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Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.
Maimbo, M., Ohnishi, K., Hikichi, Y., Yoshioka, H. and Kiba, A.

... RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its ...
DNA Crystallography
DNA Crystallography

and GvpD-mediated transcription regulation of the p
and GvpD-mediated transcription regulation of the p

... and growth conditions (Pfeifer et al., 2001), the p-vac construct containing the entire p-vac region (Offner & Pfeifer, 1995), and the Eex and pA-bgaH constructs (Gregor & Pfeifer, 2001) have been described previously. The A construct contained a 500 bp XbaI– BamHI p-gvpA fragment (position 21–520 i ...
TAS2R38 - GenoVive
TAS2R38 - GenoVive

PDF
PDF

... Achim Haecker1, Mattias Bergman1, Christine Neupert2, Bernard Moussian3, Stefan Luschnig3,*, Markus Aebi2 and Mattias Mannervik1,† N-linked glycosylation is a prevalent protein modification in eukaryotic cells. Although glycosylation plays an important role in cell signaling during development, a ro ...
Biology 3A Laboratory Mendelian, Human and Population Genetics
Biology 3A Laboratory Mendelian, Human and Population Genetics

... After meiosis (sexual reproduction), the genetic traits of an organism are segregated and readied to be passed from parent to offspring. When sexually reproducing organisms undergo meiosis, they produce gametes that are haploid (n). Haploid cells include sperm and ovum (unfertilized egg) that have h ...
Table 1
Table 1

... Whole genome amplification is a useful tool, and possibly a necessary step, for amplifying unique and rare samples in order to have enough input DNA material for use with current technology. This study has evaluated the practical use of several commercially available WGA kits in relation to ease of ...
Glimpses of a few literatures on snRNA
Glimpses of a few literatures on snRNA

... Number of sequenced examples is a snapshot as of 2002 and is influenced by DNA-sequencing strategies and database upkeep; it may provide a rough indication of relative abundance. RNAs in any group vary in size; the size provided here indicates the lower end of the length distribution for the natura ...
Leukaemia Section dup(21q) amplified (RUNX1) Atlas of Genetics and Cytogenetics
Leukaemia Section dup(21q) amplified (RUNX1) Atlas of Genetics and Cytogenetics

Agarose Gel Electrophoresis
Agarose Gel Electrophoresis

... large DNA fragments, as shown in Figure 2.5A.2B. For separating large DNA molecules, it is best to run gels at both low agarose concentrations and low applied voltages (∼1 V/cm, 0.5% agarose). Electrophoresis buffers. The two most widely used electrophoresis buffers are Tris/ acetate (TAE) and Tris/ ...
A study of anticipation in families with hereditary non
A study of anticipation in families with hereditary non

... Colorectal cancer (CRC), cancer in the colon and rectum, is found and diagnosed all over the world and is actually the third most common form of cancer (Parkin et al. 2005). Men and women have a similar risk of developing a tumor, 1.2:1, respectively (Parkin et al. 2005). In Sweden on the average 56 ...
Bio 111 Handout for Genetics 1 Bio 111 iClicker Question #1
Bio 111 Handout for Genetics 1 Bio 111 iClicker Question #1

... In lecture, I talked about “counting unrelated carriers” - finding out how many people had to bring in a disease allele to explain a particular pedigree. This was useful in the case where more than one mode of inheritance was possible but you were asked to determine which was more likely. There are ...
Nature, nurture and mental disorder
Nature, nurture and mental disorder

... of the polymerase chain reaction and automated methods of analysis, have resulted in the mapping of a dense web of polymorphic genetic markers. As the number of identified susceptibility genes, and the databases on their associations with specific diseases, grow more rapidly, new problems are being ...
The role of mutations in core protein of hepatitis B
The role of mutations in core protein of hepatitis B

... related liver diseases. Patients with long standing active liver disease are at high risk to develop liver cirrhosis or hepatocellular carcinoma [3]. The genome of hepatitis B virus encodes four overlapping open reading frames that are translated to viral core protein or HBc particle, the surface pr ...
Structural Energetics of a RNA-DNA Hybrid
Structural Energetics of a RNA-DNA Hybrid

... where G complex is the net free energy that stabilizes the elongation complex against dissociation, G DNA DNA is the unfavorable free energy of opening the DNA-DNA base pairs, G  DNA RNA is the favorable free energy of forming the DNA-RNA base pairs in the hybrid, and G NA poylmerase is t ...
004 - cse.sc.edu
004 - cse.sc.edu

... • Automate computer work that you do by hand save time & reduce errors • Run the same analysis on lots of similar data files = scale-up • Analyze data, make decisions • sort Blast results by e-value &/or species of best mach ...
Activated Sugar Precursors: Biosynthetic Pathways and Biological
Activated Sugar Precursors: Biosynthetic Pathways and Biological

Final Thesis Solid-phase bio-organic synthesis to create intelligent surfaces Patrik Nygren
Final Thesis Solid-phase bio-organic synthesis to create intelligent surfaces Patrik Nygren

... The ability to tailor make surfaces with well-defined chemical properties has been, and still is, a major research field. Well-defined surfaces can be used to investigate biological systems (protein chips), to create nano-devices (nano-electronics), to design new materials etc. the applications are ...
Evaluation_ofDot - African Index Medicus
Evaluation_ofDot - African Index Medicus

... significant delays.(5) In light of the worsening global TB epidemic and the extreme vulnerability of HIV-infected individuals to TB, rapid and reliable antimicrobial susceptibility testing in the laboratory is paramount for proper management of patients, particularly those with multi-drug resistant ...
- Iranian Journal of Basic Medical Sciences
- Iranian Journal of Basic Medical Sciences

... 20. Mohajeri P, Tavakoli A, Moghim S. Detection of mutation at codon 315 KatG gene as a gene marker associated with isoniazid resistance, in Mycobacterium tuberculosis strains isolated from patients in Isfahan and Tehran by PCR-RFLP method. J Zanjan Unive Med Sci Health Services 2009; 17:29-40. 21. ...
Lesson 17: Patterns of Inheritance (3
Lesson 17: Patterns of Inheritance (3

... phenotype is the physical expression of the traits. The phenotype does not necessarily reveal the combination of alleles and can be influenced by the environment surrounding an organism. For example, the genotype determining a person’s height is a range, such as 5’4” to 5’6”. The nutrition a person ...
Drought response in the spikes of barley: gene expression in the
Drought response in the spikes of barley: gene expression in the

The bald and the beautiful: hairlessness in domestic dog breeds
The bald and the beautiful: hairlessness in domestic dog breeds

... on the dome of the head, extremities and tail. These dogs have additional health issues, including defects in teeth, nails, sweat glands, etc. [31,32]. The overall phenotype is similar to what is observed in the Xolo and Peruvian Inca Orchid; however, these breeds often lack the long hair tufts of t ...
Y chromosome polymorphisms in medicine
Y chromosome polymorphisms in medicine

... ®rst description in 1921 (1), the scienti®c community has been faced with divergent opinions about its role and importance in humans. Due to the abundance of tandemly repeated satellite DNA and the apparent paucity of gene content, the Y chromosome was considered for a long time to be a `genetic was ...
Molecular Plant-Microbio Interactions
Molecular Plant-Microbio Interactions

... minosarum strain mutated in a chromosomal locus that acts negatively on nodulation of Pisum sativum and Vicia hirsuta. Strain RBL5523 is a derivative of R. leguminosarum bv. trifolii RCR5 in which the Sym plasmid is replaced by the R. leguminosarum bv. viciae Sym plasmid pRL1JI (Table 1) and, theref ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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