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Why Should Our Community Care?
Why Should Our Community Care?

... The Bug in the Code ...
Genetic Engineering Techniques
Genetic Engineering Techniques

... additional  content.  New  enzymes  are  added  to  help  cement  the  new  linkages,  and   the  culture  is  then  separated  by  molecular  weight.  Those  molecules  that  weigh  the   most  have  successfully  incorporated  the  new ...
Human Genome Project, Stem Cells and Cloning
Human Genome Project, Stem Cells and Cloning

... that are accessible to the public 4. Analyzing and addressing ethical, legal, & social issues involved in ...
IGEM BOOT CAMP
IGEM BOOT CAMP

... mostly of one large circle of DNA 4-5 million base pairs (mbp) in length, with small loops of DNA called plasmids, usually ranging from 5,000-10,000 base pairs in length, present in the cytoplasm. It is these plasmids that bacteria can transfer back and forth, allowing them to share genes among one ...
Tmm - OpenWetWare
Tmm - OpenWetWare

... Chip_Platform GPL96: Affymetrix GeneChip Human Genome U133 Array Set HG-U133A for 712X712 ...
Genetic Disorders
Genetic Disorders

... -symptoms include tall stature, and abnormal body proportions, may be infertile, have feminine features and less developed male features ...
Hardy Weinberg Equilibrium and Evolution
Hardy Weinberg Equilibrium and Evolution

... • Microevolution: evolution on the smallest scalea generation to generation change in the frequencies of alleles within a population • Hardy Weinberg Equilibrium: populations that do not undergo change to their gene pools are not presently evolving – frequency of alleles in that gene pool are consta ...
Finding a cancer-causing gene
Finding a cancer-causing gene

... with precision and of determining its structure. It is known that DNA, the genetic material, is a long molecular chain ``written” in an alphabet of 4 ``letters” (A, C, G and T, the initials of the four types of molecules which constitute the DNA chain). Genetic data banks index several billion of su ...
Nucleotide drug targets
Nucleotide drug targets

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Chapter 17 and 19: Review Questions

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Nucleotide drug targets.
Nucleotide drug targets.

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BACTERIAL GENETICS
BACTERIAL GENETICS

... from one type of bacteria and inject it into genetically different bacteria. When purified DNA is injected into the nucleus of a eukaryotic cell, the process is called transfection. Transfection is frequently used in genetic engineering procedures ...
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... Directions: Cut out the cards along the lines, mix the cards then put the square back together. ...
MOLECULAR BIOLOGY EXAM II
MOLECULAR BIOLOGY EXAM II

... this protein? Are the sequences from other organisms similar? Is it always made or only at certain times? How is the gene regulated? You have three people working for you, all are pretty handy in the lab. Outline a strategy for each to begin tackling one of these questions, (or another critical issu ...
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Mutations & Genetic Engineering

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You and your Genes.
You and your Genes.

... there parents in some way. • Brothers and sisters may be different to each other but they could also be very similar. • There are two chromosomes that determine a gender. A male has a X and Y chromosome and a female has two X chromosomes. ...
Genetic Mutations
Genetic Mutations

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... A retrovirus is a RNA virus. The virus injects its mRNA along with reverse transcriptase into host cells. The reverse transcriptase is use to make DNA from viral mRNA. Once a double stranded DNA is made, it embeds itself into the host genome and or uses host RNA polymerase to transcribe viral protei ...
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How DNA Controls the Workings of the Cell
How DNA Controls the Workings of the Cell

... diabetes has a defective DNA sequence that codes for the making of the insulin protein. Suppose a person has a mutation in their DNA and the first triplet for the insulin gene reads T A T but the normal gene reads T A G. a. What amino acid does the mutant DNA and the normal DNA code for and will the ...
GENETICS EXAM 3 FALL 2004 Student Name
GENETICS EXAM 3 FALL 2004 Student Name

... a) Means of selection (i.e., identifying bacteria that contain recombinant DNA molecules) b) Origin of replication c) lac z gene d) Cloning sites 14. Assume you have identified an RFLP that is closely linked to a gene in which you are interested. Which of the following approaches would this allow yo ...
A4.3.1HowDoChromosomesCarryInformation
A4.3.1HowDoChromosomesCarryInformation

... 6. Where are centromeres located on chromosomes? Make a sketch of a chromosome and indicate where its centromere is located. 7. Where are telomeres located on chromosomes? Make a sketch of a chromosome and indicate where its telomeres are located. 8. From the variation window, select one of the chro ...
042310_recombinant_DNA2
042310_recombinant_DNA2

... • Small size in comparison with host’s chromosomes (for easy manipulation) • Ability to replicate independently (so that a lot of copies could be generated) • A recognition sequence for a restriction enzyme (so that we can introduce our DNA of interest) • Reporter genes (to confirm we have successfu ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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