Protein Synthesis Inhibitors
... different members of the class created by modification at selected carbon sites. Mechanism of Action: The primary target is the 30S ribosome, preventing binding of aminoacyl tRNA to the acceptor (A) site on mRNA. Mechanism of Resistance: The primary mechanism of resistance to tetracyclines is decrea ...
... different members of the class created by modification at selected carbon sites. Mechanism of Action: The primary target is the 30S ribosome, preventing binding of aminoacyl tRNA to the acceptor (A) site on mRNA. Mechanism of Resistance: The primary mechanism of resistance to tetracyclines is decrea ...
RNA Synthesis and Splicing
... - binds tightly (intercalates) to ds-DNA (cannot be template for RNA synthesis) - its ability to inhibit growth of rapid dividing cells makes it a effective agent in cancer treatment ...
... - binds tightly (intercalates) to ds-DNA (cannot be template for RNA synthesis) - its ability to inhibit growth of rapid dividing cells makes it a effective agent in cancer treatment ...
Organic compounds
... Draw Figure 3.5 phospholipid. Label the head and the tail. 1. How would the polar head of a phospholipid respond to water molecules? 2. How would the nonpolar tails respond to water molecules? ...
... Draw Figure 3.5 phospholipid. Label the head and the tail. 1. How would the polar head of a phospholipid respond to water molecules? 2. How would the nonpolar tails respond to water molecules? ...
Summary of Organic chemistry
... Good solvent for other organic cpnds Ethoxyethane (diethyl ether) used as anaesthetic -ethanoic (acetic) acid produced by fermentation of fruit sugar ethanol ethanoic acid (enzyme req'd) -synthesized from ethyne (acetylene) - most esters have pleasant, fruity flavours -natural and synthetic flav ...
... Good solvent for other organic cpnds Ethoxyethane (diethyl ether) used as anaesthetic -ethanoic (acetic) acid produced by fermentation of fruit sugar ethanol ethanoic acid (enzyme req'd) -synthesized from ethyne (acetylene) - most esters have pleasant, fruity flavours -natural and synthetic flav ...
Amino Acids - The Exam Formula
... They use the Rf value to try to identify which amino acids are present as each should have a slightly different Rf value. The problem with this is of course that many things could ...
... They use the Rf value to try to identify which amino acids are present as each should have a slightly different Rf value. The problem with this is of course that many things could ...
Word document format
... In the above reaction, you must nitrate the ring first, since a methyl group is an ortho/para director. After nitration, oxidize the methyl group to a carboxylic acid. Notice that oxidation, followed by nitration versus nitration followed by oxidation gave very different products. 6. You must practi ...
... In the above reaction, you must nitrate the ring first, since a methyl group is an ortho/para director. After nitration, oxidize the methyl group to a carboxylic acid. Notice that oxidation, followed by nitration versus nitration followed by oxidation gave very different products. 6. You must practi ...
Targeted Quantitation of HMGB1 Protein by label
... liquid chromatography (LC-MS): these methods, which includes a number of well-established options like MS-based methods, coupled to protein (or peptide) separation and bioinformatics analysis allow the achievement of protein identification and quantification [1]. The identification of protein PTMs i ...
... liquid chromatography (LC-MS): these methods, which includes a number of well-established options like MS-based methods, coupled to protein (or peptide) separation and bioinformatics analysis allow the achievement of protein identification and quantification [1]. The identification of protein PTMs i ...
In the nucleus
... Intron- internal segment of mRNA that does not code for protein. Exon- Segments of mRNA that code for proteins remain after splicing. Splicing- removal of introns and rejoining of cut exons. Splicing enzymes recognize GU at one end of an intron and AG at the other end. ...
... Intron- internal segment of mRNA that does not code for protein. Exon- Segments of mRNA that code for proteins remain after splicing. Splicing- removal of introns and rejoining of cut exons. Splicing enzymes recognize GU at one end of an intron and AG at the other end. ...
Molecules Most important molecules are based on carbon. Carbon
... Some amino acids are hydrophyllic (like water), others hydrophobic (dislike water) Amino acids differ in what is termed their “R” group [OVERHEAD, fig. 3.11B, p. 42]. All the stuff between the caroboxyl and amino groups (except the carbon that ties everything together). As mentioned, only 20 amino a ...
... Some amino acids are hydrophyllic (like water), others hydrophobic (dislike water) Amino acids differ in what is termed their “R” group [OVERHEAD, fig. 3.11B, p. 42]. All the stuff between the caroboxyl and amino groups (except the carbon that ties everything together). As mentioned, only 20 amino a ...
PG1005 Lecture 17 Gene Transcription
... -AGCU (adenine, guanine, cytosine and uracil) -Intramolecular complimentary sequences found in RNA Can form intramolecular bonds permitting folding and generation of precise 3D structures ...
... -AGCU (adenine, guanine, cytosine and uracil) -Intramolecular complimentary sequences found in RNA Can form intramolecular bonds permitting folding and generation of precise 3D structures ...
Three-Point Binding Model
... template synthesis): Ribosome holds pieces together Ribosome is cellular “workbench” ...
... template synthesis): Ribosome holds pieces together Ribosome is cellular “workbench” ...
