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Full text
Full text

... of diseases in space and time. They are carried out relying on mathematical models and their analysis’ tools; mathematic aids in inferring disease causes, predicting the future course of an outbreak and planning the most appropriate control measures. The most numerous epidemiological mathematical mo ...
2q13 microdeletions
2q13 microdeletions

... children may benefit from their use. First of all, it is strongly believed that a good routine, where your child goes to sleep at the same time in the same place every night (when possible) does help. Having a habitual ‘going to bed’ routine that is neither too long nor too short may also help. For ...
Testing Methylation Pathways
Testing Methylation Pathways

... added to specific elements of DNA, our gene markers and proteins that keep them physiologically active. Methylation is a major pathway to focus on in understanding autoimmune and neurological diseases such as multiple ...
What is Cytogenetics?
What is Cytogenetics?

... • In this example, red arrows point to identical bands on each chromosome. The blue arrow points to a duplication of the band at the red arrow. The chromosome on the right is longer. • 46,XY,dup(7)(q11.2q22) • Male with a duplication of chromosome 7 on the long arm (q) between bands 11.2 to 22. ...
Novel regulatory roles of cAMP receptor proteins in
Novel regulatory roles of cAMP receptor proteins in

... each set of biological replicates before expression ratios were calculated. The results from four biological replicates, which included two dye swaps, were then subjected to a t-test without false discovery correction in The Institute of Genomic Research MeV software (version 4.3.02). The mean value ...
Small aminoacyl transfer centers at GU within a larger RNA
Small aminoacyl transfer centers at GU within a larger RNA

... Thus, multiple GU…. aminoacyl transfer sites appear to be observable in a larger RNA, though there are reactive, probably favorably structured sites as well as those that appear unreactive, probably less favorably structured, ranging down to sites with experimentally insignificant activity. Selecti ...
Türkiye, İzmir Sokak Kedilerinde Neonatal İzoeritrolizisle İlişkili
Türkiye, İzmir Sokak Kedilerinde Neonatal İzoeritrolizisle İlişkili

... highest prevalence (85-100%) worldwide. In some regions, the prevalence of type B cats increases such as Greece, England, Australia, and Turkey [17]. ...
SAB-2010
SAB-2010

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bioinformatics
bioinformatics

... 2001). Missing log2 transformed data are often replaced by zeros (Alizadeh et al., 2000) or, less often, by an average expression over the row, or ‘row average’. This approach is not optimal, since these methods do not take into consideration the correlation structure of the data. Thus, many analysi ...
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... • The k-tuple length is user-defined and is usually 1 or 2 for protein sequences (i.e. either the positions of each of the individual 20 amino acids or the positions of each of the 400 possible dipeptides are located). • For nucleic acid sequences, the k-tuple is 5-20, and should be longer because s ...
Classifying Gene Expression Data using an Evolutionary Algorithm
Classifying Gene Expression Data using an Evolutionary Algorithm

... in labelling or detecting fluorescent dyes, and systematic biases in the expression level measurements (Quckenbush, 2002). Many of these factors make distinctions between differentially and constantly expressed genes difficult. The normalization process must be done on with the log-ratio values befo ...
Hardy-Weinberg Extension for the Whirling Allele
Hardy-Weinberg Extension for the Whirling Allele

... In a system in equilibrium, a “steady state” exists until external forces or factors are introduced to disturb the equilibrium. Thus, in a system in equilibrium there is no change in gene pool. To maintain equilibrium in a system no new genes must enter the gene pool, mating must be random, mutation ...
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... biotype 3. In addition, two novel genotypes 1 and 2 are proposed for the current biotypes 1 and 2 of P. zopfii. For this study, P. zopfii isolates from bovine mastitis from different herds (RZII-2 and RZII-3), from different cattle liquid manure and cattle barns (RZI-1, RZI-2 and RZI-3), from differ ...
Brooker Chapter 8
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x/xy chromosome mosaicism: turner syndrome and

... gonadal dysgenesis (McElreavey, 1996). Many publications report affected relatives, most of them being two sisters. Sex-reversal mutation analysis in a unique family with three affected sisters was recently reported by us (Just et al., 2007). We excluded mutation in SRY and flanking sequences, also ...
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Missing value estimation methods for DNA microarrays.
Missing value estimation methods for DNA microarrays.

... 2001). Missing log2 transformed data are often replaced by zeros (Alizadeh et al., 2000) or, less often, by an average expression over the row, or ‘row average’. This approach is not optimal, since these methods do not take into consideration the correlation structure of the data. Thus, many analysi ...
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the purine-pyrimidine classification scheme reveals new

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LDheatmap (Version 0.9-1): Example of Adding Tracks

... give information on the SNPs, such as their alleles and genomic location. The help file help("GIMAP5.CEU") gives full details. In addition to GIMAP5.CEU, you should have the LDheatmap objects llGenes and llGenesRecomb in your workspace. These objects are the heatmap with tracks for genes and recombi ...
Amelioration of Sardinian 0 thalassemia by genetic
Amelioration of Sardinian 0 thalassemia by genetic

... were generally insignificant, with only one barely significant interaction observed. Larger studies are necessary to confirm this epistatic effect. The SNP variant in the BCL11A gene contributed more strongly than the HBS1L-MYB locus or the coinheritance of ␣-thalassemia. This is in agreement with p ...
TNT® T7 Quick for PCR DNA Technical Manual
TNT® T7 Quick for PCR DNA Technical Manual

... The ability to directly analyze PCR products with the TnT® T7 Quick for PCR DNA system is highly advantageous. The quality of the results is dependent on the ability to obtain discrete, specific PCR products. The selection of primers is an important first step in this process. Many researchers now u ...
Comparative genomic analysis of carbon and nitrogen assimilation
Comparative genomic analysis of carbon and nitrogen assimilation

... bioleaching bacteria isolated from mine sites in Chile was performed. This study demonstrated that there are important differences in the carbon dioxide and nitrogen fixation mechanisms among bioleaching bacteria that coexist in mining environments. Results: In this study, we probed that both Acidit ...
Visualisation of Reduced-Dimension Microarray Data Using
Visualisation of Reduced-Dimension Microarray Data Using

This article was published in an Elsevier journal. The attached copy
This article was published in an Elsevier journal. The attached copy

... 10 min, the bioluminescent signals were quantified and recorded using an IVIS Imaging System (Xenogen) following the manufacturer’s instructions. The conditioned cell culture media (harvested before the luciferin addition) was returned to the same plate for further incubation. Measurements from the ...
פרויקט מחקר - בנימין קפא
פרויקט מחקר - בנימין קפא

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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