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Recombinant DNA Technologies
Recombinant DNA Technologies

... C. Gene Therapy 1. Problems with gene expression lead to faulty molecules, especially enzymes -cannot do their jobs 2. examples: Cystic Fibrosis & Tay Sachs diseases 3. Idea: replace bad genes with good ones that make the proper molecule  And theoretically “fix” the problem 4. How can we deliver t ...
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... • Are tightly wound coils of DNA. Chromosomes can be seen in a light microscope but in order to see the DNA you have to have a high powered mircroscope ...
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Chapter 11

... explaining how DNA leads to protein synthesis.  Review the history of this scientific quest for knowledge. ...
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... PCR can be used to specifically target gene of interest – “Enzymatic amplification of specific DNA fragment using repeated cycles of DNA denaturation, primer annealing and ...
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14-3 Human Molecular Genetics

... is replaced by a normal, working gene. - This way the body can make the correct protein or enzyme it needs, which eliminates the cause of the disorder. ...
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Expanded Genetic Code in a Bacterium

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... Ligase: joins (seals) the ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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