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Slides
Slides

... make you – But only certain genes are transcribed and translated in specific cells – We call this EXPRESSION. • Muscle cells make the proteins that make them muscle cells (eg, the contractile filaments) • Kidney cells make the proteins that make them kidney cells (their shape and function is complet ...
GENETICS – BIO 300
GENETICS – BIO 300

... element family many other families discovered in maize autonomous elements encode information necessary for the transposition of themselves and nonautonomous members of their family ...
Prenatal Testing for Genetic Disorders
Prenatal Testing for Genetic Disorders

... 14.3 Transgenic Animals as Models of Human Diseases Mouse models of human diseases  Transfer of disease-causing human genes into mice creates transgenic organisms that are used to produce an animal with symptoms that mirror those in human study the development & progress of the diseases and tes ...
when a woman is color blind ______.
when a woman is color blind ______.

... all the genes for the male phenotype are found on the Y chromosome the amount of estrogen produced by XY cells is too small to activate the genes directing female development, causing the embryo to become male the Y chromosome carries a gene for testes formation; the testes then produce testosterone ...
Regulation of Gene Transcription
Regulation of Gene Transcription

... Basics and Logic of prokaryotic transcription regulation: A great example of regulation of gene transcription comes from prokaryotes and their regulation of metabolic enzymes to break down sugars (lactose, glucose, etc.) if all enzymes where transcribed it would be too energetically costly Therefore ...
Protocol S1
Protocol S1

... putative GI width must be over 15 kb; 2) the G+C% of the potential GI must have over 4.5 differences greater or smaller than the G+C% of S. suis P1/7. To determine possible sources of horizontally transferred GIs, we performed BLASTN. If the hit nucleotide sequence had >80% identity with the possibl ...
Manipulating DNA extracting and studying DNA
Manipulating DNA extracting and studying DNA

... used to make many copies of a desired gene.  Steps to PCR 1. DNA strands are separated by heating. 2. Short pieces of DNA, called primers, are attached to the DNA strands to prepare a place for DNA polymerase to start copying. 3. These copies serve as templates for more copies. ...
plasmids - genemol de Jean
plasmids - genemol de Jean

... Integrons are mobile DNA elements able to capture genes, notably those encoding antibiotic resistance, by site-specific recombination. Class 1 integrons have been examined the most extensively. They consist of a variable region bordered by 5' and 3' conserved regions. The 5' region is made up of th ...
PCR - churchillcollegebiblio
PCR - churchillcollegebiblio

2.4 review
2.4 review

... 29. Amino acids are linked together by condensation to form polypeptides. 30. There are 20 different amino acids in polypeptides synthesized on ribosomes. 31. The sequence of amino acids in polypeptides is coded for by genes. 32. A protein may consist of one or more than one polypeptide linked toget ...
Sex bias in gene expression is not the same as dosage
Sex bias in gene expression is not the same as dosage

... males (ZZ) than in females (ZW). It remains to be understood how they can cope with the overall imbalance in sex-linked gene expression. However, just as selection at individual loci across the genome has led to differences in expression levels between the sexes, there might of course also be loci o ...
As Powerpoint Slide
As Powerpoint Slide

DNA
DNA

... Griffith concluded that there was some factor that was passed from the S strain to the R strain that enabled the R strain to cause pneumonia Griffith called this process transformation. Also, since the ability to cause pneumonia was transferred to bacterial offspring, the factor for transformation m ...
The Central Dogma: A Journey Down a One
The Central Dogma: A Journey Down a One

... Both cells have to do some things the same, like respiration and protein synthesis and dividing. But other functions will be different, for example the heart cell has to contract and probably uses glucose faster for the energy needed for contraction. And skin cells need to do other things like makin ...
chapter 3 outline
chapter 3 outline

... RNA differs from DNA. -single-stranded -uracil instead of thymine -ribose instead of deoxyribose For any gene there is a transcribed strand (template) and a non-template strand. RNA polymerase makes RNA in a 5’ to 3’ direction, directed by a template, which is anti-parallel to the transcript. Initia ...
Distinguishing cell types with masks
Distinguishing cell types with masks

... a technique called fluorescent non-canonical amino acid tagging (FUNCAT) that allows visualization of changes in protein synthesis in the proteome on the time scale of minutes. “We know that some synaptic proteins can be synthesized only in the soma whereas some synaptic proteins appear to have a lo ...
Gene Mutations
Gene Mutations

... O severe cognitive, speech, and motor delays. O behavioral problems such as hyperactivity, aggression, tantrums, and repetitive movements. O unusual facial features which may change over time. O excessive drooling. ...
Bacterial and Viral Genetics
Bacterial and Viral Genetics

... “conjugation bridge”- a tube extends from the F+ (donor) bacterium to the F(recipient) bacterium  The donor’s plasmid separates, and a complimentary piece travels across the bridge to the recipient bacterium  A complimentary strand is produced by the ...
DNA and RNA are nucleic acids that carry out cellular
DNA and RNA are nucleic acids that carry out cellular

... a membranous envelope, but rather free-floating within the cytoplasm. The entire genetic content of a cell is known as its genome and the study of genomes is genomics. In eukaryotic cells, but not in prokaryotes, DNA forms a complex with histone proteins to form chromatin, the substance of eukaryoti ...
DNA and RNA are nucleic acids that carry out cellular
DNA and RNA are nucleic acids that carry out cellular

... a membranous envelope, but rather free-floating within the cytoplasm. The entire genetic content of a cell is known as its genome and the study of genomes is genomics. In eukaryotic cells, but not in prokaryotes, DNA forms a complex with histone proteins to form chromatin, the substance of eukaryoti ...
European green crabs (Carcinus maenas) are considered one of the
European green crabs (Carcinus maenas) are considered one of the

... “European green crabs (Carcinus maenas) are considered one of the world's worst invasive species...” European green crabs (Carcinus maenas) are considered one of the world's worst invasive species, and have been highly successful across a wide range of temperatures – from Newfoundland to Australia. ...
Chapter 4 - Cellular Metabolism
Chapter 4 - Cellular Metabolism

... Each new DNA molecule consists of one parental strand and one newly synthesized strand of DNA. ...
ESSAY 1: CONCEPTION
ESSAY 1: CONCEPTION

Bacterial Genetics
Bacterial Genetics

... Techniques used to isolate rare mutants in a population consisting mainly of wild-type cells Can select for antibiotic resistant mutants or auxotrophs ...
Biosynthesis of Bromocoumaric Acid in Bromoalterochromide A.
Biosynthesis of Bromocoumaric Acid in Bromoalterochromide A.

... incubated overnight and then isolated gDNA. We then grew E. Coli containing pHis8 cultured in 5mL of LB broth with the antibiotic Kanamycin (Kan). After isolating the plasmid we ran a restriction digestion on the pHis8 and isolated the linear plasmid. We then took the gDNA from P. piscicida and ran ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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