Water SA
... SYBR Green is a non-specific dye which binds to any double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based ...
... SYBR Green is a non-specific dye which binds to any double-stranded DNA. Therefore, it will not only bind to PCR products but to non-specific products that have been amplified, as well as primer dimers. Due to this non-specificity, SYBR Green requires more time for optimisation than protocols based ...
Homology Modelling
... Aim is to find the fold of native protein by simulating the biological process of protein folding. A VERY DIFFICULT task because a protein chain can fold into millions of different conformations. Use it only when no detectable homologues are available. ...
... Aim is to find the fold of native protein by simulating the biological process of protein folding. A VERY DIFFICULT task because a protein chain can fold into millions of different conformations. Use it only when no detectable homologues are available. ...
A-level Human Biology Question paper Unit 5 - Inheritance
... (b) In mice, two genes affecting coat colour are on different chromosomes. One gene controls whether there is any black pigment in the hairs. The dominant allele of this gene, B, results in black fur. The recessive allele, b, results in white fur. The second gene controls banding of the fur. The dom ...
... (b) In mice, two genes affecting coat colour are on different chromosomes. One gene controls whether there is any black pigment in the hairs. The dominant allele of this gene, B, results in black fur. The recessive allele, b, results in white fur. The second gene controls banding of the fur. The dom ...
Dr Noor Lita Adam 260609
... of thyroid hormonogenesis tend to have an autosomal recessive form of inheritance consistent with a single gene mutation. It is not surprising, therefore, that a molecular basis for many of these abnormalities has now been identified. These include mutations in the genes for the TSH receptor, sodium ...
... of thyroid hormonogenesis tend to have an autosomal recessive form of inheritance consistent with a single gene mutation. It is not surprising, therefore, that a molecular basis for many of these abnormalities has now been identified. These include mutations in the genes for the TSH receptor, sodium ...
Application Note 129 – Eppendorf MixMate
... yesterday they were still using 0.5 ml PCR tubes, now they are using 96- or 384-well PCR plates. Whereas only a few years ago, 10 ml tubes were still being used for bacterial cultures, now deepwell plates are the norm. However, these new formats and their special geometries, together with the smalle ...
... yesterday they were still using 0.5 ml PCR tubes, now they are using 96- or 384-well PCR plates. Whereas only a few years ago, 10 ml tubes were still being used for bacterial cultures, now deepwell plates are the norm. However, these new formats and their special geometries, together with the smalle ...
The Structure and Function of Large Biological Molecules
... 2. Carbohydrates serve as fuel and building material 3. Lipids are a diverse group of hydrophobic molecules 4. Proteins include a diversity of structures, resulting in a wide range of functions 5. Nucleic acids store, transmit, and help express hereditary information ...
... 2. Carbohydrates serve as fuel and building material 3. Lipids are a diverse group of hydrophobic molecules 4. Proteins include a diversity of structures, resulting in a wide range of functions 5. Nucleic acids store, transmit, and help express hereditary information ...
Eurofins Legionella PCR (Polymerase Chain Reaction) Technical
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
... Advantages of Using the Legionella PCR Method Rather than the Culture Technique The traditional Legionella culture technique takes 10 days to arrive at a confirmed negative result as Legionella bacteria are slow growing in the laboratory. A further 3 days are required for confirmation in the case of ...
Invagination centers within the Drosophila stomatogastric nervous
... Fig. 1. The SNS of late Drosophila embryos. (A) Diagram illustrating the components of the SNS (red) and their relative positions with respect to morphological landmarks such as different portions of the gut and brain as seen by mAb22C10 or anti-fasciclin II antibody stainings (B-H); anterior is lef ...
... Fig. 1. The SNS of late Drosophila embryos. (A) Diagram illustrating the components of the SNS (red) and their relative positions with respect to morphological landmarks such as different portions of the gut and brain as seen by mAb22C10 or anti-fasciclin II antibody stainings (B-H); anterior is lef ...
Math of Genetics - College of William & Mary
... Pepper color is controlled by two different genes The first gene controls the expression of red pigment The dominant allele (R) indicates the presence of red ...
... Pepper color is controlled by two different genes The first gene controls the expression of red pigment The dominant allele (R) indicates the presence of red ...
- Wiley Online Library
... which can be produced in an industrial scale. However, PHB is a rather brittle and inflexible biopolymer which compromised in its range of applications. To increase the utility of these biopolymers it is necessary to produce PHAs with different monomer compositions, e.g. longer chain length for impr ...
