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Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry

... also indicated an important role of soluble glutamine as a nitrogen-transporting compound (12). In this report, only protein glutamine was determined. Glutamine accumulation correlated with the accumulation of cytosolic glutamine synthetase (11). Other researchers suggest that several amino acids, a ...
Number 53, 2006 5 m sh4
Number 53, 2006 5 m sh4

... contain MutS DNA-binding and ATPase domains. MSH4 also seems to have a role in the formation or elongation of the synaptonemal complex, a proteinaceous structure that holds homologous chromosomes in close proximity prior to the reductional division of meiosis. Chromosome synapsis is delayed and inco ...
Protein Foods
Protein Foods

... o Complete: Animal sources of protein. These proteins contain all of the protein building blocks called amino acids that your body needs to grow and maintain tissue. o Incomplete: Plant sources of protein. These proteins are missing one or more amino acids; not all of the building blocks are there. ...
Molecular Cloning and Expression of a 2-Arylpropionyl
Molecular Cloning and Expression of a 2-Arylpropionyl

... was detected in the liver and kidney. A very low expression was observed in the heart and brain. ...
The role of mutations in core protein of hepatitis B
The role of mutations in core protein of hepatitis B

... corresponded to the part of core protein that interact closely with RNA [5]. In this domain phosphorylated site residues located in amino acid sequences 155-183. Immature nucleocapsids which contain RNA are phosphorylated at six sites, while the mature nucleocapsids which contain DNA are completely ...
Programming for Bioinformatics
Programming for Bioinformatics

... Two approaches: Blast vs. DIY  We can answer the above easily:  We use SCOP database and run database queries from a Python script For a given superfamily select all corresponding sequences from the astral table For all pairs of selected sequences  Call Blast and record the sequence identity  ...
Editing Multiple Alignments
Editing Multiple Alignments

... Generating multiple alignments  Pairwise sequence alignment is easy with sufficiently closely related sequences.  Below a certain level of identity sequence alignment may become uncertain : twilight zone for aa sequences ~ 30%. ...
Part 2
Part 2

... 2. Immunoblotting: This process, also known as Western blotting, is a commonly used analytical technique for detection of specific proteins in a given mixture by means of specific antibodies to the given target protein. a) Electrophoresis: Electrophoresis is a gel-based analytical technique that is ...
Sequence Alignment
Sequence Alignment

... • exactly the same Amino Acid or Nucleotide in the same position Sequence similarity • substitutions with similar chemical properties Sequence homology • general term that indicates evolutionary relatedness among sequences • sequences are homologous if they are derived from a common ancestral sequen ...
The Plant Cell
The Plant Cell

... as the BLAST query. Sequencing revealed that each EST contained a substantial untranslated region 5  to the coding region without any long open reading frame. The 3  untranslated region of each EST contained a poly(A) tail. The corresponding genomic sequence in the GenBank database also suggested ...
minervini
minervini

... Enabling Grids for E-sciencE ...
Can sequence determine function? | Genome Biology | Full Text
Can sequence determine function? | Genome Biology | Full Text

... by crotonase has been subjected to mechanistic scrutiny [2022]; several high-resolution structures are available for the rat mitochondrial enzyme [23,24]. As a result of these studies, the mechanism is known to involve two glutamate residues that function as acid/base catalysts: in the direction of ...
AMINO ACIDS I. Function of amino acids A. Building blocks of
AMINO ACIDS I. Function of amino acids A. Building blocks of

... a. Hydroxyproline and hydroxylysine i. hydroxylated enzymatically after translation ii. important in collagen structure b. phosphoamino acids i. Tyr, Ser and Thr hydroxyl groups can be phosphorylated ii. important in activation and inhibition of enzymatic activity c. Cysteine can form disulfide bond ...
Nucleotide Sequence of the Gene Coding for the
Nucleotide Sequence of the Gene Coding for the

