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PART III. PROTEIN SYNTHESIS SATISFIES: How DNA Makes It A
PART III. PROTEIN SYNTHESIS SATISFIES: How DNA Makes It A

... (white) DNA molecule used in the REPLICATION kit, and place it to the right of the "membrane", along with all the blue mRNA (messenger-RNA) nucleotides scattered next to it. This represents the contents of the nucleus. 4. Now, on the left side of the membrane (in the "cytoplasm"), place the "ribosom ...
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... • Repeats, pseudo-genes, introns, contamination of vectors, are very confusing. ...
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...  Mitochondria – another place within the cell, other than the nucleus, where DNA can be found  Chloroplasts - another place within the cell, other than the nucleus, where DNA can be found  Ribosomes – where proteins are made  Introns – another name for “junk DNA”  Exons – sections of DNA that c ...
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... essential for growth (under lab conditions)? • Using transposon mutagenesis, ~150 of the 517 genes could be knocked out; ~ 300 genes deemed essential (under lab conditions), which included: – ~100 of unknown function – Genes for glycolysis & ATP synthesis – ABC transporters – Genes for DNA replicati ...
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CRACKING THE CODE OF LIFE QUESTIONS

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... The water buttercup has leaves that are shaped differently depending on where the leaves develop. Leaves that grow submerged in water are threadlike and those that grow above the water are ...
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... of one or more nucleotides that is not indivisible by three, therefore resulting in a completely different amino acid sequence than what would be normal. The earlier in the sequence nucleotides are added or removed, the more altered the protein will be. 15. A sequence of nucleotides composing a segm ...
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... effect on the protein due to • The degeneracy of the code. Each amino acid may be coded for by more than one codon. A substitution mutation may change one codon but it may still code for the same amino acid; hence no effect (silent mutation). • A change in the codon may result in a different amino a ...
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... • Learn how to insert a gene into bacteria (Heat Shock) • Analyze how a gene can transform an organism and express that gene • Provide evidence that bacteria can take in foreign DNA in the form of a plasmid • Reinforce the following process: DNA  RNA  Protein  Trait • Observe how genes are regula ...
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Genetics - Mr. Coleman's Biology

... Protein synthesis is the process of taking the coded messages found on DNA and turning them into proteins. DNA is found inside the nucleus (in eukaryotes) and ribosomes (which make proteins) are found outside of the nucleus. In order to complete the process RNA is used as a messenger between the DNA ...
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... Protein synthesis is the process of taking the coded messages found on DNA and turning them into proteins. DNA is found inside the nucleus (in eukaryotes) and ribosomes (which make proteins) are found outside of the nucleus. In order to complete the process RNA is used as a messenger between the DNA ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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