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Zinc finger nucleases
Zinc finger nucleases

... Meganucleases • Meganucleases, found commonly in microbial species, have the unique property of having very long recognition sequences (>14bp) thus making them naturally very specific. ...
doc BIOL202-16
doc BIOL202-16

... pUC18 vector also have a laz gene (different) than pUC18, a pUC18 vector that has not underwent successful recombination will have the lac Z gene intact because the gene of interest was not inserted into the lac Z gene. In the presence of the dye X-gal, transformed bacteria with a pUC18 vector that ...
Chapter Summary 3 - Genetics
Chapter Summary 3 - Genetics

All life is based on the same genetic code
All life is based on the same genetic code

... DNA is coiled tightly into an x-like called a chromosome stored in the nucleus of every cell. ...
Multiple choice questions
Multiple choice questions

... are not very common in the human genome can be used in genetic mapping of genomes are usually longer than 200 bp are normally found at the end of chromosomes Transcriptomes consist of RNA consist of DNA consist of proteins are translated into proteins do not change differ in different cells ...
Advanced Biology Vocabulary
Advanced Biology Vocabulary

... Autopolyploid An individual that has more than two chromosome sets that are all derived from a single species. ...
Genomics – The Language of DNA
Genomics – The Language of DNA

... VNTR may result from unequal crossover. It is the molecular basis of DNA fingerprinting which has many practical applications ...
Cloning Power Point
Cloning Power Point

... multiple copies of that gene using bacterial plasmids, selfreplicating extra-chromosomal circular DNA molecules, that are distinctly different from the normal bacterial genome. Genes and other chromosomes are copied to make enough samples for further study. In order to clone a gene, a fragment of DN ...
power point presentation
power point presentation

Chapter 11: Organization of DNA in Eukaryotes 11.2: mtDNA
Chapter 11: Organization of DNA in Eukaryotes 11.2: mtDNA

... Describe the Endosymbiotic hypothesis. Essentially, modern cells are a product of ancient eukaryotes engulfing free-living mitochondria and/or chloroplasts, allowing these (believed to be) prokaryotes to reside inside of the cytoplasm in a symbiotic relationship. After some time, these mitochondria ...
Crossing natural barriers to genetic manipulations
Crossing natural barriers to genetic manipulations

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CHNOPS Lab

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Day 2 (Jan. 23) Scribe Notes

... a “true nucleus”. Prokaryotes (such as bacteria) do not have nuclei or ribosomes. Moreover, their DNA includes no introns. This may help them evolve faster by causing more variation among their genotypes. Eukaryotic genomes, by contrast, are more robust and stable. A particular region of DNA does no ...
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PHYSgeneticsnotes

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AP Bio Review - Genetics Jeopardy

... express the trait. Jane is an achondroplastic dwarf. Matthew and Jane are planning a family of several children and want to know the chances of producing a child with achondroplastic dwarfism. If three children are born to Matthew and Jane, what are the chances that the first two children will not e ...
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NUTRIGENOMICA
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... for some of these differences and also promises us the ability to tailor diet based on individual genetic makeup. Great advances in Human Genome Project, documentation of single nucleotide polymorphisms (SNPs) in candidate genes and their association with metabolic imbalances have gradually added ne ...
nutrigenomica
nutrigenomica

... for some of these differences and also promises us the ability to tailor diet based on individual genetic makeup. Great advances in Human Genome Project, documentation of single nucleotide polymorphisms (SNPs) in candidate genes and their association with metabolic imbalances have gradually added ne ...
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LabM3bioinformatics

... As the proteins with similar functions contain homologus amino acid sequences that corresponds to important functional domains in the three dimensional structure of the proteins, so the function of a protein that is not been isolated often can be predicted based on the homology of its gene or cDNA w ...
Gene mutations and their effects
Gene mutations and their effects

... bases occur next to each other along one of the two DNA strands, they can become linked to form a thymine dimer. The dimer can be replicated as a single base, which results in a frameshift, possibly mutation, possibly resulting in skin cancer. • Chemicals – there are hundreds of chemical mutagens ...
Pierce chapter 10
Pierce chapter 10

... • Slightly acidic, high phosphorous content • Consisted of DNA and protein – Called in “nuclein” – later renamed nucleic acid ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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