farming are characterized by a high productivity per unit area and t ...
GluR-A C-terminal 10 residues constitute a binding motif
... Figure 1. a, Top, Representation of the chimeric bait comprising the proximal 40 amino residues of the GluR-B Cterminal domain (R-B) fused to the C-terminal 10 residues of GluR-A (R-At10); Gal4 BD, Gal4 DNA-binding domain. Bottom, Representation of RIL and the isolated RIL clones (numbers = amino re ...
... Figure 1. a, Top, Representation of the chimeric bait comprising the proximal 40 amino residues of the GluR-B Cterminal domain (R-B) fused to the C-terminal 10 residues of GluR-A (R-At10); Gal4 BD, Gal4 DNA-binding domain. Bottom, Representation of RIL and the isolated RIL clones (numbers = amino re ...
Amino acid contents and biological value of protein in various
... denatured (Correa et al., 1986; Imeri, 1987; Stratil, 1993). The conventional way of popping is done on hot plates at a temperature higher than 190°C (Tovar et al., 1989). Heat processing can cause damage of essential amino acids resulting in decreased contents or transfer into a racemic mixture (Br ...
... denatured (Correa et al., 1986; Imeri, 1987; Stratil, 1993). The conventional way of popping is done on hot plates at a temperature higher than 190°C (Tovar et al., 1989). Heat processing can cause damage of essential amino acids resulting in decreased contents or transfer into a racemic mixture (Br ...
Divalent Metal Ions in Plant Mitochondria and Their Role in
... that matrix metalloproteins such as Fe-S containing aconitase and Mn-containing superoxide dismutase (SOD) are sensitive to damage by H2O2. The observed metal losses are consistent with the fact that H2O2 is known to be able to damage proteins by fragmentation, destroying metal coordination sites (H ...
... that matrix metalloproteins such as Fe-S containing aconitase and Mn-containing superoxide dismutase (SOD) are sensitive to damage by H2O2. The observed metal losses are consistent with the fact that H2O2 is known to be able to damage proteins by fragmentation, destroying metal coordination sites (H ...
JOURNAL OF APPLIED SCIENCE AND AGRICULTURE
... studies that have proven its ability to extract high amount of high-quality protein (Hamada, 1998; Hamada, 2000; Hanmoungjai et al., 2001; Hanmoungjai et al., 2002; Tang et al., 2003), this process is not economically efficient due to the high cost of enzymes. Nevertheless, there are a few novel met ...
... studies that have proven its ability to extract high amount of high-quality protein (Hamada, 1998; Hamada, 2000; Hanmoungjai et al., 2001; Hanmoungjai et al., 2002; Tang et al., 2003), this process is not economically efficient due to the high cost of enzymes. Nevertheless, there are a few novel met ...
No Slide Title - Palm Beach State College
... – Define mixture and distinguish between mixtures and compounds. – Describe the biologically important properties of water. – Show how three kinds of mixtures differ from each other. – Discuss some ways in which the concentration of a solution can be expressed, and explain why different expressions ...
... – Define mixture and distinguish between mixtures and compounds. – Describe the biologically important properties of water. – Show how three kinds of mixtures differ from each other. – Discuss some ways in which the concentration of a solution can be expressed, and explain why different expressions ...
177 Chapter 26: Biomolecules: Amino Acids, Peptides, and Proteins
... R. Bruce Merrifield, Rockefeller University, 1984 Nobel Prize in Chemistry: “for his development of methodology for chemical synthesis on a solid matrix.” ...
... R. Bruce Merrifield, Rockefeller University, 1984 Nobel Prize in Chemistry: “for his development of methodology for chemical synthesis on a solid matrix.” ...
Finals Practice Exam
... 37). ATP and other energetic phosphorylated compounds are said to possess a phosphoryl transfer potential. Other molecules in the cell are capable of storing potential energy for transferring other chemical groups. What molecule was encountered in our study of metabolism that possesses a different t ...
... 37). ATP and other energetic phosphorylated compounds are said to possess a phosphoryl transfer potential. Other molecules in the cell are capable of storing potential energy for transferring other chemical groups. What molecule was encountered in our study of metabolism that possesses a different t ...
Sources of enzyme
... not highly purified. They contain a number of enzymes with different catalytic functions and are not used with either a pure substrate or a completely defined ...
... not highly purified. They contain a number of enzymes with different catalytic functions and are not used with either a pure substrate or a completely defined ...
The Logic Linking Protein Acetylation and Metabolism
... deacetylases. This model suggests that protein acetylation regulates metabolic strategy and also helps store energy in cells. It is now apparent that a large number of cellular proteins are acetylated. For example, recent studies have uncovered acetylation of a high fraction of mitochondrial protein ...
