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Genetics and Genetic Engineering
Genetics and Genetic Engineering

... change the function of cells by inserting their DNA into the DNA of the cell ...
E coli
E coli

... • Bacterial chromosome is a large (4 Mb in E coli) circular molecule • Bacterial cells may also contain small circular chromosomes called plasmids (4kb - 100kb; 1 - 1000 copies) that code for optional functions such as antibiotic resistance • Will look at circular DNA in this lecture • The bacterial ...
Learning Guide:
Learning Guide:

... o Describe how gene regulation is like conducting an orchestra.  Bacteria often respond to environmental change by regulating transcription o Explain why bacteria express only the genes whose products are needed by the cell. o List the three parts of an operon and explain the role of each one. o Di ...
Gene Expression
Gene Expression

... and effectively remove it before it can be translated into protein • Exon shuffling – one gene encodes for different proteins – depends on which exons translation machinery “keeps” • Protein folding – one protein can be folded differently to have different functions – depends on enzymes and chaperon ...
DNA/Strawberry Lab Write the question and answers on your own
DNA/Strawberry Lab Write the question and answers on your own

... will filter out of your solution and you will actually see DNA. Write a brief description of what you think the DNA will look like. _____________________________________________________________________________ ANSWER THESE QUESTIONS AFTER YOU FINISH THE LAB: 1. What was the purpose of mashing the st ...
Reverse genetics - From protein or RNA to gene Up until
Reverse genetics - From protein or RNA to gene Up until

... regulating different cellular functions, but share sequences based on the activity of one component as a histidine protein kinase, and of the other component as a substrate. Degenerate PCR primers designed to match the consensus sequences of conserved motifs have been used to isolate genes encoding ...
Lecture 3: More Transmission Genetics
Lecture 3: More Transmission Genetics

... (indicates a dominant disease) and males and females are both about equally affected (indicates autosomal inheritance) ...
Problem Set
Problem Set

... baldness in humans. After reading several reviews on DNA microarray technology, you decide that performing microarray studies on skin biopsies from wookie starwarius would be your method of choice for this work. However, these studies pose certain technical and theoretical problems: 1) Being a new s ...
9 Genomics and Beyond
9 Genomics and Beyond

... (2) The DNA fragments must overlap other fragments, so the restriction enzyme is not allowed to cut at every possible restriction site. (3) Computers assemble the fragments into contigs by determining which DNA pieces have bands that are common. (4) Unique regions of DNA can also be used to identify ...
DNA Control Mechanisms
DNA Control Mechanisms

... D. Heterochromatin - This refers to DNA that remains condensed even during interphase. – It is NOT active. 1. This CANNOT do transcription so it is inactivated. (“hetero” means “different”) E. Euchromatin - This refers to DNA that IS loose during interphase. – It IS active. 1. It CAN do transcriptio ...
Microarray technique and Functional genomics
Microarray technique and Functional genomics

... Wenjing Tao University of Missouri ...
Ab_initio_predition_tools - Compgenomics2010
Ab_initio_predition_tools - Compgenomics2010

... • Viterbi algorithm then calculates the functional sequence X* such that P(X*|S) is the largest among all possible values of X. • Ribosome binding site model was also added to augment accuracy in the prediction of translational start sites. ...
DNA Sequencing
DNA Sequencing

... Chemical degradation of purified fragments (chemical degradation) The single stranded DNA fragment to be sequenced is end-labeled by treatment with alkaline phosphatase to remove the 5’phosphate It is then followed by reaction with P-labeled ATP in the presence of polynucleotide kinase, which att ...
ANSWER KEY Nucleic Acid and DNA Replication Outline Notes
ANSWER KEY Nucleic Acid and DNA Replication Outline Notes

... CYTOSINE bonds with GUANINE ...
Microbial Genetics
Microbial Genetics

... • Gene product refers to the actual structures, enzymes or regulators. • Most gene products will be a polypeptide, which fold-up into functions proteins. • The instructions are manifest as a unique sequence of nucleotide base pairs within a larger DNA molecule. • A universal genetic code is followed ...
Nucleic Acids and DNA Replication
Nucleic Acids and DNA Replication

... • A always to T (with 2 hydrogen bonds) • C always to G (with 3 hydrogen bonds) • Chargraff’s Rule: for any given species the % of Ts will by equivalent of the % of As while the % of Cs will be equivalent to the % of Gs ...
Chromatin Structure and Function
Chromatin Structure and Function

... B. Histone H1 is involved in 30 nm fiber formation. Binds to DNA and surface of nucleosome and helps condense chromatin. ...
Genome Annotation - Virginia Commonwealth University
Genome Annotation - Virginia Commonwealth University

... Match genomic DNA to genes that have been previously cloned and sequenced looking for sequence similarity using BLAST programs Predict genes using computer programs to scan genomic DNA using known elements Many strategies use a combination of both methods ...
B3a Worksheet 3: DNA - School
B3a Worksheet 3: DNA - School

... Genetic engineering – cut and paste! Here is a description of how bacteria can be genetically engineered to produce human insulin. Insulin is needed for the treatment of diabetics. Many bacterial cells contain rings of DNA, which are called plasmids. These plasmids can be used in genetic engineering ...
Document
Document

... A certain Drosophila protein-coding gene has one intron. If a large sample of null alleles of the gene is examined, will any of the mutant sites be EXPECTED: First, what is a null mutation?! A null mutation is one that results in complete absence of function of the geneResults in no protein functio ...
Human Genome Project
Human Genome Project

... generate a probe. – Extract mRNA – Reverse transcribe it (RNA  complementary DNA ...
How do the specific expressions of genes compare between
How do the specific expressions of genes compare between

Slide 1
Slide 1

... Cell wall contains openings that let food go in and waste goes out through its pores. ...
Bio 262- Genetics Study Guide
Bio 262- Genetics Study Guide

... (T). In nature, base pairs form only between A and T and between G and C; thus the base sequence of each single strand can be deduced from that of its partner. DNA fingerprinting: a unique pattern of dna fragments as revealed by southern hybridization or by the polymerase chain reaction. DNA replica ...
Gene Set Enrichment Analysis
Gene Set Enrichment Analysis

... point where most different from zero Most significantly down-regulated genes ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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