... not conserved, and are these regions responsible for the obN2 3H2+ 2NH3; the detailsof its mechanism are notknown served differences in the nitrogenase proteins? If this is true, (1). The enzyme system has been isolated from at least 10 may it be possible, by DNA recombination in vitro, to condiffer ...
Purification and proteomic characterization of plastids from Brassica
Purification and proteomic characterization of plastids from Brassica

... ice to separate nonmacerated plant material from protoplasts. The 150 mm nylon mesh flow through was then passed three consecutive times through one layer of a 60-mm nylon mesh into a fresh tube on ice. This step was performed to gently lyse the protoplasts and was assisted by gently forcing the pro ...
Protein Folding Cell and Mol Biology Lab
Protein Folding Cell and Mol Biology Lab

... (see end of this file for selected text pages on CDK2/cyclin, Calmodulin, protein folding diseases, prions and chaperones Protein Structure Sites: Protein Folding (Chapt. 3 of World of the Cell) and Enzymes (Chapt. 6): How a protein folds in 3D space is important for protein function. If a protein d ...
Protein-only inheritance in yeast: something to get
Protein-only inheritance in yeast: something to get

... Sup35p–GFP is briefly expressed in [psi2] strains, its fluorescence is distributed diffusely throughout the cell. However, if Sup35p–GFP is expressed in [PSI1] strains, the fluorescence coalesces into foci as soon as it can be visualized, suggesting that pre-existing complexes of Sup35p in [PSI1] ce ...
Identification of several different lineages of measles virus
Identification of several different lineages of measles virus

... Viruses. The origin and nucleotide sequence of some of the virus strains analysed here have been reported earlier (See Table 1). The origins of strains EdP9, Hu2, MVO and MVP have been described before (Rima et al., 1983). Sequencesof the $33 and $81 strains were obtained from brain autopsy material ...
Discovery, Structural Determination, and Putative
Discovery, Structural Determination, and Putative

... circular proteins potentially have a range of advantages over conventional proteins and may find interesting applications in protein engineering, agriculture, and drug design (4). SFTI-12 is a 14-amino-acid, backbone-cyclized protein found in the seeds of Helianthus annus (sunflower), which possesse ...
Protein thermostability in Archaea and Eubacteria
Protein thermostability in Archaea and Eubacteria

... mophiles shows that the number of charged amino acids (which may include Lys) is very high in hyperthermophiles, whereas thermophiles have a preference of Arg over Lys. However, Asp and Met are unstable at high temperatures and therefore their percentage decreases in hyperthermophiles (Szilágyi and ...
Har Gobind Khorana - Pontifical Academy of Sciences
Har Gobind Khorana - Pontifical Academy of Sciences

... broad interests in biological problems and they received wide recognition by the scientific community. By the 1950s his laboratory had already been studying energy-rich phosphate esters including polynucleotides. He thereby developed methods for their synthesis. He continued this work after moving t ...
GST II: ---Title--- - Digital Biology Laboratory
GST II: ---Title--- - Digital Biology Laboratory

... problem for strings, (Robinson, 1938) LCS: define a set of operations (e.g. substitution, insertion or deletion) that transform the aligned elements of one sequence into the corresponding elements of the other and associate with each operation a cost or a score. Optimal alignment: the alignment that ...
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen
1 A Comparative, Double-blind, Triple Crossover Net Nitrogen

... total consumption of each designated portion was reached. ...
Role of Water Mediated Interactions in Protein
Role of Water Mediated Interactions in Protein

... The importance of a heterogeneous environment for proteinligand thermodynamics has long been recognized.2,6 Effective interactions between for example two residues will by necessity be modulated by the presence of, e.g., other residues in a given molecule as well as solvent molecules. A biological c ...
Sure, some prions can cause diseases, but others are
Sure, some prions can cause diseases, but others are

... a drastically different environment. Various prions in the waterlogged yeast cells will switch from inactive to active, or active to inactive, to ensure that some of the cells survive under­ water. Some of those activation switches help the yeast, but some do not. Jonathan Weissman, an HHMI investig ...
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Ancestral sequence reconstruction

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