... deacetylases. This model suggests that protein acetylation regulates metabolic strategy and also helps store energy in cells. It is now apparent that a large number of cellular proteins are acetylated. For example, recent studies have uncovered acetylation of a high fraction of mitochondrial protein ...
immuno 2015 - hippocampus
... species. However, if there are proteins in the tissue that you’re labeling that are similar to that of primary host species, the secondary might bind to those proteins, and give you a false positive. The blocking step eliminates potential endogenous binding sites for the secondary antibodies, leavin ...
... species. However, if there are proteins in the tissue that you’re labeling that are similar to that of primary host species, the secondary might bind to those proteins, and give you a false positive. The blocking step eliminates potential endogenous binding sites for the secondary antibodies, leavin ...
Chapter 23: Meat Emulsions - FEA
... homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpol ...
... homogenization; it forms a membrane that protects the globules against aggregation when they come close to each other (McClements 2005). In a number of cases, emulsifiers are necessary to facilitate emulsion formation (Schramm 2005). Many emulsifiers are amphiphilic molecules, with polar and nonpol ...
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins
... the following assertion: NGF is most likely identical to a hydrophobin dimer. According to their strong surface activity hydrophobins are able to induce gushing in beer. Furthermore, the rodlet layers with their typical rough surfaces may serve as condensation nuclei for the release of CO2-bubbles. ...
... the following assertion: NGF is most likely identical to a hydrophobin dimer. According to their strong surface activity hydrophobins are able to induce gushing in beer. Furthermore, the rodlet layers with their typical rough surfaces may serve as condensation nuclei for the release of CO2-bubbles. ...
structure and function of bio- molecules - Aditya K Panda, PhD
... Structure and function of Biomolecules Fig. 2.5 Four levels of organization of a protein. Proteins tend to have a complex three-dimensional structure. If one considers the number of atoms and bonds in a typical protein it is amazing to note that normally each protein has a single (sometimes a few) ...
... Structure and function of Biomolecules Fig. 2.5 Four levels of organization of a protein. Proteins tend to have a complex three-dimensional structure. If one considers the number of atoms and bonds in a typical protein it is amazing to note that normally each protein has a single (sometimes a few) ...
TIBS review article by Killian & Heijne
... and compared with partitioning in octanol or cyclohexane. In this way, the energetics of the interface interaction of several side chains was characterized in detail. It was found that in particular Trp and Tyr have a special affinity for the interface. To obtain insight into the physical basis unde ...
... and compared with partitioning in octanol or cyclohexane. In this way, the energetics of the interface interaction of several side chains was characterized in detail. It was found that in particular Trp and Tyr have a special affinity for the interface. To obtain insight into the physical basis unde ...
Protein
Proteins (/ˈproʊˌtiːnz/ or /ˈproʊti.ɨnz/) are large biomolecules, or macromolecules, consisting of one or more long chains of amino acid residues. Proteins perform a vast array of functions within living organisms, including catalyzing metabolic reactions, DNA replication, responding to stimuli, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific three-dimensional structure that determines its activity.A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than about 20-30 residues, are rarely considered to be proteins and are commonly called peptides, or sometimes oligopeptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residues in a protein is defined by the sequence of a gene, which is encoded in the genetic code. In general, the genetic code specifies 20 standard amino acids; however, in certain organisms the genetic code can include selenocysteine and—in certain archaea—pyrrolysine. Shortly after or even during synthesis, the residues in a protein are often chemically modified by posttranslational modification, which alters the physical and chemical properties, folding, stability, activity, and ultimately, the function of the proteins. Sometimes proteins have non-peptide groups attached, which can be called prosthetic groups or cofactors. Proteins can also work together to achieve a particular function, and they often associate to form stable protein complexes.Once formed, proteins only exist for a certain period of time and are then degraded and recycled by the cell's machinery through the process of protein turnover. A protein's lifespan is measured in terms of its half-life and covers a wide range. They can exist for minutes or years with an average lifespan of 1–2 days in mammalian cells. Abnormal and or misfolded proteins are degraded more rapidly either due to being targeted for destruction or due to being unstable.Like other biological macromolecules such as polysaccharides and nucleic acids, proteins are essential parts of organisms and participate in virtually every process within cells. Many proteins are enzymes that catalyze biochemical reactions and are vital to metabolism. Proteins also have structural or mechanical functions, such as actin and myosin in muscle and the proteins in the cytoskeleton, which form a system of scaffolding that maintains cell shape. Other proteins are important in cell signaling, immune responses, cell adhesion, and the cell cycle. Proteins are also necessary in animals' diets, since animals cannot synthesize all the amino acids they need and must obtain essential amino acids from food. Through the process of digestion, animals break down ingested protein into free amino acids that are then used in metabolism.Proteins may be purified from other cellular components using a variety of techniques such as ultracentrifugation, precipitation, electrophoresis, and chromatography; the advent of genetic engineering has made possible a number of methods to facilitate purification. Methods commonly used to study protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic resonance and mass spectrometry.