... which can be produced in an industrial scale. However, PHB is a rather brittle and inflexible biopolymer which compromised in its range of applications. To increase the utility of these biopolymers it is necessary to produce PHAs with different monomer compositions, e.g. longer chain length for impr ...
meiosis
... – If pea plant, for example, produces 14 chromosomes – Gametes would each contain 14 chromosomes – Each offspring would have 28 – Offspring of next generation would have 56 ...
... – If pea plant, for example, produces 14 chromosomes – Gametes would each contain 14 chromosomes – Each offspring would have 28 – Offspring of next generation would have 56 ...
Aucsia Gene Silencing Causes Parthenocarpic Fruit Development in
... DefH9-iaaM and DefH9-RI-iaaM flower buds are morphologically identical to wild-type buds, except for the ovaries, which are more enlarged than those of controls. To identify differentially expressed genes, we performed a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis using 32 diffe ...
... DefH9-iaaM and DefH9-RI-iaaM flower buds are morphologically identical to wild-type buds, except for the ovaries, which are more enlarged than those of controls. To identify differentially expressed genes, we performed a cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis using 32 diffe ...
BMC Microbiology
... Results: The strains produced fatty acid patterns typical for members of the family Enterobacteriaceae. Comparative sequence analyses of the 16S rRNA as well as rpoB genes allocated the strains to two well-defined groups within the genus Enterobacter, family Enterobacteriaceae. The analyses indicate ...
... Results: The strains produced fatty acid patterns typical for members of the family Enterobacteriaceae. Comparative sequence analyses of the 16S rRNA as well as rpoB genes allocated the strains to two well-defined groups within the genus Enterobacter, family Enterobacteriaceae. The analyses indicate ...
Photoreceptor Biotechnology Matthew Hudson Department of Crop
... The ability to alter at will the time of year at which a crop flowers or is ready for harvest has obvious potential for crop improvement. By the same means, depending on the organism, it may be possible to control the timing of fruit ripening, tuberization, grain filling and other related traits. Ov ...
... The ability to alter at will the time of year at which a crop flowers or is ready for harvest has obvious potential for crop improvement. By the same means, depending on the organism, it may be possible to control the timing of fruit ripening, tuberization, grain filling and other related traits. Ov ...
Mechanisms of Transcription-Replication
... the elongation rate for RNA polymerase is just 50 nucleotides (nt) per s (15); i.e., replication is approximately 20-fold faster than transcription. Since the two processes proceed simultaneously, frequent collisions between replication and transcription seem unavoidable (3, 38). Given that both pro ...
... the elongation rate for RNA polymerase is just 50 nucleotides (nt) per s (15); i.e., replication is approximately 20-fold faster than transcription. Since the two processes proceed simultaneously, frequent collisions between replication and transcription seem unavoidable (3, 38). Given that both pro ...
Molecular cloning, characterization and gene expression of an
... 2.4. Gene expression analysis of MrCat mRNA after IHHNV infection For IHHNV induced mRNA expression analysis, the prawns were injected with IHHNV, as described by Dhar et al. [51]. Briefly, IHHNV infected prawn tail tissue, tested positive by nested PCR was homogenized in sterile 2% NaCl (1:10, w/v) ...
... 2.4. Gene expression analysis of MrCat mRNA after IHHNV infection For IHHNV induced mRNA expression analysis, the prawns were injected with IHHNV, as described by Dhar et al. [51]. Briefly, IHHNV infected prawn tail tissue, tested positive by nested PCR was homogenized in sterile 2% NaCl (1:10, w/v) ...
Color and Scent: How Single Genes Influence Pollinator Attraction
... This raises the question of the genetic basis of the evolution of pollination syndromes. If each of the individual traits is encoded by multiple genes that must coordinately evolve, how is it possible that switches among syndromes happen frequently? To answer this question, it is necessary to identi ...
... This raises the question of the genetic basis of the evolution of pollination syndromes. If each of the individual traits is encoded by multiple genes that must coordinately evolve, how is it possible that switches among syndromes happen frequently? To answer this question, it is necessary to identi ...
Gene Name
... encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes. Same as APEX1 or APEX nuclease (multifunctional DNA repair enzyme) 1. Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that ...
... encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes. Same as APEX1 or APEX nuclease (multifunctional DNA repair enzyme) 1. Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that ...
The coiled-coil of the human Rad50 DNA repair protein contains
... globular ATPase domain made up from the N and C termini of the protein separated by an extensive central region predicted to form a coiled-coil (2). Accumulated recent evidence reveals that Rad50 and other SMC proteins are arranged as intramolecular coiled-coils bringing together the N and C termini ...
... globular ATPase domain made up from the N and C termini of the protein separated by an extensive central region predicted to form a coiled-coil (2). Accumulated recent evidence reveals that Rad50 and other SMC proteins are arranged as intramolecular coiled-coils bringing together the N and C termini ...