Document
... • N-formylmethionine in prokaryotes is _______________ • specific bonds in precursors are cleaved, as for example, preproinsulin to proinsulin to insulin • ___________ _________are removed by specific proteases of the endoplasmic reticulum; the Golgi apparatus then directs the finished protein to it ...
... • N-formylmethionine in prokaryotes is _______________ • specific bonds in precursors are cleaved, as for example, preproinsulin to proinsulin to insulin • ___________ _________are removed by specific proteases of the endoplasmic reticulum; the Golgi apparatus then directs the finished protein to it ...
Biosynthesis of Pyrimidines
... More Pyrimidine Synthesis • Step 3: ring closure and dehydration catalyzed by dihydroorotase • Step 4: Synthesis of a true pyrimidine (orotate) by DHO dehydrogenase • Step 5: Orotate is joined with a ribose-P to form orotidine-5’-phosphate • The ribose-P donor is PRPP • Step 6: OMP decarboxylase ma ...
... More Pyrimidine Synthesis • Step 3: ring closure and dehydration catalyzed by dihydroorotase • Step 4: Synthesis of a true pyrimidine (orotate) by DHO dehydrogenase • Step 5: Orotate is joined with a ribose-P to form orotidine-5’-phosphate • The ribose-P donor is PRPP • Step 6: OMP decarboxylase ma ...
L3 - DNA Translation (Protein Synthesis
... tRNA molecule carrying the N-terminal amino acid (usually a form of methionine) meets with the mRNA molecule on a ribosome forming a complex at a specific site called the peptidyl site, or P site. ...
... tRNA molecule carrying the N-terminal amino acid (usually a form of methionine) meets with the mRNA molecule on a ribosome forming a complex at a specific site called the peptidyl site, or P site. ...
RNA Transcription
... once 'information' has passed into protein it cannot get out again. The transfer of information from nucleic acid to nucleic acid, or from nucleic acid to protein, may be possible, but transfer from protein to protein, or from protein to nucleic acid, is ...
... once 'information' has passed into protein it cannot get out again. The transfer of information from nucleic acid to nucleic acid, or from nucleic acid to protein, may be possible, but transfer from protein to protein, or from protein to nucleic acid, is ...
Introduction and Review
... Four different groups are attached to the central carbon atom (α-carbon) Hydrogen atom Amino group (-N+H3) Carboxylic acid group (-COO-) Side chain group (“-R”): 20 different amino acids, each with a different side ...
... Four different groups are attached to the central carbon atom (α-carbon) Hydrogen atom Amino group (-N+H3) Carboxylic acid group (-COO-) Side chain group (“-R”): 20 different amino acids, each with a different side ...
prot_synth_inhib_Low..
... different members of the class created by modification at selected carbon sites. Mechanism of Action: The primary target is the 30S ribosome, preventing binding of aminoacyl tRNA to the acceptor (A) site on mRNA. Mechanism of Resistance: The primary mechanism of resistance to tetracyclines is decrea ...
... different members of the class created by modification at selected carbon sites. Mechanism of Action: The primary target is the 30S ribosome, preventing binding of aminoacyl tRNA to the acceptor (A) site on mRNA. Mechanism of Resistance: The primary mechanism of resistance to tetracyclines is decrea ...
Non-natural amino acid
... Chemical synthesis (with or without chemical ligation) can be used introduce a range of functional groups chemical synthesis is often not economical and limited to short peptides need to fold the protein following synthesis and purification ...
... Chemical synthesis (with or without chemical ligation) can be used introduce a range of functional groups chemical synthesis is often not economical and limited to short peptides need to fold the protein following synthesis and purification ...
Molecular_files/Translation Transcription
... – A codon = a 3 nucleotide base sequence – Each codon codes for an amino acid – Should have 64 different codons (4 nucleotide choices, 3 bases) but only 20 amino acids- why? ...
... – A codon = a 3 nucleotide base sequence – Each codon codes for an amino acid – Should have 64 different codons (4 nucleotide choices, 3 bases) but only 20 amino acids- why? ...
Bottromycin
Bottromycin is a macrocyclic peptide with antibiotic activity. It was first discovered in 1957 as a natural product isolated from Streptomyces bottropensis. It has been shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) among other Gram-positive bacteria and mycoplasma. Bottromycin is structurally distinct from both vancomycin, a glycopeptide antibiotic, and methicillin, a beta-lactam antibiotic.Bottromycin binds to the A site of the ribosome and blocks the binding of aminoacyl-tRNA, therefore inhibiting bacterial protein synthesis. Although bottromycin exhibits antibacterial activity in vitro, it has not yet been developed as a clinical antibiotic, potentially due to its poor stability in blood plasma. To increase its stability in vivo, some bottromycin derivatives have been explored.The structure of bottromycin contains a macrocyclic amidine as well as a thiazole ring. The absolute stereochemistry at several chiral centers has been determined as of 2009. In 2012, a three-dimensional solution structure of bottromycin was published. The solution structure revealed that several methyl groups are on the same face of the structure.Bottromycin falls within the ribosomally synthesized and post-translationally modified peptide class of natural product.